A novel acetylcholinesterase inhibition based colorimetric biosensor for the detection of paraoxon ethyl using CUPRAC reagent as chromogenic oxidant
Yükleniyor...
Tarih
2024
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier B.V.
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A novel colorimetric biosensor for the sensitive and selective detection of an organophosphate pesticide, paraoxon ethyl (POE), was developed based on its inhibitory effect on the acetylcholine esterase (AChE) enzyme. The bis-neocuproine copper (II) complex ([Cu(Nc)2]2+) known as the CUPRAC reagent, was used as a chromogenic oxidant in the AChE inhibition-based biosensors for the first time. To initiate the biosensor, an enzymatic reaction takes place between AChE and its substrate acetylthiocholine (ATCh). Then, enzymatically produced thiocholine (TCh) reacts with the light blue [Cu(Nc)2]2+ complex, resulting in the oxidation of TCh to its disulfide form. On the other hand, [Cu(Nc)2]2+ reduces to a yellow-orange cuprous complex ([Cu(Nc)2]+) which gives maximum absorbance at 450 nm. However, the absorbance of [Cu(Nc)2]+ proportionally decreased with the addition of POE because the inhibition of AChE by the organophosphate pesticide reduced the amount of TCh that would give a colorimetric reaction with the CUPRAC reagent. Based on this strategy, the linear response range of a colorimetric biosensor was found to be between 0.15 and 1.25 μM with a detection limit of 0.045 μM. The fabricated biosensor enabled the selective determination of POE in the presence of some other pesticides and metal ions. The recovery results between 92% and 104% were obtained from water and soil samples spiked with POE, indicating that the determination of POE in real water and soil samples can be performed with this simple, accurate, sensitive, and low-cost colorimetric biosensor.
Açıklama
Anahtar Kelimeler
Acetylcholine esterase, Colorimetric biosensor, CUPRAC reagent, Enzyme inhibition, Paraoxon ethyl
Kaynak
Talanta
WoS Q Değeri
Q1
Scopus Q Değeri
Cilt
266
Sayı
Künye
Ayaz, S., Uluçay, S., Üzer, A., Dilgin, Y. & Apak, R. (2024). A novel acetylcholinesterase inhibition based colorimetric biosensor for the detection of paraoxon ethyl using CUPRAC reagent as chromogenic oxidant. Talanta, 266. https://doi.org/10.1016/j.talanta.2023.124962
