Variation of NPY and AGRP MRNA expression in Syrian hamsters according to feeding times

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dc.authoridGündüz, Bülent / 0000-0003-0497-8287
dc.authoridİnan, Pınar / 0000-0001-6760-1458
dc.contributor.authorİnan, Pınar
dc.contributor.authorGündüz, Bülent
dc.date.accessioned2025-01-27T20:47:26Z
dc.date.available2025-01-27T20:47:26Z
dc.date.issued2024
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThe purpose of this study was to determine the impact of feeding time on NPY/AgRP protein and mRNA expression in the brain during prenatal and postnatal periods. Feeding at different times of the day during pregnancy may have lasting effects on the hypothalamic circuitry in offspring energy homeostasis. In the experimental design, adult female hamsters were randomly assigned to receive three different feeding conditions: ad libitum, night-time feeding and day-time feeding groups. After the lactation period, feeding regimens were continued for the offspring born from mothers who were given nutritional regimens, until they were 30 days old. RT-PCR for NPY/AgRP mRNA expression and ELISA analysis for protein levels were performed on hypothalamus tissues of hamsters at 10, 20 and 30 days of postnatal stage. There was no difference between the groups in terms of the daily measured food consumption of the offspring's. Body weights were significantly decreased in both night-time and day-time feeding groups compared to the ad libitum group (p<0.05). The highest increase in mRNA expression of NPY/AgRP was seen in the samples taken at 10. day of lactation in the night-time and day-time feeding groups of the offspring treated with three different feeding regimens. When the feeding regimes were compared, it was observed that the protein and mRNA expression of both NPY and AGRP increased the most in the offspring groups in which the feeding was restricted night -time only. Early stages of development have shown that maternal factors have significantly affected the offspring NPY and AgRP mRNA expression and protein levels. These results show that the metabolic regulation of energy balance may change with maternal factors during the very early stages of development.
dc.description.sponsorshipCanakkale Onsekiz Mart University Scientific Research Projects Commission [BAP 2020/3196]; Council of Higher Education (YOK) [YOK 100/2000]
dc.description.sponsorshipThis project was supported by Canakkale Onsekiz Mart University Scientific Research Projects Commission with the project number BAP 2020/3196.P & imath;nar & Idot;NAN, who took part in this study, was supported by the Council of Higher Education (YOK) throughout the PhD. Program to work in the field of Human Brain and Neuroscience within the scope of YOK 100/2000 PhD. Scholarship Program.
dc.identifier.doi10.14744/sigma.2023.00072
dc.identifier.endpage1343
dc.identifier.issn1304-7205
dc.identifier.issn1304-7191
dc.identifier.issue5
dc.identifier.scopus2-s2.0-85206142422
dc.identifier.scopusqualityQ3
dc.identifier.startpage1336
dc.identifier.urihttps://doi.org/10.14744/sigma.2023.00072
dc.identifier.urihttps://hdl.handle.net/20.500.12428/24900
dc.identifier.volume42
dc.identifier.wosWOS:001339231600002
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherYildiz Technical Univ
dc.relation.ispartofSigma Journal of Engineering and Natural Sciences-Sigma Mühendislik Ve Fen Bilimleri Dergisi
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectELISA
dc.subjectMaternal Transfer
dc.subjectMesocricetus Auratus
dc.subjectNPY/ AgRP Neurons
dc.subjectRT-PCR
dc.titleVariation of NPY and AGRP MRNA expression in Syrian hamsters according to feeding times
dc.typeArticle

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