IMPROVED METHODS OF CANE SAMPLES STAINING DESTINED FOR MICROSCOPIC EXAMINATION AND IMAGING

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dc.contributor.authorKuzucu, F. C.
dc.contributor.authorGokbayrak, Z.
dc.contributor.authorEngin, H.
dc.date.accessioned2025-01-27T21:05:41Z
dc.date.available2025-01-27T21:05:41Z
dc.date.issued2024
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn this study, staining methods were used to improve the effective visualization of grapevine cane sections under a microscope using 'ring light'. The stains applied to the sections included Aniline Blue, Safranin O, Toluidine Blue O, Bromophenol Blue, Basic Fuchsin, Methyl Green, Giemsa Stain, Carmine and Fast Green FCF. The natural color differences observed between the tissues were similar. Therefore, staining was proven to be the most useful tool to increase the contrast in grapevine cane tissues. Different stains reacted with different cell types and components and revealed their unique color properties. In addition to single use of stains, Carmine + Methyl Green, Fast Green FCF + Bromophenol Blue and Safranin O + Bromophenol Blue were utilized as double staining. Among these applications, staining with Aniline Blue, Bromophenol Blue, Fast Green FCF, Safranin O, and Toluidine Blue O showed effective results on cell and tissue levels. In addition, successful results were obtained from double stains. Double staining was recommended for examining grapevine cane sections under a microscope. 'Ring light' has been developed to improve the coloration and sharpness on the surface of the stained sections under the microscope and during photography. The ring light offers the opportunity to illuminate the entire sample by using white light, yellow light and both light intensities in different
dc.identifier.doi10.15666/aeer/2206_54675481
dc.identifier.issn1589-1623
dc.identifier.issn1785-0037
dc.identifier.scopus2-s2.0-85211324808
dc.identifier.scopusqualityQ3
dc.identifier.urihttps://doi.org/10.15666/aeer/2206_54675481
dc.identifier.urihttps://hdl.handle.net/20.500.12428/27761
dc.identifier.wosWOS:001347942600001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherAloki Applied Ecological Research And Forensic Inst Ltd
dc.relation.ispartofApplied Ecology and Environmental Research
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectsections
dc.subjectgrapevine
dc.subjectstains
dc.subjecttissue
dc.subjectcane
dc.subjectstem
dc.titleIMPROVED METHODS OF CANE SAMPLES STAINING DESTINED FOR MICROSCOPIC EXAMINATION AND IMAGING
dc.typeArticle

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