Transposable elements in genomic architecture of Monilinia fungal phytopathogens and TE-driven DMI-resistance adaptation

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dc.authoridDurak, Muhammed Raşit / 0000-0001-8792-2304
dc.authoridÖzkılınç, Hilal / 0000-0003-0791-975X
dc.contributor.authorDurak, Muhammed Raşit
dc.contributor.authorÖzkılınç, Hilal
dc.date.accessioned2025-05-29T02:57:38Z
dc.date.available2025-05-29T02:57:38Z
dc.date.issued2025
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractBackgroundFungicide resistance poses a significant challenge to plant disease management and influences the evolutionary dynamics of fungal pathogens. Besides being important phytopathogens, Monilinia species have become a model for discovering many fundamental questions related to fungal pathosystems. In this study, DMI-propiconazole sensitivity was investigated in view of transposable element (TE) dynamics in M. fructicola and M. laxa.ResultsPropiconazole-sensitivity of 109 M. fructicola and 20 M. laxa isolates from different regions of T & uuml;rkiye was assessed. Comprehensive TE identification within the species revealed that Class I elements were predominant, and TEs constituted approximately 9% of the genome for both M. fructicola and M. laxa, with a total of 15,327 and 10,710 TEs, respectively. An experimental evolution plan was developed for Monilinia that allows observing phenotypic and genotypic changes over successive generations under controlled selection pressures. Dynamic changes in TE content were discovered throughout the experimental evolution of M. fructicola under propiconazole pressure. With a net change of 187 TEs, the evolved strain showed an expansion of TE sequences, whereas different TE classes displayed diverse patterns of increase/decrease. Additionally, the presence of a nested TE upstream of the CYP51 gene was observed in less-sensitive M. fructicola isolates but absent in highly-sensitive ones. Gene expressions of CYP51 differed significantly between TE-containing and TE-lacking isolates, strongly supporting the contribution of this TE to fungicide resistance.ConclusionThis study establishes a critical link between TEs and DMI fungicide resistance by associating a nested TE with reduced sensitivity to propiconazole. We introduce an innovative experimental evolution framework for studying genomic changes under selective pressure and provide a comprehensive characterization of Monilinia TEs. These findings significantly advance our understanding of molecular resistance mechanisms in fungal pathogens, offering insights for more effective disease management.
dc.description.sponsorshipScientific Research Project Coordination Unit of Canakkale Onsekiz Mart University [FHD-2022-4083]; Scientific Research Project Coordination Unit of Canakkale Onsekiz Mart University [FYL-2021-3681]
dc.description.sponsorshipThis work was supported by the Scientific Research Project Coordination Unit of Canakkale Onsekiz Mart University (Project No: FHD-2022-4083) for the investigation of CYP51 gene expressions associated with propiconazole resistance. The part of the study including DMI-propiconazole in vitro sensitivity assays and TE characterizations was supported by the Scientific Research Project Coordination Unit of Canakkale Onsekiz Mart University (Project No: FYL-2021-3681).
dc.identifier.doi10.1186/s13100-025-00343-2
dc.identifier.issn1759-8753
dc.identifier.issue1
dc.identifier.pmid40055830
dc.identifier.scopus2-s2.0-86000322439
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1186/s13100-025-00343-2
dc.identifier.urihttps://hdl.handle.net/20.500.12428/30109
dc.identifier.volume16
dc.identifier.wosWOS:001439365700001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherBmc
dc.relation.ispartofMobile Dna
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250529
dc.subjectTransposable elements
dc.subjectMonilinia species
dc.subjectFungicide resistance
dc.subjectExperimental evolution
dc.titleTransposable elements in genomic architecture of Monilinia fungal phytopathogens and TE-driven DMI-resistance adaptation
dc.typeArticle

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