Chitosan Nanoparticles Loaded with Quercetin and Valproic Acid: A Novel Approach for Enhancing Antioxidant Activity against Oxidative Stress in the SH-SY5Y Human Neuroblastoma Cell Line

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dc.authoridDemir, Neslihan/0000-0002-2347-8344
dc.authoridTONGUC YAYINTAS, Ozlem/0000-0002-3554-1881
dc.authoridSenel, Mehmet/0000-0001-7366-4746
dc.authoridKilicaslan Ayna, Tulay/0000-0001-7993-978X
dc.authoridCANBOLAT, FADIME/0000-0001-6759-7735
dc.contributor.authorCanbolat, Fadime
dc.contributor.authorDemir, Neslihan
dc.contributor.authorYayintas, Ozlem Tonguc
dc.contributor.authorPehlivan, Melek
dc.contributor.authorEldem, Asli
dc.contributor.authorAyna, Tulay Kilicaslan
dc.contributor.authorSenel, Mehmet
dc.date.accessioned2025-01-27T20:51:53Z
dc.date.available2025-01-27T20:51:53Z
dc.date.issued2024
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractBackground: Multiple drug-delivery systems obtained by loading nanoparticles (NPs) with different drugs that have different physicochemical properties present a promising strategy to achieve synergistic effects between drugs or overcome undesired effects. This study aims to develop a new NP by loading quercetin (Que) and valproic acid (VPA) into chitosan. In this context, our study investigated the antioxidant activities of chitosan NPs loaded with single and dual drugs containing Que against oxidative stress. Method: The synthesis of chitosan NPs loaded with a single (Que or VPA) and dual drug (Que and VPA), the characterization of the NPs, the conducting of in vitro antioxidant activity studies, and the analysis of the cytotoxicity and antioxidant activity of the NPs in human neuroblastoma SH-SY5Y cell lines were performed. Result: The NP applications that protected cell viability to the greatest extent against H2O2-induced cell damage were, in order, 96 mu g/mL of Que-loaded chitosan NP (77.30%, 48 h), 2 mu g/mL of VPA-loaded chitosan NP (70.06%, 24 h), 96 mu g/mL of blank chitosan NP (68.31%, 48 h), and 2 mu g/mL of Que- and VPA-loaded chitosan NP (66.03%, 24 h). Conclusion: Our study establishes a successful paradigm for developing drug-loaded NPs with a uniform and homogeneous distribution of drugs into NPs. Chitosan NPs loaded with both single and dual drugs possessing antioxidant activity were successfully developed. The capability of chitosan NPs developed at the nanometer scale to sustain cell viability in SH-SY5Y cell lines implies the potential of intranasal administration of chitosan NPs for future studies, offering protective effects in central nervous system diseases.
dc.description.sponsorshipanakkale Onsekiz Mart University Scientific Research Projects Coordination Unit
dc.description.sponsorshipWe would like to thank Feyza Kolcu, Ruhiye Nilay Tezel, and Busra Dalg & imath;c for providing the laboratory facilities for the characterization analysis of NPs.
dc.identifier.doi10.3390/biomedicines12020287
dc.identifier.issn2227-9059
dc.identifier.issue2
dc.identifier.pmid38397889
dc.identifier.scopus2-s2.0-85186268955
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.3390/biomedicines12020287
dc.identifier.urihttps://hdl.handle.net/20.500.12428/25566
dc.identifier.volume12
dc.identifier.wosWOS:001172314100001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherMdpi
dc.relation.ispartofBiomedicines
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectantioxidant effect
dc.subjectchitosan
dc.subjectnanoparticle
dc.subjectoxidative stress
dc.subjectquercetin
dc.subjectvalproic acid
dc.titleChitosan Nanoparticles Loaded with Quercetin and Valproic Acid: A Novel Approach for Enhancing Antioxidant Activity against Oxidative Stress in the SH-SY5Y Human Neuroblastoma Cell Line
dc.typeArticle

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