Lisansüstü Eğitim Enstitüsü Diğer Yayınlar Koleksiyonu

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https://hdl.handle.net/20.500.12428/3870

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Çanakkale İlinin Farklı Ekolojilerinde Yetiştirilen Bozcaada Çavuşu (Vitis vinifera L.) Üzüm Çeşidinde Pomolojik Özelliklerin Saptanması
(Çanakkale Onsekiz Mart Üniversitesi, 2024) Dardeniz, Alper; Dinçkal, Serdar
Bu araştırma, Çanakkale ilinin farklı ekolojilerinde yetiştirilen ‘Bozcaada Çavuşu’ üzüm çeşidinde pomolojik özelliklerin saptanması amacıyla, 2023 yılı vejetasyon döneminde yürütülmüştür. Araştırmada, Çanakkale ili Bozcaada ilçesinde farklı mevkilerdeki 4 adet ve Bayramiç ilçesi Çavuşlu köyünde farklı mevkilerdeki 2 adet ‘Bozcaada Çavuşu’ üzüm çeşidi bağından salkım örnekleri alınmıştır. Araştırma, tesadüf parselleri deneme deseninde 3 tekerrürlü olarak planlanmış ve her tekerrürde 3’er adet omcaya yer verilmiştir. Bozcaada 1; en uzun boylu, en sık, en ağır salkımların elde edildiği, salkımlardaki toplam tane ve normal tane sayısının ile çekirdek sayısının en fazla olduğu, en kısa tane en, boy ve ağırlığı ile en düşük pH’a sahip bağ olmuştur. Bozcaada 2; en az partenokarpik taneye ve en yüksek SÇKM ve pH’a sahip bağ olarak belirlenmiştir. Bozcaada 3; en dar, en hafif, en az normal tane sayısına sahip gevşek yapılı ve en olgun salkımların elde edildiği bağ olmuştur. Bayramiç 1; en geniş salkımların, en fazla partenokarpik ve toplam kalın kabuklu tanenin alındığı, en düşük SÇKM ile olgunluk indisinin elde edildiği ve en parlak ve yeşil renkli tanelerin alındığı bağ olmuştur. Bayramiç 2; en kısa salkımların, en enli, uzun ve en ağır tanelerin elde edildiği bağ olarak tespit edilmiştir.
Farklı Biçim Uygulamalarına Bazı Sorgum Sudanotu Melezi Çeşitlerinin Agronomik Özellikleri ile Otunun Enerji Değerlerindeki Değişimlerin İncelenmesi
(Çanakkale Onsekiz Mart Üniversitesi, 2024) Özsüer, Münir Sadi; Alatürk, Fırat
Bu çalışma sorgum sudanotu melezi (SSM) çeşitlerinde (Nutri Honey ve Nutrima) farklı hasat uygulamalarına bağlı olarak toprak üstü ve toprak altı biomass üretimi ile yaprak ve sapların enerji içeriklerinin belirlenmesi amacıyla 2020-2021 yıllarında Çanakkale Onsekiz Mart Üniversitesi Ziraat Fakültesi araştırma alanında yürütülmüştür. Bölünmüş parseller deneme desenine göre 4 tekrarlamalı olarak kurulan araştırmada ana parselleri çeşitler (Nutri Honey ve Nutrima), alt parselleri ise biçim yükseklikleri (bitki boyu 30, 60, 90, 120, 150 cm olduğunda ve fizyolojik olum döneminde hasat) oluşturmuştur. Araştırmada bitkilerle ilgili olarak yeşil ot verimi, yaprak, sap ve salkım oranları, kök miktarı, yaprak ve sap kısımlarına ait TSBM, ME, NE ve SE değerleri incelenmiştir. Toplam yeşil ot verimleri biçimdeki bitki boyunun artışına bağlı olarak artmıştır. Nutri Honey çeşidinin toplam yeşil ot üretimi (7323.0 kg da-1) Nutrima’ya (7019.3 kg da-1) göre daha yüksek olmuştur. Bitkide büyümeye bağlı olarak yaprak oranları düşerken, sap ve salkım oranlarında artışlar olmuştur. Bitkilerde boy uzaması ile kök üretimi arasında olumlu ilişki olduğu ve boy uzunluğu arttıkça üretilen kök miktarının da arttığı tespit edilmiştir. Yaprakların TSBM içerikleri saplara göre %4.82, ME içerikleri %5.63, NE içerikleri %5.43 ve SE içerikleri %4.84 daha yüksek olmuştur. Yapılan çalışmanın sonunda benzer ekolojilerde kaba yem kaynağı olarak her iki sorgum sudanotu melezi çeşitlerinin yetiştirilmesi ve 150 cm bitki boyuna ulaştıktan sonra hasat edilerek yetiştirme döneminde iki kere verim alınması önerilmektedir.
UNDERSTANDING AND SUPPORTING SPECIAL EDUCATION: EXPERIENCES OF SCHOOL ADMINISTRATORS
(Centro Univ La Salle-Rj, 2024) Çil, Gürkan; Kırat, Emel; Aydın, Sevcan; Güven, Bülent
This study aims to determine school administrators' attitudes toward special education, their level of knowledge, their collaboration experiences, and their in-service training needs. The study group of the research, which was conducted with a case study based on qualitative research methods, consisted of eight school administrators who served in public schools in the academic year of 2022-2023. Five themes were identified by analyzing the data obtained through the interview technique. The results of the study revealed that the school administrators described individuals with special needs as individuals who differ from their peers in certain areas and need special attention and education. The school administrators considered themselves inadequate in the field of special education. In the collaboration theme, they emphasized the significance of family collaboration but indicated that they were not supported as needed. Moreover, the administrators needed in-service trainings and seminars. Consequently, the role of administrators in special education has a direct impact on the education and development of students with special needs. They should have a broad accumulation of knowledge, exhibit strong leadership skills and act with an empathetic approach. In this sense, it is expected that the organization of in-service trainings and seminars will enhance the quality of special education services by improving the knowledge and skills of administrators.
Neuroanatomical Correlates of Memory Strategies in Rats
(Wiley, 2025) Balcı, Aysu; Cankurtaran, Buse Pınar; Akyel, Aybüke; Arıkan, Kutluk Bilge; Ünal, Bengi; Ünal, Çağrı Temuçin
Navigation incorporates a continuum of strategies, where the allocentric strategy relies on relationships between environmental landmarks resulting in a cognitive map, and the egocentric strategy revolves around the body position and stimulus response chains with the body as a reference. Although multiple brain regions contribute to navigation, the hippocampus dominates allocentric navigation, whereas the striatum is key for egocentric navigation. Neuromodulators, such as dopamine and acetylcholine, regulate both the hippocampus and striatum to influence behavior, yet their influence on navigational strategy has not been determined. Interindividual differences in strategy preference are known to exist. Building on these pre-existing interindividual differences, this study explored the neuroanatomical underpinnings on navigational strategy variations in rats through a dual-solution T-maze and immunocytochemistry. Surprisingly, interindividual variations eluded explanation through the density of cholinergic neurons supplying acetylcholine to the hippocampus and striatum. Similarly, the soma morphologies of these neurons exhibited no discernible differences. Dopaminergic cell densities in the ventral tegmental area (VTA), projecting to the hippocampus, and substantia nigra pars compacta (SNpc), projecting to the striatum, failed to account for individual variations as well. Nevertheless, allocentric rats displayed higher VTA/SNpc dopamine neuron fusiformity indexes, potentially contributing to computational distinctions underlying interindividual variations in navigation strategies. This study delves into potential explanations and charts promising avenues for future research. A graphical abstract summarizing the main findings of this study is provided.
Gene expression responses unveil the negative effects of Olive Mill Wastewater on Gammarus komareki (Amphipoda: Gammaridae)
(Springer, 2025) Yılmaz, Sevdan; Odabaşı, Deniz Anıl; Zilifli, Aytuğ; Banni, Mohammed; Abdel-Latif, Hany M. R.
Olive Mill Wastewater (OMW) is a major organic pollutant, particularly in Mediterranean countries with intensive olive oil production. In this study, the toxic effects of OMW were tested on a freshwater macroinvertebrate, Gammarus komareki. Animals were exposed to different concentrations of 1/5, 1/10, and 1/20 of the previously calculated 72-h LC50 (LC50 = 3.65%) and was applied as high (0.73%), medium (0.365%) and low (0.1825%) levels, respectively for 20 days. At exposure, female and male individuals from each group were sampled separately. Samples were analyzed for the expression levels of antioxidant defences (cat, gst, se-gpx, and mn-sod), reproduction (vitellogenin-like protein, vtg), respiration (hemocyanin, hc), antitoxic defences (heat shock protein 70, hsp70), and molting (molt-inhibiting hormone, mih) related genes. Results revealed that the expression levels of vtg gene in females exposed to low doses were increased significantly compared to other groups; however, its levels were decreased in group exposed to high doses compared to the control and other groups. The mRNA expression levels of mn-sod and se-gpx genes of males and female individuals exposed to low OMW doses increased significantly compared to other doses. The mRNA expression levels of gst gene increased in males and females exposed to moderate doses and only in males exposed to low doses compared to other groups. The mRNA expression levels of cat gene of females were similar in all groups, while their levels were higher in males exposed to OMW than in the control group. The expression values of hsp70 gene were significantly decreased in females and all males exposed to high doses of OMW. On the other hand, the mih gene expression in females was increased at low doses of OMW, while decreased in the groups exposed to high doses compared to the control group. In male individuals, the mih expression levels significantly increased in all experimental groups. The expression of the hc gene was decreased in male and female individuals exposed to all doses of OMW compared to the control group. To put it briefly, the great variations in the gene expression levels in groups exposed to OMW underlines the toxic effects of this effluent and its negative impacts on the aquatic organisms.
A Model System for Understanding Host-Pathogen Relationships Through In Vitro Host Mimicry
(Springer, 2025) Fidanoğlu, Baran Taylan; Keresteci, Nadir; Özkılınç, Hilal
In vitro host mimicry provides a method to study host-pathogen interactions without constraints of time, space, or host presence. Monilinia fructicola and M. laxa, pathogens causing brown rot disease, have a narrow host range including some pome and stone fruits. Building on previous in vitro mimicry of the peach host and M. fructicola, this paper confirms that various hosts of both fungal species can be mimicked in vitro, offering insights into host preferences. This study developed mimic media for peach, plum, cherry, and apple hosts, evaluating pathogen preferences based on mycelial growth and the expression levels of two effector genes: cutinase 1 (CUT1) and polygalacturonase 1 (PG1). Isolates with different virulence levels were used to assess aggressiveness effects. Effector genes were significantly expressed in host-mimicking media compared to standard potato dextrose agar. Moreover, host preference with the virulence level. The high-virulent M. fructicola isolates favored peach-based medium, while low-virulent isolates preferred plum-based medium. All M. laxa isolates showed the highest growth on peach-based media and the slowest on cherry-based media. This study showed Monilinia host-pathogen relationships in vitro, and the research approach can be used as a model for necrotrophic fungal pathosystems.
Exploring the perspectives of university students on post-COVID-19 rental housing demands: a case study of Çanakkale, Türkiye
(Springer, 2025) Uşaklı, Metin; Yücebaş, Sait Can; Genç, Levent
This study focused on utilizing Machine Learning (ML) to examine the housing preferences of students. Employing a survey method, the study utilized decision tree, a widely favored ML approach, to present findings. The analysis focused on the post-COVID-19 rental housing preferences of students and their impact on rental prices. Furthermore, the research identified the number of rooms as a crucial factor for male students, particularly for first-year students, with gender becoming significant for second-, third-, and fourth-year students in Barbaros neighborhood. A noteworthy post-COVID-19 trend was the observation that students, in general, preferred communal living arrangements, sharing rental costs. Additionally, the study found that under different circumstances, male students were more inclined to lease housing in & Ccedil;anakkale province compared to their female counterparts.
A comparative study on removal of boron via pervaporation and vacuum membrane distillation using zirconium metal-organic framework-loaded poly(lactic acid) membrane
(Springer Heidelberg, 2025) Nigiz, Filiz Uğur; Tan, Burcu; Bektaş, Tijen Ennil; Karakoca, Betül
Boron mineral is very important for the life. However, exceeding the standards of boron minerals, especially in water to be used as domestic water, causes health and environmental problems. The commercial method used to separate boron minerals from water is reverse osmosis. In recent years, promising results have been obtained with the membrane distillation (MD) method. However, another method that is as effective as this method is pervaporation (PV). The most important component that affects performance in both methods is the membranes. In this study, zirconium-based metal organic framework (MOF) material was synthesized and added to the polylactic acid (PLA) membrane and boron was removed by pervaporation and membrane distillation methods. While the selective layered asymmetric membrane was prepared for pervaporation, porous membranes were prepared for membrane distillation. The effect of MOF additive on the morphology, mechanical strength, and separation properties of the membrane was investigated. Additionally, the effects of boron concentration and temperature on the separation performance in both methods were examined. As a result, the mechanical strength of membranes with MOF added increased significantly from 2.41 to 8.20 MPa. 99.9% boron removal was achieved in both methods. While the highest flux value was calculated as 8 kg/m2h in pervaporation at 6 ppm boron concentration, it was calculated as 11.33 kg/m2h in membrane distillation.
Prediction of newly synthesized heparin mimic's effects as heparanase inhibitor in cancer treatments via variational quantum neural networks
(Elsevier Sci Ltd, 2025) Kocabay, Samet; Acar, Erdi; Memiş, Samet; İçen Taşkın, Irmak; Sever, Meryem Rüveyda; Şener, Ramazan
Cancer remains a leading global cause of death, primarily driven by the uncontrolled proliferation of abnormal cells. Malignant tumors, such as carcinomas, originate from unchecked epithelial cell growth and produce growth factors like FGF and VEGF, which promote angiogenesis and tumor progression through heparanasemediated degradation of heparan-sulfate proteoglycans. Chitosan and its derivatives have shown promise in inhibiting tumor growth and metastasis. This study aims to investigate newly synthesized sulfated chitosan oligomers as heparin mimics to inhibit heparanase, evaluating their cytotoxic effects on SH-SY5Y, HCT116, A549, and MDA-MB-231 cancer cell lines. Moreover, it seeks to leverage a variational quantum neural network (VQNN) to predict and validate cytotoxicity outcomes, integrating quantum computing methods into evaluating novel anticancer compounds. The VQNN algorithm was applied to analyze the anticancer effects of sulfated chitosan oligomers. Cytotoxicity data from wet lab experiments validated the model's predictive performance. The VQNN model demonstrated strong predictive capabilities in evaluating anticancer compounds. Specifically, it achieved a mean absolute error (MAE) of 6.5844, indicating a similar trend to the experimental results. Additionally, the model obtained an R2 value of 0.6020, reflecting a moderate level of correlation between predicted and observed outcomes. The results underscore the potential of integrating quantum-based machine learning models into cancer research. The VQNN effectively predicted experimental outcomes, showcasing its utility in assessing novel anticancer compounds. This approach could speed up drug discovery by streamlining the identification and optimization of therapeutic candidates. Furthermore, the findings support the ongoing development of quantum computing techniques for tackling complex biological challenges, contributing to innovative cancer treatment strategies that target tumor growth and angiogenesis.
Fabrication and characterization electrospun clinoptilolite filled polylactic acid composite membrane: purification of multiple impurities from water
(Elsevier, 2025) Kahraman, Seniyecan; Katırcı, Ayşenur; Aytaç, Ayşe; Veli, Sevil; Nigiz, Filiz Uğur
In this study, electrospun clinoptilolite (Clp)- polylactic acid (PLA) nanocomposite membranes were produced and used for dyestuff (methylene blue, MB), oil (soybean oil), microplastic (Polyamide 66, PA66), and Linear Alkyl Benzene Sulfonate (LAS) rejection from simulated gray water. The membrane's physical, chemical, thermal, morphological, mechanical, and antimicrobial properties were investigated regarding Clp incorporation. According to the characterization results, the empirical porosity of the membrane was found to be between 79 % and 83 %. The PLA membrane's water uptake capacity, surface hydrophilicity, and mechanical strength were improved with Clp incorporation. All membranes show antimicrobial effects against both gram-positive and negative bacteria. According to the filtration test results, MB separation was above 90 %. The highest oil rejection was found to be 87.84 % using 4 wt% Clp loaded membrane. All membranes rejected 100 % of microplastic. 66.6 % of LAS rejection was achieved with 3 wt% Clp loaded membrane. All separation and characterization results show that Clp-doped PLA membranes have the potential to be used as a filtration membrane that can simultaneously separate all impurities from water.
Resistance evolution and local adaptation of Venturia inaequalis to old and new generation SDHI fungicides
(Elsevier Sci Ltd, 2025) Özkılınç, Hilal; Fidanoğlu, Baran Taylan; Öncel, Sibel; Kurtuluş, Ezgi; Kadıoğlu, İrem Ece
The use of succinate dehydrogenase inhibitor (SDHI) fungicides is widespread in controlling apple scab disease caused by Venturia inaequalis. However, resistance in populations develops and new SDHIs are generated commercially, thus evaluating this arms-race in local populations is important for appropriate disease management. In this study, in vitro mycelial growth effects of five commercial fungicides, including old and new generation SDHIs on a large V. inaequalis population in T & uuml;rkiye were investigated. Overall, the entire population was resistant to Boscalid (Cantus (R)), and the most effective fungicides tested were Isopyrazam (REFLECT (R)) and Pydiflumetofen (Candidate fungicide, Miravis (R)). Although the results show the effectiveness of new generation SDHIs, remarkable differences in pathogen resistance responses were detected at local level. There were also differences in colony growth rates among isolates, but this was not associated with fungicide response traits. Furthermore, the intraspecies genetic diversities obtained from the sequence data of the four selected gene regions were depicted with the Neigbour_Joining (NJ) trees. There appeared to be no relationship between NJbased branching and fungicide response phenotypes. As a result of mating type determinationof the isolates with newly designed primers, 1:1 ratio indicated possible recombination in almost all local groups, with one exception. Additionally, detection of both mating type genes in some isolates was an interesting finding about the reproductive structure of the pathogen. This study, which is the first comprehensive study of V. inaequalis in T & uuml;rkiye, presents new findings about the pathogen from different perspectives.
Machinability of different Cu-Gr composites in milling: Performance parameters prediction via machine learning models
(Pergamon-Elsevier Science Ltd, 2025) Sap, Serhat; Acar, Erdi; Değirmenci, Ünal; Usca, Üsame Ali; Memiş, Samet; Şener, Ramazan
The machinability of copper-graphite (Cu-Gr) composites has gained significant attention due to their unique thermal, electrical, and mechanical properties. This study experimentally investigates the machinability performances (such as surface roughness, flank wear, cutting temperature, and energy consumption) of Cu-Gr hybrid composite materials during milling. It predicts these parameters with machine learning models. The study aims to contribute to sustainable and optimized manufacturing processes by analyzing the effects of different cutting parameters and cooling/lubrication conditions on this performance. Furthermore, advanced artificial intelligence-based models predict machining outcomes, providing a robust framework for process enhancement and industrial implementation. Although there are comprehensive studies on the machining performances of metal matrix composites in the literature, there is limited information on Cu-Gr composites' mechanical and thermal behaviors in milling processes. To address this deficiency, a full factorial experimental plan was applied on six different Cu-Gr composites and the effects of different cutting speeds, feed rates and cooling/ lubrication environments (Dry, MQL, cryogenic LN2) on flank wear, surface roughness, cutting temperature and energy consumption were analyzed. The materials used in the study were prepared by mixing graphite and hard phases (Al2O3 and Cr3C2) in specific proportions, and these composites were compared in terms of machinability. Afterward, the output parameters of the experimental results are predicted by employing the well-known machine learning models and the experimental results. The results manifested that Gradient-Boosted Decision Tree Regression performs better than the other ten machine learning models in predicting machinability parameters. Finally, this study highlights potential areas for future research and provides a practical guide for optimizing CuGr composites in manufacturing processes and achieving sustainability goals. It has engineering value in efficiency, cost reduction, and developing environmentally friendly applications, especially for the automotive, aerospace, and energy sectors.
Genotoxic and cytotoxic effects of polystyrene nanoplastics on human lymphocytes: A comprehensive analysis
(Elsevier, 2025) Berber, Ahmet Ali; Akıncı Kenanoğlu, Nihan; Demir, Şefika Nur; Aksoy, Hüseyin
A growing amount of plastic waste is finding its way into natural ecosystems as a result of the widespread usage of plastics in modern society. These wastes degrade physically and biologically over time, transforming into microplastics (MPs) and nanoplastics (NPs). MPs and NPs emissions from the terrestrial environment then mix with rivers and eventually the seas, forming garbage. The cytotoxic and genotoxic effects of 50 nm polystyrene nanoplastics (PsNP) on human lymphocytes were assessed using the in vitro mitotic index (MI), micronucleus (MN), and comet assays. Both 24 and 48-h applications were performed for MI, and it was determined that 50 nm PsNP provided a statistically significant decrease in MI compared to the control at all concentrations and application times (except 0.001 and 0.1 mu g/mL at 24 h). According to the MN test results, the MN frequency increased significantly at all concentrations when compared to the negative control. In the comet test, a statistically significant increase of comet tail length was observed at 0.001, 10 and 100 mu g/mL concentration with 50 nm PsNP exposure. Tail moment also showed a statistically significant increase at the lowest concentration of 0.001 mu g/mL and the highest concentration of 1, 10, 100 mu g/mL compared to the negative control. All test results show that PsNP has both genotoxic and cytotoxic potential.
Synthesis, Hydrolytic Degradation Behavior, and Surface Properties of Poly(alkyl glycolide)-Polyglycolide Copolymers
(Amer Chemical Soc, 2025) Cantürk Bamyacı, Mehtap; Çetin, Duygu; Cengiz, Candan; Belen, Sema Nur; Mert, Olcay; Cengiz, Uğur; Mert, Serap
Given the environmental impact of polymers on our daily lives, the development of biodegradable polymers is becoming increasingly critical. Poly(diisobutyl glycolide)-polyglycolide (PDIBG-PGA) and poly(diisopropyl glycolide)-polyglycolide (PDIPG-PGA) copolymers, which are structurally similar to polylactic-co-glycolic acid (PLGA) polyesters frequently used in the field of biomaterials, were synthesized via ring-opening polymerization (ROP) of glycolide with l-diisobutyl glycolide (l-DIBG) or l-diisopropyl glycolide (l-DIPG), respectively, in various molecular weights (M w GPC: 15.5-40.0 kDa) and in high yields (up to 85.0%). The wettability characteristics of biodegradable polymers are important not only in air but also for their behavior in underwater environments. PDIBG-PGA silica composites, due to their amphiphilic nature, exhibited water contact angles between 72 degrees and 85 degrees in air, unaffected by the increasing addition of hydrophilic silica nanoparticles. However, underwater-oil contact angles increased from 75 degrees to 165 degrees as a result of the higher silica nanoparticle content and enhanced surface roughness. When the silica content reached 30%, the surface demonstrated self-cleaning and oil-repellent properties underwater, attributed to the Cassie state, which trapped air within the surface's hierarchical roughness. Furthermore, the surface free energy (SFE) values of PDIBG-PGA and PDIPG-PGA copolymer films were evaluated using the Owens-Wendt method, which revealed an increasing underwater hexadecane contact angle as the polar component interactions increased. Differential scanning calorimetry analysis revealed that all synthesized copolymers were amorphous, and the glass transition temperatures (T g) increased with the increase in the molecular weight of the copolymers (for instance, M n GPC: 9560 g/mol -> T g: 25.1 degrees C vs M n GPC: 20,850 g/mol -> T g: 32.3 degrees C for PDIBG-PGA; M n GPC: 10,670 g/mol -> T g: 37.7 degrees C vs M n GPC: 23,360 g/mol -> T g: 42.3 degrees C for PDIPG-PGA). The molecular weight decreases of 88.3% and 76.5% and mass losses of 36.7% and 12.3% were observed for PDIBG-PGA and PDIPG-PGA copolymers after 8 weeks of hydrolytic degradation, respectively. The faster degradation of PDIBG-PGA (T g: 25.1 degrees C) than PDIPG-PGA (T g: 37.7 degrees C) may be attributed to the T g below the hydrolytic degradation temperature (37 degrees C) because of an increase in the mobility of PDIBG-PGA polymer chains, allowing water molecules to transfer more easily through the matrix.
Discovering the dynamics of peach fruit mycobiome throughout fruit development season by high-throughput sequencing
(Nature Portfolio, 2025) Öncel, Sibel; Özkılınç, Hilal
The mycobiome is comprised of a rich array of fungal species that compete for resources, and species diversity and prevalence exhibit a dynamic structure under the influence of many factors. While the host fruit develops, the prevalence and the arrangement of fungal species in this mycobiome also change, forming a dynamic microenvironment. In this study, fungal diversity on peach fruit surfaces at different developmental stages have been determined to better understand the changes in fungal diversity and disease occurrence by using metabarcoding of the full ITS region and processing the obtained high-throughput sequencing data with various bioinformatic analyses. It has been found that fungal diversity in early developmental stages is higher, and the diversity declines as the fruit matures, likely due to more prevalent fungal species establishing themselves on the surface as the fruit develops. Additionally, this research reveals that the prevalence of pathogens does not necessarily mean that disease will develop, as pathogenic species were found to be at higher prevalence percentages when compared to non-pathogenic species in healthy fruit samples. This study also identified the Monilinia polystroma species at a molecular level for the first time in T & uuml;rkiye; however, no symptomatic signals were recorded on the host. The study provides valuable data for mycobiome studies, while also highlighting its importance in optimizing sustainable disease management strategies.
Identification and inhibition of PIN1-NRF2 protein-protein interactions through computational and biophysical approaches
(Nature Portfolio, 2025) Özleyen, Adem; Duran, Gizem Nur; Dönmez, Serhat; Özbil, Mehmet; Doveston, Richard G.; Boyuneğmez Tümer, Tuğba
NRF2 is a transcription factor responsible for coordinating the expression of over a thousand cytoprotective genes. Although NRF2 is constitutively expressed, its stability is modulated by the redox-sensitive protein KEAP1 and other conditional binding partner regulators. The new era of NRF2 research has highlighted the cooperation between NRF2 and PIN1 in modifying its cytoprotective effect. Despite numerous studies, the understanding of the PIN1-NRF2 interaction remains limited. Herein, we described the binding interaction of PIN1 and three different 14-mer long phospho-peptides mimicking NRF2 protein using computer-based, biophysical, and biochemical approaches. According to our computational analyses, the residues positioned in the WW domain of PIN1 (Ser16, Arg17, Ser18, Tyr23, Ser32, Gln33, and Trp34) were found to be crucial for PIN1-NRF2 interactions. Biophysical FP assays were used to verify the computational prediction. The data demonstrated that Pintide, a peptide predominantly interacting with the PIN1 WW-domain, led to a significant reduction in the binding affinity of the NRF2 mimicking peptides. Moreover, we evaluated the impact of known PIN1 inhibitors (juglone, KPT-6566, and EGCG) on the PIN1-NRF2 interaction. Among the inhibitors, KPT-6566 showed the most potent inhibitory effect on PIN1-NRF2 interaction within an IC50 range of 0.3-1.4 mu M. Furthermore, our mass spectrometry analyses showed that KPT-6566 appeared to covalently modify PIN1 via conjugate addition, rather than disulfide exchange of the sulfonyl-acetate moiety. Altogether, such inhibitors would also be highly valuable molecular probes for further investigation of PIN1 regulation of NRF2 in the cellular context and potentially pave the way for drug molecules that specifically inhibit the cytoprotective effects of NRF2 in cancer.
Production of natural zeolite-filled recycled PVDF filters and their application for gray water treatment
(Royal Soc Chemistry, 2025) Katırcı, Ayşenur; Kahraman, Seniyecan; Uğur Nigiz, Filiz
In this study, clinoptilolite (Clp)-doped poly(vinylidene fluoride) (PVDF) membranes (1-4 wt%) were prepared using an electrospinning method. Further, filtration tests on the simulated gray water components were investigated. Methylene blue (MB), linear alkyl benzene sulfonate (LAS), oil (soybean oil), and microplastic (MP) filtration were performed. MB filtration with the PVDF membrane resulted in over 99% of MB rejection. Oil rejection with the PVDF membrane without Clp was observed to be 95%, while the addition of Clp increased the oil rejection to over 99%. It was observed that LAS rejection increased as the Clp content increased. MP rejection using PVDF-based membranes was 100%. Considering all the test results, the membrane containing 3 wt% Clp showed the best performance, and the process parameters and rejection efficiencies were determined through experimental optimization. Synthetic gray water analyses included the chemical oxygen demand (COD), pH, conductivity, and total dissolved solids (TDS). COD rejection was 63.1%, while turbidity rejection was 97.2%.
In silico molecular docking and in vitro analysis of atomoxetine
(Taylor & Francis Ltd, 2025) Bolat, Nurullah; Hız-Çelikliyurt, Merve Meliha; Akıncı, Erhan; Akkuş, Gülsüm; Günay, Melih; Korkmaz, Şükrü Alperen
Although atomoxetine, a selective norepinephrine reuptake inhibitor, is widely used in the treatment of attention-deficit/hyperactivity disorder (ADHD), there is limited data on its cytogenetic effects. This study aimed to investigate the cytotoxicity and genotoxicity of atomoxetine in vivo and silico. Chromosome aberration and micronucleus assays were used to analyze the genotoxic effect of atomoxetine in human peripheral blood lymphocytes under culture conditions. The mitotic index was assessed for cytotoxic potential. For the docking analysis, DNA receptor (1BNA) was prepared with ChimeraX, and the Atomoxetine molecule was optimized by Avogadro2.0 software. In silico molecular docking analysis was carried out utilizing SwissDock online platform. The results obtained were visualized using ChimeraX and Pymol software. Atomoxetine doses of 9.6 mu g/mL (equal to about 1.2 mg/kg as a maintenance dose), 14.4 mu g/mL (equal about to 1.8 mg/kg as the highest dose systematically tested), 48.0 mu g/mL (equal about to 6 mg/kg as five times the maintenance dose) and 96.0 mu g/mL (equal about to 12 mg/kg as ten times the maintenance dose) were analyzed. The findings clearly indicate that atomoxetine has no genotoxic effect at the therapeutic dose. However, we observed genotoxic effects at 48.0 and 96.0 mu g/mL doses. No strong binding affinity occurs in silico analyses. As one of the initial inquiries into the in silico and in vivo appraisal of atomoxetine's genotoxic impacts, the research has established that atomoxetine does not significantly affect the frequency of chromosomal damage or micronucleus formation. Genotoxic effects should be kept in mind at doses above clinical practice.
Exploring the moderating role of university environmental performance in shaping students' environmental awareness and behaviors
(Emerald Group Publishing Ltd, 2025) Eroğlu, Erdal; Aydemir Dev, Mine; Dalgın, Kübra
PurposeThe purpose of this study is to examine the moderating role of university environmental performance in the relationship between students' environmental awareness and behavior, with a particular focus on the imperative for universities to adopt comprehensive sustainability frameworks. Design/methodology/approachUsing a quantitative research methodology, the study administered a survey to 775 undergraduate students from diverse academic departments at a public university. The survey aimed to assess students' environmental awareness, behaviors and perceptions of university environmental performance. The Mann-Whitney U test, Wilcoxon signed-rank test and Friedman test were applied, and a simple moderation analysis was performed.FindingsThe results indicate that environmental awareness significantly influences students' environmental behaviors. Crucially, the perception of environmental performance moderates the relationship between environmental awareness and behavior. The relationship between environmental awareness and environmental behaviors becomes stronger when environmental performance increases.Research limitations/implicationsWhile the scope of this research is limited to a single institution, potentially limiting the generalizability of the findings, future studies could extend this investigation to multiple universities to confirm and expand upon these results.Originality/valueThis research contributes to the existing body of literature by emphasizing the critical role universities play in cultivating students' environmental awareness and behaviors, and it underscores the importance of institutional environmental performance in promoting sustainable practices within academic environments. Although previous research has explored environmental awareness and behavior, this study offers a unique contribution as it establishes a connection between the awareness-behavior gap and environmental performance.
Behaviors of dark energy candidates in the Ruban universe
(World Scientific Publ Co Pte Ltd, 2025) Engin, Melike; Aktaş, Can
In this study, we investigated quintessence and tachyon field dark energy (DE) models for the inhomogeneous and anisotropic Ruban universe in f(R,T) gravitation theory. We utilized the Hubble parameter in the field equations as beta root t+alpha for the solutions. Since DE candidates are classified according to the p/rho values of the EoS parameter omega, we obtained the p and rho solutions for each DE candidate and analyzed the scalar field (SF) and scalar potential solution. We also talked about the model's physical characteristics and parameters with the help of a various of graphics for redshift z and cosmic time t. Additionally, the statefinder parameters, which are essential tools for distinguishing various dark energy models, have been explored.

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