Development of chitosan nanoparticle loaded with Tricholoma fracticum extract and evaluation of in vitro antioxidant activity

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dc.authoridCANBOLAT, FADIME/0000-0001-6759-7735
dc.contributor.authorCanbolat, Fadime
dc.contributor.authorAcar, Ismail
dc.contributor.authorTezel, Ruhiye Nilay
dc.date.accessioned2025-01-27T20:27:09Z
dc.date.available2025-01-27T20:27:09Z
dc.date.issued2024
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThe objective of this study was to develop Tricholoma fracticum extract-loaded chitosan nanoparticles (TFNPs) by ionic gelation method and to evaluate their in vitro antioxidant activity. Phenolic and flavonoid contents in the T. fracticum extract were measured spectrophotometrically and chromatographically. Characterisation of NPs was evaluated by field emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM), ZETA analysis, Fourier-transform infrared spectroscopy (FT-IR), UV-visible spectroscopy (UV-Vis) and thermogravimetric analysis (TGA). In vitro antioxidant capacity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2 '-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. The phenolic and flavonoid contents in the T. fracticum extract were measured as 7.1 +/- 0.3 mg Gallic Acid Equivalent/g extract and 5.5 +/- 0.6 mg Quercetin Equivalent/g extract, respectively. The particle size, polydispersity index (PDI) and zeta potential of Blank NP1 and TFNP were 265.5 +/- 15.8 nm, 0.4, 38.7 +/- 4.0 mV and 333.2 +/- 16.3 nm, 0.4, 37.0 +/- 4.1 mV, respectively. The highest antioxidant activity was observed in TFNP, followed by T. fracticum extract, chitosan and blank NP, respectively. The preserved or enhanced antioxidant activity observed in the encapsulated T. fracticum extract indicates the potential for loading similar mushroom extracts onto chitosan and thus preserving their bioactive properties.
dc.identifier.doi10.1111/ijfs.17585
dc.identifier.endpage7986
dc.identifier.issn0950-5423
dc.identifier.issn1365-2621
dc.identifier.issue10
dc.identifier.scopus2-s2.0-85203422729
dc.identifier.scopusqualityQ1
dc.identifier.startpage7971
dc.identifier.urihttps://doi.org/10.1111/ijfs.17585
dc.identifier.urihttps://hdl.handle.net/20.500.12428/22602
dc.identifier.volume59
dc.identifier.wosWOS:001309361000001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofInternational Journal of Food Science and Technology
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectAntioxidant
dc.subjectcharacterisation
dc.subjectchitosan nanoparticle
dc.subjectchromatography
dc.subjectionic gelation
dc.subjectTricholoma fracticum
dc.titleDevelopment of chitosan nanoparticle loaded with Tricholoma fracticum extract and evaluation of in vitro antioxidant activity
dc.typeArticle

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