Investigating the cytotoxicity and genotoxicity of Vortioxetine with in vivo and in silico methods

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dc.authorid0000-0001-6760-1458
dc.authorid0000-0003-4482-7352
dc.authorid0000-0003-4303-9717
dc.authorid0000-0003-0336-3010
dc.contributor.authorCicekliyurt, Meliha Merve
dc.contributor.authorInan, Pinar
dc.contributor.authorGunay, Melih
dc.contributor.authorAkkus, Gulsum
dc.contributor.authorCicekliyurt, Altug
dc.date.accessioned2026-02-03T12:02:29Z
dc.date.available2026-02-03T12:02:29Z
dc.date.issued2025
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractVortioxetine is a multimodal antidepressant with reported benefits on cognitive functioning, social functioning, and fatigue, however its potential genotoxic risks have not been adequately studied. Therefore, this study aims to investigate the cytotoxicity and genotoxicity of vortioxetine in vivo and in silico. Genotoxic effects were assessed using Chromosome aberration (CA) and micronucleus (MN) assays in cultured human peripheral blood lymphocyte. Cytotoxicity was evaluated through Mitotic index (MI), and DNA interaction was analysed by UV titration and agarose gel electrophoresis. In silico analyses were performed with Attraction Cavities and AutoDock Vina methods. Experimental results demonstrate that vortioxetine binds to Calf Thymus DNA (CT-DNA) through intercalative interactions and cleaves pBR322 DNA in the presence of hydrogen peroxide. DNA binding studies indicated that groove binding is the effective interaction between vortioxetine and CT-DNA (Kb: 6.25 x 105 M- 1). It was also supported by molecular docking results, where binding affinities of vortioxetine and escitalopram were - 7.29 and - 7.69 kcal/mol for Attracting Cavities and - 6.01 and - 6.57 kcal/mol for AutoDock Vina. When comparing vortioxetine to escitalopram, both drugs were found to be potentially genotoxic. These findings suggest a potential genotoxic risk with prolonged use and provide valuable insight for clinicians in evaluating long-term safety.
dc.description.sponsorshipCanakkale Onsekiz Mart University
dc.description.sponsorshipScientific Research Coordination Unit [THD-2022-3867]
dc.description.sponsorshipFinancial support and sponsorship: Canakkale Onsekiz Mart University supported this study by The Scientific Research Coordination Unit, Project number: THD-2022-3867.
dc.identifier.doi10.1038/s41598-025-14866-4
dc.identifier.issn2045-2322
dc.identifier.issue1
dc.identifier.pmid41022849
dc.identifier.scopus2-s2.0-105017706267
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1038/s41598-025-14866-4
dc.identifier.urihttps://hdl.handle.net/20.500.12428/34778
dc.identifier.volume15
dc.identifier.wosWOS:001586165500034
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherNature Portfolio
dc.relation.ispartofScientific Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20260130
dc.subjectVortioxetine
dc.subjectCytotoxicity
dc.subjectGenotoxicity
dc.subjectDepression
dc.subjectMolecular docking
dc.titleInvestigating the cytotoxicity and genotoxicity of Vortioxetine with in vivo and in silico methods
dc.typeArticle

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