Humoral immune response of Galleria mellonella after mono- and co-injection with Hypericum perforatum extract and Candida albicans

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dc.authoridCakaloglu, Cagla/0000-0001-9446-6239
dc.authoridTURGUT GENC, Tulay/0000-0001-5074-3572
dc.authoridGUNAY, Melih/0000-0003-0336-3010
dc.authoridKAYA, Serhat/0000-0002-0984-2824
dc.contributor.authorGenc, Tulay Turgut
dc.contributor.authorKaya, Serhat
dc.contributor.authorGunay, Melih
dc.contributor.authorCakaloglu, Cagla
dc.date.accessioned2025-01-27T20:51:50Z
dc.date.available2025-01-27T20:51:50Z
dc.date.issued2024
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractGalleria mellonella is used as a model organism to study the innate immune response of insects. In this study, the humoral immune response was assessed by examining phenoloxidase activity, fungal burden, and the expression of phenoloxidase and antimicrobial peptide genes at different time point following separate and combined injections of Hypericum perforatum extract and a nonlethal dose of Candida albicans. The administration of a plant extract at low doses increased phenoloxidase activity, while higher doses had no effect. Similarly, co-injection of a low dose of the extract with the pathogen allowed half of the yeast cells to survive after 24 h. Co-injection of plant extract with the pathogen decreased the phenoloxidase activity at the end of 4 h compared to C. albicans mono-injection. The phenoloxidase gene expressions was reduced in all experimental conditions with respect to the control. When plant extracts and the pathogen were administered together, gallerimycin and hemolin gene expressions were considerably higher compared to mono-injections of plant extracts and the pathogen. The results of this study reveal that gene activation and regulatory mechanisms may change for each immune gene, and that recognition and signaling pathways may differ depending on the involved immunoregulator.
dc.description.sponsorshipScientific Research Coordination Unit of Canakkale Onsekiz Mart University, Turkiye [FBA2021-2839]
dc.description.sponsorshipThe work is an outcome of research project number FBA2021-2839 financed by the Scientific Research Coordination Unit of Canakkale Onsekiz Mart University, Turkiye.
dc.identifier.doi10.1111/apm.13383
dc.identifier.endpage370
dc.identifier.issn0903-4641
dc.identifier.issn1600-0463
dc.identifier.issue5
dc.identifier.pmid38344892
dc.identifier.scopus2-s2.0-85185095033
dc.identifier.scopusqualityQ1
dc.identifier.startpage358
dc.identifier.urihttps://doi.org/10.1111/apm.13383
dc.identifier.urihttps://hdl.handle.net/20.500.12428/25536
dc.identifier.volume132
dc.identifier.wosWOS:001161313400001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofApmis
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectGreater wax moth
dc.subjectinsect immunity
dc.subjectgene expression
dc.subjectplant extract
dc.subjectpathogen
dc.titleHumoral immune response of Galleria mellonella after mono- and co-injection with Hypericum perforatum extract and Candida albicans
dc.typeArticle

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