IMPROVED METHODS OF CANE SAMPLES STAINING DESTINED FOR MICROSCOPIC EXAMINATION AND IMAGING
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Tarih
2024
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Aloki Applied Ecological Research And Forensic Inst Ltd
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
In this study, staining methods were used to improve the effective visualization of grapevine cane sections under a microscope using 'ring light'. The stains applied to the sections included Aniline Blue, Safranin O, Toluidine Blue O, Bromophenol Blue, Basic Fuchsin, Methyl Green, Giemsa Stain, Carmine and Fast Green FCF. The natural color differences observed between the tissues were similar. Therefore, staining was proven to be the most useful tool to increase the contrast in grapevine cane tissues. Different stains reacted with different cell types and components and revealed their unique color properties. In addition to single use of stains, Carmine + Methyl Green, Fast Green FCF + Bromophenol Blue and Safranin O + Bromophenol Blue were utilized as double staining. Among these applications, staining with Aniline Blue, Bromophenol Blue, Fast Green FCF, Safranin O, and Toluidine Blue O showed effective results on cell and tissue levels. In addition, successful results were obtained from double stains. Double staining was recommended for examining grapevine cane sections under a microscope. 'Ring light' has been developed to improve the coloration and sharpness on the surface of the stained sections under the microscope and during photography. The ring light offers the opportunity to illuminate the entire sample by using white light, yellow light and both light intensities in different
Açıklama
Anahtar Kelimeler
sections, grapevine, stains, tissue, cane, stem
Kaynak
Applied Ecology and Environmental Research
WoS Q Değeri
N/A
Scopus Q Değeri
Q3
