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Öğe Trends of COVID 19 Vaccines: International Collaboration and Visualized Analysis(The Turkish Society Of Clinical Microbiology and Infectious Diseases, 2021) Alkan, Sevil; Öntürk, Hatice; Alıravcı, Işıl Deniz; Sıddıkoğlu, DuyguObjective: We aimed to evaluate the research and publication trends on the coronavirus disease (COVID-19) vaccine, and so guide future studies. Materials and Methods: A bibliometric analysis was performed using a VOSviewer visualization methodology. Information about publications Web of Science database outputs, countries, institutions, journals, keywords, and citation counts was retrieved. Results: A total of 929 eligible publications from January 1, 2020, to February 28, 2021, were derived from the WOS database according to the search criteria. Publications were written in nine languages, mainly in English (96.7%). From these results, a total of 300 articles were reached by filtering. The average number of citations was found to be 7.73. The H-index of the articles, which were cited 2320 times in total, was 22. Most of the publications were articles (32.29%) and editorials (28.09%). There were 73 different research areas, mostly in general internal medicine (26.6%) and immunology (19.05%). The United States of America produced the majority of articles (31.32%). Conclusion: This bibliometric analysis presents that publications on the COVID-19 vaccine are rapidly changing at a time when exactly the effective vaccines of COVID-19 have not been discovered yet.Öğe CT angiography prior to DIEP flap breast reconstruction: a randomized controlled trial(Elsevier Ltd., 2022) Çolakoğlu, Salih; Tebockhorst, Seth; Freedman, Jonathan; Douglass, Sara; Sıddıkoğlu, Duygu; Chong, Tae W.; Mathes, David W.Background: CT angiogram (CTA) has become the preferred method for the planning of abdominal-based microsurgical breast reconstruction to gather information about location, number, caliber and trajectory of the abdominal perforators and to decrease overall flap dissection and operating room time. However, the high-level evidence to support its utility has been limited to nonrandomized retrospective and prospective studies. Methods: Patients undergoing deep inferior epigastric artery perforator (DIEP) flap breast reconstruction were prospectively randomized to preoperative CTA and no imaging groups. Patient demographics, operative times, selected row and number of perforators for flap harvest, agreement in perforator selection between radiologist and surgeon, and clinical outcomes data were collected. Two-way ANOVA, Fisher's exact and Student's t-tests were used for statistical analysis. Results: Overall, 37 patients with 63 flaps were included in this study. Seventeen patients had CT scan prior to surgery. Mean age was 50.5 ± 9.6 years. Flap dissection time was significantly shorter in the CT group (150.8 ± 17.8 vs 184.7 ± 25.1 min and p< 0.001). Although overall odds ratio (OR) time was also shorter in the CT group, this only reached a statistical significance in bilateral surgeries (575.9 ± 70.1 vs 641.9 ± 79.6 min and p = 0.038). Hemiabdomen side, selected DIEP row, and the number of dissected perforators did not affect the overall dissection time. Complication rates were similar between the two groups. Conclusion: This prospective, randomized study demonstrates that preoperative CTA analysis of perforators decreases flap harvest and overall OR time with equivalent postoperative outcomes.Öğe Genetic variability in the prion protein gene in five indigenous Turkish sheep breeds(Elsevier, 2011) Alvarez, Lorena; Gutierrez-Gil, Beatriz; Uzun, Metehan; San Primitivo, Fermin; Jose Arranz, JuanOvine susceptibility to scrapie is largely controlled by polymorphisms in the PRNP gene. Beginning in 2003, breeding programmes based on the known association between this gene and the susceptibility/resistance status of this disease have been implemented in many European countries. This is not the case in Turkey, where the PRNP gene was not genotyped in native sheep breeds until recently. We sequenced the complete open reading frame of the PRNP gene in 100 sheep belonging to five native Turkish sheep breeds (Akkaraman = 21; Morkaraman = 20; Tuj = 17; Hemsin = 23; Karayaka = 19). Based on the variability found at codons 136, 154 and 171 (also referred to as standard codons), we determined six alleles VRQ TRQ ARR, ARH, ARK and ARQ. The archetype, ARQ was the most frequent variant in each of the five breeds (across-breed frequency = 0.710), while the second most frequent was the resistance-associated ARR allele (0.140). The susceptible VRQ allele exhibited the lowest frequency (0.015). The AHQ allele was not found in any of the analysed breeds. Beyond variability in the standard codons, we found ten additional amino acid variants (M112T, G127S, G127V, M137T, L141F, H143R, N146S, Y172D, Q189L and V213E), one of which, V213E, has not been previously described to our knowledge. Overall, this report may serve as a complement of previous studies on the genetic background of native Turkish sheep regarding the resistance/susceptibility status to classical and atypical scrapie. (C) 2011 Elsevier B.V. All rights reserved.Öğe P(4-VP) based nanoparticles and composites with dual action as antimicrobial materials(Elsevier Science Bv, 2010) Özay, Özgür; Alper Akçalı; Tatman Otkun, Müşerref; Sılan, Coşkun; Aktaş, Nahit; Şahiner, NurettinPolymeric 4-VP (p(4-VP)) particles were synthesized in an oil-in-water microemulsion system using various amounts of ethylene glycol dimethacrylate (EGDMA) as crosslinker. The prepared p(4-VP) particles were chemically modified to obtain positively charged particles as polyelectrolytes. Furthermore, these p(4-VP) particles were used for in situ Ag and Cu metal nanoparticle syntheses to provide dual action with an additional advantage as bactericidal particles. The synthesized p(4-VP) particles with positive charges and metal constituents were tested for potential antibacterial action against various bacteria such as Staphylococcus aureus ATCC6538, Pseduomonas aeruginosa ATCC9027, Bacillus subtilis ATCC6633, Escherichia coli ATCC8739. It was found that p(4-VP) particles, especially the positively charged forms had potential as antibacterial materials. The synthesized particle dimensions were characterized with TEM, and DLS measurements. Chemical modification of the particles was confirmed by FT-IR spectroscopy and zeta potential measurements, and the metal nanoparticle contents were determined with thermogravimetric (TGA) studies. (C) 2010 Elsevier B.V. All rights reserved.Öğe Milk hydrogels as nutrient media and survival rate enhancer under cryogenic conditions for different microorganisms(Springer, 2016) Alpaslan, Duygu; Şahiner, Mehtap; Yüceer, Yonca; Akçalı, Alper ; Aktaş, Nahit; Şahiner, NurettinMilk-containing hydrogels from 2-hydroxyethylmethacrylate (HEMA) and acrylamide (AAm) as p(HEMA)/whole milk, p(HEMA)/semi-skimmed milk, p(HEMA)/skimmed milk, p(AAm)/whole milk, p(AAm)/semi-skimmed milk, p(AAm)/skimmed milk were synthesized by conventional redox polymerization technique using corresponding monomers and milk as solvents. The aim of this study was to investigate the use of milk-containing hydrogels as a nutritional medium, e.g., up 124 days for enhanced or longer cryostorage media for various microorganisms such as Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus subtilis, and fungi, Aspergillus niger, Aspergillus fumigates, and a yeast Candida albicans. It was shown that milk hydrogels can be used as nutritional media for the growth of these microorganisms, whereas the control hydrogels cannot be used for the same purpose. The survival rates of p(HEMA)/whole milk and p(AAm)/whole milk hydrogels for E. coli after 124 days were found to exceed those of the control p(HEMA) and p(AAm) hydrogels by 109- and 56-fold, respectively. Moreover, the survival rates for S. aureus were calculated to be 42 and 24 times longer than the controls, as the hydrogels containing whole milk were used as storage media in comparison with the bare hydrogels. Furthermore, the cryogenic survival rates of p(HEMA)/whole milk and p(AAM)/whole milk were compared with whole milk and nutrient broth used as a control, and calculated to be 67-, 171-, 35-, and 89-fold greater, respectively, than those of the control samples for E. coli.Öğe Novel inhibitors of eukaryotic elongation factor 2 kinase: In silico, synthesis and in vitro studies(Academic Press Inc., 2021) Cömert Önder, Ferah; Durdagi, Serdar; Kahraman, Nermin; Uslu, Tuğce Nur; Kandemir, Hakan; Ay, MehmetEukaryotic elongation factor 2 kinase (eEF2K) is an unusual alpha kinase whose expression is highly upregulated in various cancers and contributes to tumor growth, metastasis, and progression. More importantly, eEF2K expression is associated with poor clinical outcome and shorter patient survival in breast, lung and ovarian cancers. Therefore, eEF2K is an emerging molecular target for development of novel targeted therapeutics and precision medicine in solid cancers. Currently, there are not any available potent and specific eEF2K inhibitors for clinical translation. In this study, we designed and synthesized a series of novel compounds with coumarin scaffold with various substitutions and investigated their effects in inhibiting eEF2K activity using in silico approaches and in vitro studies in breast cancer cells. We utilized an amide substitution at position 3 on the coumarin ring with their pharmacologically active groups containing pyrrolidine, piperidine, morpholine and piperazine groups with [sbnd](CH2)2[sbnd] bridged for aliphatic amides. Due to their ability to form covalent binding to the target enzyme, we also investigated the effects of boron containing groups on functionalized coumarin ring (3 compounds) and designed novel aliphatic and aromatic derivatives of coumarin scaffolds (10 compounds) and phenyl ring with boron groups (4 compounds). The Glide/SP module of the Maestro molecular modeling package was used to perform in silico analysis and molecular docking studies. According to our combined results, structure activity relationship (SAR) was performed in detail. Among the newly designed, synthesized, and tested compounds, our in vitro findings revealed that several compounds displayed a highly effective eEF2K inhibition at submicromolar concentrations in in vitro breast cancer cells. In conclusion, we identified novel compounds that can be used as eEF2K inhibitors and that they should be further evaluated by in vivo preclinical tumor models studies for antitumor efficacy and clinical translation.Öğe Prediction of mortality in COVID-19 through combing CT severity score with NEWS, qSOFA, or peripheral perfusion index(W.B. Saunders, 2021) Akdur, Gökhan; Daş, Murat; Bardakçı, Okan; Akman, Canan; Sıddıkoğlu, Duygu; Akdur, Okhan; Akçalı, Alper; Erbaş, Mesut; Reşorlu, Mustafa; Beyazıt, YavuzIntroduction: The assessment of disease severity and the prediction of clinical outcomes at early disease stages can contribute to decreased mortality in patients with Coronavirus disease 2019 (COVID-19). This study was conducted to develop and validate a multivariable risk prediction model for mortality with using a combination of computed tomography severity score (CT-SS), national early warning score (NEWS), and quick sequential (sepsis-related) organ failure assessment (qSOFA) in COVID-19 patients. Methods: We retrospectively collected medical data from 655 adult COVID-19 patients admitted to our hospital between July and November 2020. Data on demographics, clinical characteristics, and laboratory and radiological findings measured as part of standard care at admission were used to calculate NEWS, qSOFA score, CT-SS, peripheral perfusion index (PPI) and shock index (SI). Logistic regression and Cox proportional hazard models were used to predict mortality, which was our primary outcome. The predictive accuracy of distinct scoring systems was evaluated by the receiver-operating characteristic (ROC) curve analysis. Results: The median age was 50.0 years [333 males (50.8%), 322 females (49.2%)]. Higher NEWS and SI was associated with time-to-death within 90-days, whereas higher age, CT-SS and lower PPI were significantly associated with time-to-death within both 14 days and 90 days in the adjusted Cox regression model. The CT-SS predicted different mortality risk levels within each stratum of NEWS and qSOFA and improved the discrimination of mortality prediction models. Combining CT-SS with NEWS score yielded more accurate 14 days (DBA: −0.048, p = 0.002) and 90 days (DBA: −0.066, p < 0.001) mortality prediction. Conclusion: Combining severity tools such as CT-SS, NEWS and qSOFA improves the accuracy of predicting mortality in patients with COVID-19. Inclusion of these tools in decision strategies might provide early detection of high-risk groups, avoid delayed medical attention, and improve patient outcomes.Öğe Heavy Metal and Mineral Composition of Soil, Atmospheric Deposition, and Mosses with Regard to Integrated Pollution Assessment Approach(Springer, 2021) Menteşe, Sibel; Tonguç Yayıntaş, Özlem; Baş, Batuhan; İrkin, Latife Ceyda; Yılmaz, SelehattinThe fact that there are no real borders between the biosphere, atmosphere, lithosphere, and hydrosphere means that environmental pollution monitoring studies should not only include one of the environmental spheres. Thus, integrated environmental pollution assessment studies conducted in the biosphere, lithosphere, and atmosphere promote the “whole system” approach. In this study, the aim was to determine the pollution in the atmosphere, soil, and plants by taking advantage of the high pollution accumulation characteristics of the mosses. Prevailing wind has the potential to distribute pollutants emitted into the air throughout its path. With this regard, soil, mosses, and atmospheric deposition samples were collected in Çanakkale, Turkey, in two seasons. Concentrations of selected elements were measured by Inductively Coupled Plasma-Mass Spectrometry. The enrichment factor of the selected elements in the soil, moss, and deposition samples was calculated. The highest enrichments were found for Lead in atmospheric deposition, Arsenic in soil, and Mercury in moss samples. Cobalt and chromium accumulated more in mosses than in soil. Elevated arsenic levels found in the samples can pose a great risk for public health and agriculture. The study result showed that the elemental composition of the samples was influenced by the enhanced air plume dispersion of anthropogenic pollution sources along the Northeast-Southwest directions due to wind characteristics in the province. As expected, strong correlations were found among the moss, soil, and atmospheric deposition samples indicating the vital interactions between the environmental components.Öğe Development of melatonin-embedded PLGA-PEG6000 nanofiber biomaterial, and investigation of the effects on abdominal adhesion formation(SAGE Publications Ltd, 2024) Gökçe, Oruç Numan; Aykora, Damla; Danışman, Merve; Demir, Ufuk; Aydeğer, Cemre; Süner, Salih Can; Oral, Ayhan; Karaboğa, İhsan; Uzun, MetehanAbdominal adhesions are still among the most common postsurgical peritoneal inflammation-related complications. Adhesion-related disorders are still highly costly and prevalent due to advances in surgical techniques, treatment methods, and various drugs. The present study aimed to investigate the effects of Poly (D,L-lactide-co-glycolide) (PLGA)- polyethylene glycol (PEG6000) Nanofiber + Melatonin on the abdominal adhesion model in rats. For this purpose, PLGA-PEG6000 Nanofiber + Melatonin matrix was fabricated and implanted in an experimental abdominal adhesion model in rats. Our study consisted of an in vitro and an in vivo part. The degradation and release profile of the matrix and Melatonin (Mel) embedded matrix was performed in vitro. In vivo, the procedure was carried out with 18 Wistar male rats. Rats were divided into three groups as follows: Sham, Matrix, and Mel + Matrix, respectively. Consequent to degradation and release profiling in vitro, an experimental adhesion model was created and fabricated pure matrix (2 × 2 cm2), and matrix (2 × 2 cm2, 0.25 mg melatonin/per matrix embedded) was applied to injury area in related groups. Intra-abdominal adhesion scores were determined on post-op 21st day, under general anesthesia. Following, cecum, peritoneal tissue, and adhesive bands were harvested. Macroscopic analysis (severity of adhesion formation), Hematoxlyn&Eosin and Masson’s Trichrome staining (for the examination of the levels of infiltration of inflammatory cells, fibrosis, and neovascularization) were performed for the evaluation of the effects of Mel embedded and pure matrix Our results indicated that PLGA-PEG6000 Nanofiber + Melatonin matrix was degraded completely in rats abdominal cavity and significantly reduced adhesion formation compared to other groups macroscopically (p < 0.05). On the other hand, the histopathological analysis indicated that the fabricated matrix reduced inflammatory cell infiltration, fibrosis, and neovascularization levels.Öğe Major Bioactive Prenylated Flavonoids from Humulus lupulus L., Their Applications in Human Diseases and Structure-Activity Relationships (SAR) - A Review(Tabriz University of Medical Sciences, 2024) Cömert Önder, Ferah; Kalın, Sevil; Şahin, Nebahat; Davutlar, Gülce; Abusharkh, Khaled A.N.; Maraba, Özlem; Ay, Mehmet; Hal, Rabia SelinaIn recent years, the incidence of cancers, inflammatory diseases, Alzheimer's disease, glucose metabolism disorder and diabetes has increased alarmingly which demands more research into the discovery of new drug candidates to treat these human diseases. Main phytochemicals from Humulus lupulus L. (hops) have been demonstrated to have positive impacts on human health, and prenylated flavonoids are one of the major groups of bioactive phytochemicals found in this plant. Thus, this review summarizes the role of major prenylated components in hops in human diseases including cancer, inflammation and viral infections. In silico studies of prenylated bioactive compounds against various drug targets such as histone deactylases (HDACs), sirtuins (SIRTs), and acetylcholinesterase (AChE), and the molecular molecular interactions between protein and ligand have also been included. Furthermore, the structure-activity relationships (SAR) studies on these compounds are highlighted. This review concludes that the prenylated phytochemicals from H. lupulus L., including xanthohumol (XN), isoxanthohumol (IXN), 8-prenylnaringenin (8-PN) and 6-prenylnaringenin (6-PN), have promising roles in human health and may contribute to new drug discovery and development.Öğe Efficacy of one-hour negative pressure wound therapy and magnetic field energy in wound healing(MA Healthcare Ltd, 2024) Özer, Aysel Y.; Keskin, Alknur; Kelestemur, Taha; Ersavas, Cenk; Günal, Mehmet Y.; Aytürk, NilüferObjective: Wound healing is an important aspect of health but needs further research to identify the effects and interactions of different treatment approaches on healing. The aims of this study were to investigate the effectiveness of one-hour negative pressure wound therapy (NPWT) and compare histological differences between one-hour NPWT and magnetic field energy (MFE) in rats on early-stage wound healing, wound size and angiogenesis. Method: Standardised wounds were created on Wistar rats that were allocated and divided into NPWT, MFE and control groups. Both treatments were applied for 1 hour/day for 10 days. Wound size, histological changes and wound area blood flow were assessed. Results: The wound size of all groups was similar on days 0, 2 and 10. The MFE group's wound size was smaller than the NPWT group on days 4, 6 and 8 (p<0.05). Development of the granulation tissue in both the one-hour NPWT and MFE groups was greater than in the control group. Additionally, the inflammatory phase was shorter, and wounds entered the proliferative stage faster in the MFE group than both of the other groups. Conclusion: Treatment with MFE may be more effective in terms of early stage wound closure and angiogenesis. On the other hand, the NPWT group's wound area blood flow was significantly greater than the other two groups. MFE is superior to one-hour NPWT in terms of wound area and angiogenesis. Furthermore, it is worthwhile to note that one-hour NPWT increases bloodflow in the wound area, which stimulates healing. Keywords: angiogenesis; magnetic field; negative pressure wound therapy; wound; wound care; wound dressing; wound healing.Öğe Development of BDNF/NGF/IKVAV Peptide Modified and Gold Nanoparticle Conductive PCL/PLGA Nerve Guidance Conduit for Regeneration of the Rat Spinal Cord Injury(John Wiley and Sons Inc, 2024) Özçiçek, İlyas; Ayşit, Neşe; Balçıkanlı, Zeynep; Ulaş Aytürk, Nilufer; Aydeğer, Asel; Baydaş, GülsenaSpinal cord injuries are very common worldwide, leading to permanent nerve function loss with devastating effects in the affected patients. The challenges and inadequate results in the current clinical treatments are leading scientists to innovative neural regenerative research. Advances in nanoscience and neural tissue engineering have opened new avenues for spinal cord injury (SCI) treatment. In order for designed nerve guidance conduit (NGC) to be functionally useful, it must have ideal scaffold properties and topographic features that promote the linear orientation of damaged axons. In this study, it is aimed to develop channeled polycaprolactone (PCL)/Poly-D,L-lactic-co-glycolic acid (PLGA) hybrid film scaffolds, modify their surfaces by IKVAV pentapeptide/gold nanoparticles (AuNPs) or polypyrrole (PPy) and investigate the behavior of motor neurons on the designed scaffold surfaces in vitro under static/bioreactor conditions. Their potential to promote neural regeneration after implantation into the rat SCI by shaping the film scaffolds modified with neural factors into a tubular form is also examined. It is shown that channeled groups decorated with AuNPs highly promote neurite orientation under bioreactor conditions and also the developed optimal NGC (PCL/PLGA G1-IKVAV/BDNF/NGF-AuNP50) highly regenerates SCI. The results indicate that the designed scaffold can be an ideal candidate for spinal cord regeneration.Öğe Identification of apple varieties using hybrid transfer learning and multi-level feature extraction(Springer Science and Business Media Deutschland GmbH, 2023) Kılıçarslan, Serhat; Dönmez, Emrah; Kılıçarslan, SabireThe process of identifying apple varieties holds pivotal importance in pomology and agricultural science. This intricate task not only aids growers in optimizing orchard management, but also profoundly impacts consumers and the apple industry as a whole. Selecting the right apple varieties tailored to specific environmental conditions and market demands is instrumental for the sustainability and economic viability of apple cultivation. Accurate apple variety identification further contributes to maintaining product quality and ensuring consumer satisfaction. Traditional identification methods, however, are susceptible to human error given the vast diversity of apple cultivars. In response, the integration of advanced technologies, including image processing and machine learning, has emerged as a promising approach to enhance accuracy and efficiency in apple variety identification, benefitting both the agricultural and commercial sectors. The classification of apple types involved feature extraction using three methods: MobileNetV2, EfficientNetV2B0, and a combination of GLCM and Color-Space algorithms from apple images. Machine learning models were then built to classify apple varieties, utilizing various algorithms such as support vector machine (SVM), k-nearest neighbors (Knn), random subspace (RSS), and random forest. In the case of "EfficientNetV2B0 + GLCM + Color-Space" and utilizing the ReliefF feature selection method, the random forest algorithm attains peak performance with an accuracy, precision, recall, and F-score all registering an impressive 98.33%.Öğe Nitro Compounds Inhibit Breast Cancer Cell Proliferation, Migration, and Colony Formation: Molecular Docking, Molecular Dynamics Simulations and Pharmacological Properties(John Wiley and Sons Inc, 2023) Cömert Önder, Ferah; Şahin, Nebahat; Davutlar, Gülce; Önder, Alper; Ay, MehmetIn this study, the concept of combined in vitro and in silico studies were utilized by using some synthesized nitro bearing compounds. The anticancer activities of the studied compounds were performed by using colony formation analysis, cell cytotoxicity, and migration. Nitro group containing two compounds were analyzed using Hoechst staining to indicate the morphological changes on the nuclei of cancer cells under fluorescence microscopy. Scanning electron microscopy (SEM) analysis was performed with N,N-dibutyl-nitro-substituted compound. Nitro containing anticancer agents were shown the inhibition at 1.5 μM and 2 μM concentrations, and nuclear apoptosis was detected. In addition, cell-to-cell interaction on MDA-MB-231 cells was broken and observed morphologic changes following the treatment with N,N-dibutyl-nitro-substituted compound at the effective doses. In general, the other nitro compounds showed cell cytotoxicity at 5 μM, 10 μM, and 20 μM. Two hits as anticancer agents were determined as potential interleukin-1 receptor-associated kinase 1 (IRAK1) and interleukin-1 receptor-associated kinase 4 (IRAK4) inhibitor candidates. Molecular docking and molecular dynamics (MD) simulations studies will provide that the binding patterns with specific residues such as Met265, Tyr284 of the IRAK family members, and these will contribute to further in vitro and in vivo studies for targeted breast cancer therapy.Öğe A Chemically Induced Experimental Colitis Model with a Simple Combination of Acetic Acid and Trinitrobenzene Sulphonic Acid(Turkish Society of Gastroenterology, 2023) Cinpolat, Havva Yasemin; Buğdaycı, Güler; Şengül, Neriman; Astarcı, Hesna MüzeyyenBackground: It was aimed to induce a new experimental colitis model by using acetic acid and trinitrobenzene sulphonic acid together and to investigate the severity of inflammation biochemically and histopathologically in comparison with other models. Methods: Fifty-six Wistar albino male rats were randomly divided into 4 groups as control, acetic acid, trinitrobenzene sulphonic acid, and combined groups, and the animals were sacrificed following the induction of colitis on the third day and on the seventh day. The serum amyloid A and myeloperoxidase were tested in plasma samples, and the tumor necrosis factor-alpha, interleukin 33, and ST2 were assayed in colon tissue samples with enzyme-linked immunosorbent assay in addition to histopathological examination. Results: There were statistically significant differences between the combined and the control groups both on the third day and on the seventh day in all parameters. There was no difference between the acetic acid group on the seventh day and the control groups in biochemical parameters. Conclusions: The acetic acid model forms acute colitis. The combined model is found to be more successful in forming inflammation when compared to other models.Öğe Healing effects of L-carnitine on experimental colon anastomosis wound(Marmara University, 2023) Kandaş, Emel; Edremitlioğlu, Mustafa; Demir, Ufuk; Erbil, Güven; Şehitoğlu, Muşerref HilalObjective: The purpose of this study is to examine the effects of L-carnitine on healing of experimental colon anastomosis injury in early and late period.Materials and Methods: Forty female Wistar-Albino rats were used in this study. The rats were divided into 4 groups (CONT-3, CONT-7, CARN-3, and CARN-7). Injury healing was evaluated for CONT-3 group on the 3rd day and for CONT-7 group on the 7th day following the anastomosis. Following the operation, CARN-3 and CARN-7 groups were intraperitoneally administered with 100 mg/ kg/day L-carnitine and injury healing was evaluated on the 3rd and 7th days. Injury strength, histological evaluation and antioxidant enzyme activities and oxidant damage were determined in tissue samples of anastomosis area.Results: Bursting pressure levels and histological scoring values of CARN-3 group were found to be higher than the CONT-3 group (p<0.05). Antioxidant enzyme activities were found to be high in groups which were administered with L-carnitine, and oxidant damage was found to be significantly low in CARN-7 group (p<0.05).Conclusion: It was seen that L-carnitine speeds up the injury healing process and increases the injury strength and antioxidant capacity in early period. Increase in antioxidant enzyme activities was observed to be continued in late period as well.Öğe EFFECTS OF DIFFERENT DOSES OF SHIITAKE MUSHROOM (LENTINUS EDODES) ON SPERM PARAMETERS AND TESTICULAR TISSUE IN RATS: AN EXPERIMENTAL STUDY(Pakistan Agricultural Scientists Forum, 2023) Büyük, Başak; Demir, Neslihan; Eroğlu, Hüseyin Avni; Öztopuz, Rahime ÖzlemLentinus edodes has been cultivated and used as food and medicine in far eastern countries.The present study aimed to reveal whether L.edodes that is used for different purposes has a toxic effect on the male genital system and to investigate its relationship with male infertility by showing its effects on sperm parameters. Twenty-four male Wistar rats were randomly divided into three groups as Control (C), Low-dose (LD) and High-dose (HD). Rats in Control were administered saline physiological once a day for 7 days. The LD and HD groups received 100 and 400mg/kg of L.edodes extract, respectively, once daily for 7 days. At the end of 7 days of administration of treatments, semen analysis, micronucleus analysis, gene expression levels and testicular Johnsen scoring was performed. Comparisons between groups without normal distribution were analyzed using Kruskal-Wallis variance analysis and those between groups with normal distribution were analyzed using one-way analysis of variance, followed by Tukey's test. P values of <0.05 were considered statistically significant. It is demonstrated that both low and high doses of extract significantly reduced spermatogenesis in seminiferous tubules found in the testicles of rats. In addition, high-dose administration resulted in decreased total sperm counts, whereas the progressive and non-progressive sperm motility counts were significantly decreased in both experimental groups. The gene expression levels of Casp-3 and TNF-alpha were increased in both the LD and HD groups compared with that in the C. Oral administration of low and high doses of L.edodes led to reduced spermatogenesis in the testicles, tissue damage to the testicle and decreased motile and total sperm counts.Öğe The Effects of Thiopental on Cold Ischemic Injury in Renal Transplantation(Medical University of Plovdiv, 2023) Büyük, Başak; Adalı, Yasemen; Karakoç, Ebru; Eroğlu, Hüseyin Avni; Aydeğer, CemreIntroduction: One of the most important factors influencing post-transplant success in kidney transplantation is preserving the viability of the organ from removal to transfer into the recipient. Aim: This study aimed to reduce the energy requirement with thiopental doses administered before organ transplantation, and to increase the organ viability by minimizing the tissue damage during the cold ischemia process. Materials and methods: Twenty female Wistar albino rats were divided into two groups: control group (group C), and thiopental group (group T). In group C, a midline incision was performed, and the renal artery was isolated under ketamine and xylazine anesthesia. A standard organ storage solution (cooled to +4°C) was used for kidney perfusion. Nephrectomy was applied, and the removed kidneys were placed into +4°C standard organ storage solution and stored at +4°C for 12 hours. Animals in group T were subjected to the procedures explained above under 85 mg/kg thiopental sodium anesthesia. After 12-hour storage, samples from the kidney tissues were fixed in 10% neutral buffered formalin. Histopathological evaluation and apoptosis detection via TUNEL method were performed. Results: Tubular necrosis was more extensive in group C compared with that in group T and this difference was statistically significant. Similarly, vacuolization was widely observed in group C, and this increase was also statistically significant. For the ‘dilatation of Bowman’s space’ parameter, a significant decrease was observed in group T compared with group C. When the apoptotic index values of both groups were examined, it was seen that they were lower in group T than those in group C. This result was statistically significant. Conclusions: These data suggest that thiopental provides protection to the kidney tissue during the cold storage process. Thiopental has been shown to decrease the number of apoptotic cells in the kidney tissue when administered to the donor before organ transplantation, increasing the organ viability.Öğe Current evidence on the use of direct oral anticoagulants in patients with myeloproliferative neoplasm: a systematic review(Taylor and Francis Ltd., 2023) Baysal, Mehmet; Bayrak, Meltem; Eskazan, Ahmet EmreIntroduction: Thromboembolic events in myeloproliferative neoplasms (MPNs) are one of the most important causes of mortality and morbidity, in which vitamin K antagonists (VKAs) have been used mostly. Recently, direct oral anticoagulants (DOACs) are used in venous thromboembolism (VTE) and cancer-associated thrombosis (CAT). With the adoption of data from CAT and VTE, the usage of DOACs in MPNs is increasing. Areas covered: In this paper, we performed a systematic review to the current literature regarding the usage of DOACs in MPNs. Eleven studies involving 944 patients were included. The reasons for initiating DOACs were secondary prophylaxis for thrombosis (arterial or venous) and atrial fibrillation (AF) in 562 and 382 patients, respectively. A total of 84 (8.9%) recurrent thrombotic (arterial or venous) events recorded. Forty-six (8.1%) events occurred in the thrombosis group (arterial or venous) and 38 (9.9%) events occurred in patients with AF. Expert opinion: Ease of management and patient comfort should be regarded as benefits of DOACs compared to VKAs. However, it would be appropriate to bring an individualized approach until we obtain high-quality data with prospectively designed studies involving more patients and longer follow-up time concerning the use of DOACs in patients with MPNs.Öğe Evaluation of Serum Creatinine Levels with Reference Change Value in Patients Receiving Colistin Treatment(Oxford University Press, 2023) Cinpolat, Havva Yasemin; Alkan, Sevil; Altınışık, Hatice Betül; Ülker Çakır, Dilek; Oğuzman, HamdiObjective In this study, we aimed to evaluate the serum creatinine (SCr) levels with the reference change value (RCV) in patients receiving colistin treatment. Methods We retrospectively recorded the SCr levels of 47 patients receiving colistin treatment before treatment and on days 3 and 7 after treatment. RCV was calculated with the asymmetrical RCV formula (Z = 1.64, P < .05). Percent (%) increase in the SCr results of the patients was compared with RCV and values exceeding RCV were regarded as statistically significant. Results The RCV was calculated as 15.6% for SCr. Compared with pretreatment values, SCr value on day 3 was 32/47 and on day 7 it was 36/47; as these results exceeded RCV, they were considered statistically significant. Conclusion Use of RCV in the interpretation of results between serial measurements will provide a more rapid and sensitive method when making decisions.
