A novel acetylcholinesterase inhibition based colorimetric biosensor for the detection of paraoxon ethyl using CUPRAC reagent as chromogenic oxidant

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dc.authorid0000-0003-0320-3551en_US
dc.authorid-en_US
dc.authorid0000-0002-2980-6871en_US
dc.authorscopusid57197830377en_US
dc.authorscopusid58502069600en_US
dc.authorscopusid6507953466en_US
dc.authorwosid-en_US
dc.authorwosid-en_US
dc.authorwosidW-4565-2017en_US
dc.contributor.authorAyaz, Selen
dc.contributor.authorUlucay, Sude
dc.contributor.authorUzer, Ayşem
dc.contributor.authorDilgin, Yusuf
dc.contributor.authorApak, Reşat
dc.date.accessioned2024-02-20T07:37:33Z
dc.date.available2024-02-20T07:37:33Z
dc.date.issued2024en_US
dc.departmentFakülteler, Fen Fakültesi, Kimya Bölümü
dc.departmentFakülteler, Mühendislik Fakültesi, Kimya Mühendisliği Bölümü
dc.description.abstractA novel colorimetric biosensor for the sensitive and selective detection of an organophosphate pesticide, paraoxon ethyl (POE), was developed based on its inhibitory effect on the acetylcholine esterase (AChE) enzyme. The bis-neocuproine copper (II) complex ([Cu(Nc)2]2+) known as the CUPRAC reagent, was used as a chromogenic oxidant in the AChE inhibition-based biosensors for the first time. To initiate the biosensor, an enzymatic reaction takes place between AChE and its substrate acetylthiocholine (ATCh). Then, enzymatically produced thiocholine (TCh) reacts with the light blue [Cu(Nc)2]2+ complex, resulting in the oxidation of TCh to its disulfide form. On the other hand, [Cu(Nc)2]2+ reduces to a yellow-orange cuprous complex ([Cu(Nc)2]+) which gives maximum absorbance at 450 nm. However, the absorbance of [Cu(Nc)2]+ proportionally decreased with the addition of POE because the inhibition of AChE by the organophosphate pesticide reduced the amount of TCh that would give a colorimetric reaction with the CUPRAC reagent. Based on this strategy, the linear response range of a colorimetric biosensor was found to be between 0.15 and 1.25 μM with a detection limit of 0.045 μM. The fabricated biosensor enabled the selective determination of POE in the presence of some other pesticides and metal ions. The recovery results between 92% and 104% were obtained from water and soil samples spiked with POE, indicating that the determination of POE in real water and soil samples can be performed with this simple, accurate, sensitive, and low-cost colorimetric biosensor.en_US
dc.identifier.citationAyaz, S., Uluçay, S., Üzer, A., Dilgin, Y. & Apak, R. (2024). A novel acetylcholinesterase inhibition based colorimetric biosensor for the detection of paraoxon ethyl using CUPRAC reagent as chromogenic oxidant. Talanta, 266. https://doi.org/10.1016/j.talanta.2023.124962en_US
dc.identifier.doi10.1016/j.talanta.2023.124962
dc.identifier.issn0039-9140
dc.identifier.issn1873-3573
dc.identifier.pmid37499364
dc.identifier.scopus2-s2.0-85165536373
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2023.124962
dc.identifier.urihttps://hdl.handle.net/20.500.12428/5660
dc.identifier.volume266en_US
dc.identifier.wosWOS:001047177300001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorAyaz, Selen
dc.institutionauthorUluçay, Sude
dc.institutionauthorDilgin, Yusuf
dc.language.isoen
dc.publisherElsevier B.V.en_US
dc.relation.ispartofTalantaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.relation.tubitakinfo:eu-repo/grantAgreement/TUBITAK/SOBAG/120Z963
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAcetylcholine esteraseen_US
dc.subjectColorimetric biosensoren_US
dc.subjectCUPRAC reagenten_US
dc.subjectEnzyme inhibitionen_US
dc.subjectParaoxon ethylen_US
dc.titleA novel acetylcholinesterase inhibition based colorimetric biosensor for the detection of paraoxon ethyl using CUPRAC reagent as chromogenic oxidant
dc.typeArticle

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