An ultrasensitive and disposable electrochemical aptasensor for prostate-specific antigen (PSA) detection in real serum samples

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dc.authorid0000-0002-3735-1213en_US
dc.authorid0000-0003-4296-2657en_US
dc.authorid0000-0003-3042-1087en_US
dc.authorscopusid55838812200en_US
dc.authorscopusid57212611236en_US
dc.authorscopusid6506764918en_US
dc.authorwosid-en_US
dc.authorwosidHDN-2668-2022en_US
dc.authorwosidAAY-1439-2021en_US
dc.contributor.authorÖzyurt, Canan
dc.contributor.authorUludağ, İnci
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2023-08-21T08:17:38Z
dc.date.available2023-08-21T08:17:38Z
dc.date.issued2023en_US
dc.departmentFakülteler, Mühendislik Fakültesi, Biyomühendislik Bölümü
dc.departmentMeslek Yüksekokulları, Lapseki Meslek Yüksekokulu, Kimya ve Kimyasal İşleme Teknolojileri Bölümü
dc.description.abstractIn this study, we constructed a disposable indium tin oxide polyethylene terephthalate film (ITO-PET)-based electrochemical aptasensor for analyzing prostate-specific antigen (PSA), one of the most important biomarkers of prostate cancer. Because of their clinical importance, building PSA biosensing systems with high sensitivity and stability is essential. However, it still presents significant difficulties, such as low detection limits. We designed a platform to covalently bind the amino-terminated aptamer by modifying the ITO-PET surface with carboxyethylsilanetriol (CTES) to obtain a self-assembled monolayer (SAM). We also evaluated the potential for use in real human serum samples by investigating the optimal operating conditions and analytical performance characteristics of the developed biosensor. The design we present here exhibits excellent precision, with a limit of detection (LOD) as low as 8.74 fg/mL PSA. The broad linear detection range of the biosensor under optimal conditions was determined as 1.0-1500 fg/mL. The dissociation constant (Kd) for the aptamer was also calculated as 46.28 ± 5.63 nM by evaluating the impedimetric response as a function of PSA concentration. The aptasensor displayed considerable repeatability (1.3% RSD) and reproducibility (7.51% RSD) and good storage stability (98.34% of the initial activity for 8 weeks). Additionally, we demonstrated that the technique we developed was quite efficient in estimating the kinetics of aptamer-analyte interactions by determining the Kd and single-frequency impedance (SFI) data. In conclusion, we proposed a selective and sensitive biosensor with the potential for clinical application and superior performance in real serum samples.en_US
dc.identifier.citationÖzyurt, C., Uludağ, İ., & Sezgintürk, M. K. (2023). An ultrasensitive and disposable electrochemical aptasensor for prostate-specific antigen (PSA) detection in real serum samples. Analytical and Bioanalytical Chemistry, 415(6), 1123–1136. https://doi.org/10.1007/s00216-022-04309-8en_US
dc.identifier.doi10.1007/s00216-022-04309-8
dc.identifier.endpage1136en_US
dc.identifier.issn1618-2642
dc.identifier.issn1618-2650
dc.identifier.issue6en_US
dc.identifier.pmid36155829
dc.identifier.scopus2-s2.0-85138835241
dc.identifier.startpage1123en_US
dc.identifier.urihttps://doi.org/10.1007/s00216-022-04309-8
dc.identifier.urihttps://hdl.handle.net/20.500.12428/4504
dc.identifier.volume45en_US
dc.identifier.wosWOS:000859821100002
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorÖzyurt, Canan
dc.institutionauthorUludağ, İnci
dc.institutionauthorSezgintürk, Mustafa Kemal
dc.language.isoen
dc.publisherSpringer Science and Business Media Deutschland GmbHen_US
dc.relation.ispartofAnalytical and Bioanalytical Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAptameren_US
dc.subjectAptasensoren_US
dc.subjectBiosensoren_US
dc.subjectElectrochemical impedance spectroscopyen_US
dc.subjectProstate canceren_US
dc.subjectProstate-specific antigenen_US
dc.titleAn ultrasensitive and disposable electrochemical aptasensor for prostate-specific antigen (PSA) detection in real serum samples
dc.typeArticle

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