Secondary metabolite production in callus culture of Verbascum scamandri Murb.

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dc.contributor.authorCambaz, Ebru
dc.contributor.authorCorduk, Nursen
dc.date.accessioned2025-01-27T20:22:28Z
dc.date.available2025-01-27T20:22:28Z
dc.date.issued2023
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractVerbascum scamandri Murb. known as Kazdagi Mullein is an endemic species in Turkiye and is classified as an endangered (EN) species. The aim of this study is to establish an efficient callus culture for V. scamandri and analyze the amounts of verbascoside, luteolin, and aucubin metabolites of calli samples. Leaf explants were cultured on MS medium with cytokinin (BAP, Kin, 0, 0.5, 1, 2, 3 mg/L) and auxin (NAA, 2,4-D, 0, 0.1, 0.5, 1 mg/L), 1 g/L PVP, 3% sucrose, and 0.7% agar for callus induction. Callus tissue in MS with 2 mg/L Kin, 0.5 mg/L Kin + 0.5 mg/L 2,4-D, 2 mg/L Kin + 0.5 mg/L 2,4-D, and 3 mg/L Kin + 0.5 mg/L 2,4-D was proliferated in MS basal medium containing PGR at the same concentrations and combinations as the callus induction media. Verbascoside, luteolin, and aucubin were quantified in leaf samples of the in vivo collected plants, leaf samples of in vitro growing plants, and calli using HPLC-DAD. According to the results, the verbascoside content in the leaf of collected plants was 7.03 mg/g, luteolin was 0.66 mg/g, and aucubin was 2.99 mg/g..e leaf of in vitro plants had 1.62 mg/g verbascoside, 0.18 mg/g luteolin, and 1.32 mg/g aucubin. Whereas, the maximum content of secondary metabolites in the callus samples was observed 13.77 mg/g verbascoside in MS medium with 2 mg/L Kin, 0.51 mg/g luteolin in MS medium with 2 mg/L Kin + 0.5 mg/L 2,4-D, and 9.32 mg/g aucubin in 0.5 mg/L Kin + 0.5 mg/L 2,4-D.
dc.description.sponsorshipScientific Research Project Coordination Unit of Canakkale Onsekiz Mart University [FYL-2021-3732]
dc.description.sponsorshipThis study was financially supported by the Scientific Research Project Coordination Unit of Canakkale Onsekiz Mart University, Turkiye (FYL-2021-3732).
dc.identifier.doi10.5586/asbp/165894
dc.identifier.issn0001-6977
dc.identifier.issn2083-9480
dc.identifier.scopus2-s2.0-85167677636
dc.identifier.scopusqualityQ3
dc.identifier.urihttps://doi.org/10.5586/asbp/165894
dc.identifier.urihttps://hdl.handle.net/20.500.12428/21900
dc.identifier.volume92
dc.identifier.wosWOS:001066577000001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherPolskie Towarzystwo Botaniczne
dc.relation.ispartofActa Societatis Botanicorum Poloniae
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectcallus
dc.subjectendemic
dc.subjectin vitro
dc.subjectmullein
dc.subjectScrophulariaceae
dc.subjectsecondary metabolites
dc.subjectVerbascum scamandri
dc.titleSecondary metabolite production in callus culture of Verbascum scamandri Murb.
dc.typeArticle

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