Carbapenem resistance and biofilm formation status of Enterobacterales isolated from raw milk via molecular versus phenotypic methods

Basit Öğe Kaydı
dc.authorid0000-0002-1613-5917en_US
dc.authorid0000-0002-6451-7354en_US
dc.authorid0000-0003-2146-8254en_US
dc.authorid0000-0003-1755-7705en_US
dc.authorid0000-0003-2946-5437en_US
dc.authorid0000-0001-6851-6474en_US
dc.authorscopusid57954947800en_US
dc.authorscopusid57223399184en_US
dc.authorscopusid58019014100en_US
dc.authorscopusid57372837700en_US
dc.authorscopusid56050706100en_US
dc.authorscopusid56050112300en_US
dc.authorwosidA-1824-2018en_US
dc.authorwosid-en_US
dc.authorwosidIVH-6501-2023en_US
dc.authorwosid-en_US
dc.authorwosidAAQ-6692-2021en_US
dc.authorwosidAAG-4281-2021en_US
dc.contributor.authorÖzdikmenli Tepeli, Seda
dc.contributor.authorTosun, Melike Nur
dc.contributor.authorTaylan Yalçın, Gizem
dc.contributor.authorKaya, Burcu
dc.contributor.authorİpek, Dilvin
dc.contributor.authorZorba, Nükhet Nilüfer
dc.date.accessioned2023-09-09T12:36:51Z
dc.date.available2023-09-09T12:36:51Z
dc.date.issued2023en_US
dc.departmentEnstitüler, Lisansüstü Eğitim Enstitüsü, Gıda Mühendisliği Ana Bilim Dalı
dc.departmentFakülteler, Mühendislik Fakültesi, Gıda Mühendisliği Bölümü
dc.departmentMeslek Yüksekokulları, Ezine Meslek Yüksekokulu, Gıda İşleme Bölümü
dc.departmentMeslek Yüksekokulları, Yenice Meslek Yüksekokulu, Veterinerlik Bölümü
dc.description.abstractAntibiotic resistance genes can easily be transferred between bacteria in the biofilm. In the dairy industry, many bacterial species forming biofilms on the surfaces of equipment are widely reported. The experiments reported in this research paper aimed to investigate the carbapenem resistance and biofilm formation properties of Enterobacterales isolates which are spoilage microorganisms obtained from raw milk. In addition, the study determined that whether there was a relationship between the biofilm formation ability or the protein spectra of these isolates. In this study, ninety-two Enterobacterales isolates collected from 173 raw milk samples were investigated. Initially, the isolates were identified as Citrobacter braakii (n = 18), Citrobacter freundii (n = 12), Enterobacter asburiae (n = 1), Enterobacter cloacae (n = 3), Escherichia coli (n = 10), Hafnia alvei (n = 18), Klebsiella oxytoca (n = 1), Serratia fonticola (n = 24), Serratia liquefaciens (n = 4), and Serratia marcescens (n = 1) using MALDI-TOF MS. As a result, carbapenem resistance was determined in 6.5% of the isolates by CIM test, MHT, and the disk diffusion methods, but none of them had blaOXA-48, blaKPC, blaNDM-1, blaOXA23, blaOXA-58, blaOXA-51, blaVIM, and blaIMP genes. This may be due to the effect of other resistance mechanisms such as porin loss or increased flow pump activity. Furthermore, biofilm formation (weak and moderate) was detected in 97.8% of the Enterobacterales isolates. The mass spectra of the moderate biofilm producer isolate of Serratia spp. and the mass spectra of the weak biofilm producers of E.coli presented similarities.en_US
dc.identifier.citationTepeli, S. O., Çevik, Y. N., Tosun, M. N., Yalçın, G. T., Kaya, B., İpek, D., … Zorba, N. N. (2023). Carbapenem resistance and biofilm formation status of Enterobacterales isolated from raw milk via molecular versus phenotypic methods. Antonie van Leeuwenhoek, 116(1), 67–80. https://doi.org/10.1007/s10482-022-01799-5en_US
dc.identifier.doi10.1007/s10482-022-01799-5
dc.identifier.endpage80en_US
dc.identifier.issn0003-6072
dc.identifier.issn1572-9699
dc.identifier.issue1en_US
dc.identifier.pmid36525156
dc.identifier.scopus2-s2.0-85144158707
dc.identifier.startpage67en_US
dc.identifier.urihttps://doi.org/10.1007/s10482-022-01799-5
dc.identifier.urihttps://hdl.handle.net/20.500.12428/4554
dc.identifier.volume116en_US
dc.identifier.wosWOS:000920862300001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorÖzdikmenli Tepeli, Seda
dc.institutionauthorTosun, Melike Nur
dc.institutionauthorTaylan Yalçın, Gizem
dc.institutionauthorKaya, Burcu
dc.language.isoen
dc.publisherSpringer Science and Business Media Deutschland GmbHen_US
dc.relation.ispartofAntonie van Leeuwenhoek, International Journal of General and Molecular Microbiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAntibiotic resistanceen_US
dc.subjectBiofilmen_US
dc.subjectCarbapenemen_US
dc.subjectEnterobacteralesen_US
dc.subjectMALDI-TOF MSen_US
dc.subjectMastitisen_US
dc.subjectRaw milken_US
dc.titleCarbapenem resistance and biofilm formation status of Enterobacterales isolated from raw milk via molecular versus phenotypic methods
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
Seda_Ozdikmenli_Tepeli_Makale.pdf
Boyut:
1.3 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Araştırma Makalesi
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Ezine Meslek Yüksekokulu Koleksiyonu
Gıda Mühendisliği Bölümü Koleksiyonu
Lisansüstü Eğitim Enstitüsü Diğer Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
Daha fazla yükle