Relationship of Polymorphisms and Haplotype in Klotho Gene with Hypertension Risk

Abstract: Klotho gene is linked to promoting healthy aging and longevity. With age, serum Klotho levels decrease, resulting in age-related disorders. Polymorphisms contribute to a range of risk factors associated with disease onset, disease response, and therapeutic adverse effects. Klotho gene polymorphisms are linked to cardiovascular illness, although previous studies did not evaluate their relationship with hypertension. In this context, polymorphisms in the KL gene that disrupt gene expression or cause regulatory proteins to bind to the promoter region may trigger hypertension. So, the aim of our study investigated how two non-synonymous SNPs (rs9536314 and rs9527025) at the Klotho gene are connected to high blood pressure. The study was a case-control consisting of 226 hypertensive and 126 healthy individuals. All individuals were genotyped by real-time PCR. KL rs9536314 and rs9527025 polymorphisms were evaluated for hypertension risk using allele/genotype counting, OR-value, logistic regression, and haplotype analysis. As a results, hypertensive patients with recessive genotypes had twice the risk as the general population. KL rs9527025 was not a significant risk factor for dominant, recessive, and additive-modelled hypertension. In contrast, individuals with KL rs9536314 had a higher risk of hypertension in the recessive model. In conclusion: KL-rs9536314T → G would greatly increase the risk of hypertension in the recessive model. © Pleiades Publishing, Inc. 2025.