Development of chitosan nanoparticle loaded with Tricholoma fracticum extract and evaluation of in vitro antioxidant activity
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Tarih
2024
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The objective of this study was to develop Tricholoma fracticum extract-loaded chitosan nanoparticles (TFNPs) by ionic gelation method and to evaluate their in vitro antioxidant activity. Phenolic and flavonoid contents in the T. fracticum extract were measured spectrophotometrically and chromatographically. Characterisation of NPs was evaluated by field emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM), ZETA analysis, Fourier-transform infrared spectroscopy (FT-IR), UV-visible spectroscopy (UV-Vis) and thermogravimetric analysis (TGA). In vitro antioxidant capacity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2 '-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. The phenolic and flavonoid contents in the T. fracticum extract were measured as 7.1 +/- 0.3 mg Gallic Acid Equivalent/g extract and 5.5 +/- 0.6 mg Quercetin Equivalent/g extract, respectively. The particle size, polydispersity index (PDI) and zeta potential of Blank NP1 and TFNP were 265.5 +/- 15.8 nm, 0.4, 38.7 +/- 4.0 mV and 333.2 +/- 16.3 nm, 0.4, 37.0 +/- 4.1 mV, respectively. The highest antioxidant activity was observed in TFNP, followed by T. fracticum extract, chitosan and blank NP, respectively. The preserved or enhanced antioxidant activity observed in the encapsulated T. fracticum extract indicates the potential for loading similar mushroom extracts onto chitosan and thus preserving their bioactive properties.
Açıklama
Anahtar Kelimeler
Antioxidant, characterisation, chitosan nanoparticle, chromatography, ionic gelation, Tricholoma fracticum
Kaynak
International Journal of Food Science and Technology
WoS Q Değeri
N/A
Scopus Q Değeri
Q1
Cilt
59
Sayı
10
