Biyomühendislik Bölümü Koleksiyonu

Bu koleksiyon için kalıcı URI

https://hdl.handle.net/20.500.12428/2631

Listeleniyor 1 - 20 / 155

A disposable biosensing system for analysis of CA125 in real human serum samples
(Tubitak Scientific & Technological Research Council Turkey, 2025) Afşar, Meltem; Vural, Berfin; Bilgi Kamaç, Melike; Sezgintürk, Mustafa Kemal
Several biomarkers have been developed to track the development of ovarian cancer and identify the illness at an early stage. A promising development for ovarian cancer is using cancer antigen 125 (CA125). The objective of this work is to identify the CA125 marker by utilizing an indium tin oxide polyethylene electrode. The advantage of the designed biosensor is that it is very cheap, disposable, practical, and easy to use. The necessary parameters for the developed biosensor have been optimized in detail. Repeatability, reproducibility, regeneration, storage, and selectivity studies have been completed to characterize the proposed biosensor. Electrochemical impedance spectroscopy and cyclic voltammetry techniques were used for all experimental investigations of the proposed biosensor. The immunosensor offers a large linear detection range (0.01-100 pg/mL). Moreover, this developed sensor has a 0.018 pg/mL limit of detection and a 0.06 pg/mL limit of quantification. The high accuracy of this biosensor was observed in five commercial human serums.
3D-Printable, Self-Stiffening (4D) and Shape Morphing Hydrogel through Single-Step Orthogonal Crosslinking of Phenolic Biopolymers for Dynamic Tissue Engineering
(Wiley, 2025) Güngör, Nuriye Nazet; Kurt, Tuğçe; Sarı, Buse; Işık, Melis; Okesola, Babatunde O.; Arslan, Yavuz Emre; Derkuş, Burak
Particularly for dynamic, shape-changing, or fibrillar tissues such as muscles and blood vessels, the development of innovative biomaterials is crucial for advancing tissue engineering and regenerative medicine. This study introduces a novel multicomponent hydrogel created from silk fibroin (SF), tyramine-modified hyaluronic acid (HA_Tyr), and tyramine-modified gelatin (G_Tyr). Using an enzymatic orthogonal covalent bonding between phenolic groups, i.e., tyrosine and tyramine moieties of SF, HA_Tyr, and G_Tyr, a dynamically stiffening SF/HA_Tyr/G_Tyr (SHG) multicomponent hydrogel is achieved with enhanced mechanical properties. Utilizing an extrusion-based 3D printing approach, the precise fabrication of constructs with tailored geometries and functionalities is demonstrated. The emerging 3D-printed hydrogels undergo morphologic changes (4D) under 37 °C/phosphate buffer saline (PBS) conditions. The observed morphological change results from the conformational change and folding of SF leading to fibrillation. These multicomponent hydrogels also show significant promise in creating bio-instructive materials that meet the mechanical and functional requirements necessary for in situ tissue engineering. The study highlights the potential of these self-stiffening biomaterials to recover dynamic and fibrillar tissues, supported by both in vitro and pre-clinical chorioallantoic membrane (CAM) model evaluations that underscore their biocompatibility and pro-angiogenic properties.
Transformation of Cytotoxic Linear Polyethyleneimine (L-PEI) Into Biocompatible and Hemacompatible Crosslinked Particles With Antimicrobial Properties
(Wiley, 2025) Demirci, Şahin; Sağbaş Suner, Selin; Şahiner, Mehtap; Güven, Olgun; Şahiner, Nurettin
Linear polyethyleneimine (L-PEI) microgel/particles were prepared using glycerol diglycidyl ether (GDE) as crosslinker at various mole ratios, 10%, 25%, and 50% of L-PEI repeating unit, and designated as L-PEI-1, L-PEI-2, and L-PEI-3, respectively. All microgels were found blood compatible with < 2% hemolysis and > 80% blood clotting indexes at 1000 μg/mL concentrations, whereas L-PEI is hemolytic (> 80% hemolysis). Similarly, L-PEI showed cytotoxicity on L929 fibroblasts even at 50 μg/mL, while > 80% cell viability was observed for L-PEI-based particles even at 1000 μg/mL. Although it is regarded as less cytotoxic than branched PEI, the cross-linked L-PEI particles effectively circumvent cytotoxicity as the most important limitation of L-PEI in biological applications. Additionally, the higher antimicrobial activity against Escherichia coli, gram-negative bacteria, Bacillus subtilis, gram-positive bacteria strains, and Candida albicans, yeast strains was observed for L-PEI-1 and protonated L-PEI-1 particles. Moreover, the antibacterial activity of L-PEI-1 particles was further improved after the inclusion of Ag(I) and Cu(II) ions within L-PEI particles as Ag@L-PEI-1 and Cu@L-PEI-1 composites, respectively. The lower MIC and MBC values were determined for Ag@L-PEI-1 particles against Escherichia coli and Bacillus subtilis as 0.079 and 0.312 mg/mL, respectively, and an MFC value for Candida albicans was 0.156 mg/mL for Ag@L-PEI-1 particles.
Electrochemical bioplatform for the determination of the most common and carcinogenic dibutyl phthalate migrated from daily-use plastic products
(Springer Wien, 2025) Aydın, Elif Burcu; Aydın, Muhammet; Sezgintürk, Mustafa Kemal
Dibutyl phthalate (DBP) is a typical plasticizer used in the production and processing of plastic items. Given that DBP is easily leached and discharged into the environment, posing a threat to nature and living things, a facile and feasible impedimetric magneto biosensor for ultrasensitive determination of DBP was constructed first of all by using epoxy-functionalized magnetic nanoparticles as a sensing platform. A magnetic field on the electrode surface kept the epoxy-functionalized magnetic nanoparticles in place, and the particular interaction was conducted on the solid platform of magnetic nanoparticles. This sensing platform with a large surface area enhanced to attach a large amount of DBP-specific aptamer leads to a further increase in the sensitivity of the aptasensor. The DBP-specific aptamer was covalently attached to the epoxy groups of the 3-(glycidoxypropyl)trimethoxysilane (GPMS) agent standing at the magnetic nanoparticle surface without using any crosslinking agent. Meanwhile, the DBP aptamer attached to the as-prepared epoxy-functionalized magnetic nanoparticles via amide bonds specifically recognized the DBP analyte. The interactions of DBP with the aptamer on the epoxy-functionalized magnetic nanoparticles were studied by different electrochemical techniques, and for the quantification of DBP, electrochemical impedance spectroscopy (EIS) was utilized. In the best conditions, the electrochemical aptasensor showed good results for measuring DBP, with a broad range from 1 to 200 pg/mL and a very low detection limit (LOD) of 0.32 pg/mL. Importantly, the resulting aptasensor had excellent repeatability and reproducibility, desirable specificity, long storage stability, and high sensitivity, indicating its potential applications in food and environmental safety. In addition, the real sample experiments performed on the daily-use plastic products revealed the feasibility of the proposed detection approach.
A Comparative Study of Nitrogen Doped Carbon Dots Prepared from Linear Polyethyleneimine (L-PEI) and Branched Polyethyleneimine (B-PEI): Thermal, Optical, Biocompatibility, Sensor, Antibacterial, and Light-Induced Antibacterial Activity
(Springer/Plenum Publishers, 2025) Demirci, Şahin; Sağbaş Suner, Selin; Şahiner, Mehtap; Akçalı, Alper; Güven, Olgun; Şahiner, Nurettin
The N-doped carbon dots (CDs) prepared in the presence of citric acid (CA) as carbon source, using linear poly(ethylene imine) (L-PEI) and branched poly(ethylene imine) (B-PEI) as nitrogen doping agents were prepared via hydrothermal/solvothermal technique at 250 degrees C in a Teflon-lined autoclave. The zeta potentials of L-PEI CDs and B-PEI CDs were determined as + 9.8 +/- 4 and 0.1 +/- 0.5 mV with 47 +/- 3 and 54 +/- 5 nm sizes, respectively. Bandgap values for L-PEI CDs and B-PEI CDs were estimated as 3.06 and 3.37 eV, respectively. The fluorescence intensities of L-PEI CDs and B-PEI CDs were measured as 59,310(a.u.), and 46,370(a.u.) at 474 and 442 nm, respectively, under 400 and 320 nm excitation wavelengths and 700 V PMT voltage. A higher quantum yield% value was observed for L-PEI CDs with 37 +/- 2.9%. Both L-PEI CDs and B-PEI CDs were found biocompatible up to 1000 mu g/mL concentration with > 80% L929 fibroblast cell viability. The limit of detection (LOD) values for Fe3+ by L-PEI CDs and B-PEI CDs were determined as 0.58 and 2.14 mg/mL, respectively. Both L-PEI CDs and B-PEI CDs at 10 mg/mL concentration resulted in 50% bacterial killing for gram-negative E. coli, gram-positive B. subtilis, and yeast C. albicans microorganisms. Also, the light activation of L-PEI CDs and B-PEI CDs induces almost 70 and 40% bacterial killing for gram-positive B. subtilis in 30 min. Moreover, they have a limit of detection (LOD) value of 0.58 mg/mL for Fe3+ ions, in addition to exhibiting biocompatibility and antibacterial properties.
Investigation of gas sensing properties of organic π–π* complexes spin coated thin films in dry and humid environment
(Springer, 2025) Şen, Sibel; Özbek, Zikriye; Aydın, Fatma; Capan, R.
Exposure to gaseous pollutants in both enclosed and open environments poses a significant risk to human health. In this study, an optical detection-based sensor was developed for the selective detection of toxic ammonia gas in the environment at low concentrations. Three newly synthesized organic π–π* charge-transfer (CT) complexes—anthracene picrate (ANTPc), phenanthrene picrate (PHENPc), and pyrene picrate (PYRPc)—obtained from polynuclear aromatic hydrocarbons and picric acid precursors (ANTP, PHENP, and PYRP, respectively), were evaluated for the first time as sensing thin film materials in gas sensor applications. These complexes were transferred as thin films onto the substrates using the spin-coating method for structural characterization and investigation of their sensor properties. The sensor analyses were conducted using the surface plasmon resonance (SPR) method. It was observed that the PHENPc-, ANTPc-, and PRYPc-based thin film sensors were highly selective to ammonia with the sensitivity values of 0.00007, 0.0003, and 0.0017 ppm−1, respectively. Although they are very similar to each other in terms of chemical structure, the thin film sensors produced from these three materials exhibited different responses to the vapors at room temperature. In the last section of the study, repeated gas detection measurements conducted under varying humidity conditions demonstrated that humidity affects the sensitivity of the sensors.
Single, Double, and Multiple-Heteroatoms Doped Carbon Quantum Dots as Effective Light-Induced Antimicrobial Materials
(Springer/Plenum Publishers, 2025) Sağbaş Suner, Selin; Şahiner, Mehtap; Demirci, Şahin; Şahiner, Nurettin
Single-, dual-, and multi-heteroatoms such as N, S, and B-doped carbon quantum dots (CQ-dots) were prepared to determine their dopant effects on anti-pathogenic activities. The CQ-dots were prepared using maleic acid (MA), poly(vinyl amine) (PVAm), cysteine (Cys), and boric acid (BA) as carbon, nitrogen, sulfur, and boron sources, respectively. In 345–415 nm emission wavelength range, 45.9 ± 2.4% quantum yield for dual heteroatom-doped (N/B-doped) CQ-dots were attained. Antimicrobial studies revealed that N-doped CQ-dots have significant antimicrobial susceptibility to both bacteria and fungi. The zeta potential value of N-doped CQDs had -4.9 mV was changed to -9.2 and -11.5 mV upon N/S- and N/B-doping, respectively. N/B-doped CQ-dots afforded the highest antibacterial activity providing a 1.56 mg/mL minimum inhibitory concentration (MIC) value against Escherichia coli, whereas N/S-doped CQ-dots had the highest antimicrobial activity against Staphylococcus aureus and Candida albicans yeast, 0.37 mg/mL MIC values. The photodynamic antimicrobial studies of N-, N/S-, N/B-, and N/S/B-doped CQ-dots significantly eradicated the bacteria and fungus colony upon UV-A light exposure for 30 min, with > 50% microbial colonial inhibitions. Both N and N/S-doped CQ-dots exhibit higher biofilm eradication/inhibition efficacy on Candida albicans biofilm, and all CQ-dots are biocompatible according to blood compatibility and cytotoxicity analysis at 1000 μg/mL.
Gold and silver nanoparticle decorated biocompatible and antibacterial xanthan gum/poly (HEMA-co-APTMACl) hydrogels for wound healing
(Springernature, 2025) Savranoğlu Kulabaş, Seda; Atlı, İlknur; Atalay, Hazal Nazlıcan; Boyuneğmez Tümer, Tuğba; Özay, Hava; Özay, Özgür
A delayed skin wound healing process increases the risk of infection and necessitates implementing effective treatment strategies. From this perspective, biocompatible and antibacterial hydrogels stand out as innovative biomaterials that support wound healing. In this research, a semi-interpenetrating polymer network (s-IPN) strategy was used to improve the mechanical strength, cytocompatibility, and antibacterial properties of natural polysaccharide-based hydrogels. In this context, natural polymer-based XG/poly (HEMA-co-APTMACl) hydrogels were synthesized by redox polymerization reaction. The stability of metal nanoparticles was ensured by utilizing the rich polyphenol and flavonoid components of C. Orientalis. The synthesized hydrogels were in situ functionalized with biosynthesized silver (55 nm) and gold (56 nm) nanoparticles to enhance their biocompatibility and biofunctionality. Their antibacterial activity was assessed against P. aeruginosa, S. aureus, B. cereus, and E. faecalis using the disk diffusion method. Hydrogelfilm@Ag inhibited all tested bacterial strains (7.5–8.8 mm), while Hydrogelfilm@Au exhibited stronger antibacterial activity, particularly against E. faecalis (10.3 mm) and B. cereus (9.7 mm). In contrast, the Hydrogelfilm was only effective against S. aureus (7.7 mm). The hydrogel formulations were tested for cytocompatibility and wound healing potential using HUVECs. All hydrogels composites (Hydrogelfilm@Au, @Ag, and @HF) were non-toxic and exhibited enhanced biocompatibility, promoting significant cell proliferation at all tested concentrations (5–20%). In wound healing assays, Hydrogelfilm@HF achieved complete wound closure within 12 h even at a 5% concentration, demonstrating superior regenerative potential. Overall, hydrogels incorporating green-synthesized silver and gold nanoparticles demonstrated excellent antibacterial and wound-healing properties, highlighting their promise as advanced biomaterials for tissue regeneration applications.
Enhanced antimicrobial and anticancer activities of zein protein-agarose@Au composite hydrogel for controlled release of silibinin in colon cancer therapy
(Elsevier, 2025) Atlı, İlknur; Ilgın, Pınar; Karabayır, Elif Sultan; Özay, Hava; Özay, Özgür
In this study, biodegradable hydrogel films based on natural proteins and polysaccharides were synthesized in order to improve controlled drug release, cytocompatibility and antibacterial properties. Biosynthesized gold nanoparticles were incorporated in situ cancer drug-loaded hydrogels to enhance their antibacterial, biocompatible, and cytotoxic characteristics. Then, in order to investigate the association of silibinin drug used in cancer treatment with AuNPs, drug release profile was evaluated under different environmental conditions and release kinetics were analyzed. In addition, antibacterial effects were determined by testing on Gram-negative and Gram-positive bacteria by Disk Diffusion method. As a result, cytocompatibility of silibinin drug and AuNPs in hydrogel networks and their effects on cancer cells were determined by MTT assay using human dermal fibroblasts (CCD1079KSk) and colon cancer (HT-29) cell lines. Thus, AuNPs created a synergistic effect in cancer treatment and strengthened the effect of Silibinin on tumor cells. In addition, it was determined that it exhibited a biocompatible structure by showing minimal toxicity to healthy human skin cells. This innovative drug delivery system has the potential to offer a biomaterial-based alternative in both cancer treatment and wound dressing material with its controlled release feature.
Hydrogen generation from the hydrolysis of piperazine bisborane as new hydrogen carrier material catalyzed by Ru0 nanoparticles embedded in agarose biofilms
(Pergamon-Elsevier Science Ltd, 2025) Özay, Hava; Ilgın, Pınar; Atlı, İlknur; Özay, Özgür
In this study, firstly, piperazine bisborane (PBB) was synthesized and characterized as a solid hydrogen carrier material. Subsequently, a new catalytic system, agarose hydrogel@Ru (AGH@Ru), in which nanosized Ru0 particles were homogeneously dispersed and used as a catalyst for hydrogen production from the hydrolysis of PBB, was prepared. After the structural and morphological characterization of the catalyst, for the first time in the literature, catalytic hydrogen production from the hydrolysis of PBB was initiated. As a result of catalytic hydrolysis reactions conducted under different reaction conditions, it was determined that AGH@Ru achieved 100 % efficiency in the hydrolysis reaction and produced 6 mol of hydrogen per mole of PBB. The activation parameters for the hydrolysis reaction of PBB catalyzed by AGH@Ru were calculated as Ea = 74.95 kJ mol−1, ΔH# = 72.39 kJ mol−1, and ΔS# = −77.74 J mol−1 K−1. The AGH@Ru catalytic system, with a turnover frequency (TOF) of 3.24 min−1 or 194.4 h−1 at 25 °C, also exhibited excellent reusability.
Dual use of poly(3-sulfopropyl acrylate)/pectin hydrogels functionalized with silver and quantum dots for antibacterial wound care and sustained doxorubicin delivery
(Taylor & Francis Inc, 2025) Atlı, İlknur; Özay, Özgür
In this study, poly(3-sulfopropyl acrylate)/pectin hydrogels were synthesized by redox polymerization and their usability was demonstrated for two different applications. Silver nanoparticles used for the modification of the hydrogels were obtained by in-situ green synthesis in a hydrogel matrix using avocado pit extract and their antibacterial properties were investigated. In addition, hydrogels modified with quantum dots were used for the release of doxorubicin, a cancer drug. The synthesized hydrogels and composites were characterized by TEM, SEM, XRD, FT-IR, TGA and swelling tests. The p(SPA)/pectin@Ag hydrogel modified with silver nanoparticles showed strong antibacterial activity after cefazolin loading and achieved 91.6% cumulative drug release in approximately 300 h, conforming to Weibull kinetics. The p(SPA)/pectin@QD hydrogel composites modified with cadmium sulfide quantum dots exhibited high doxorubicin loading capacity and a pH-sensitive stable release profile, reaching 81.2% cumulative drug release in approximately 300 h. This behavior, which fits the Korsmeyer-Peppas model, demonstrates the suitability of the hydrogel composites for controlled chemotherapeutic drug delivery. In addition, cytotoxicity assay was performed on MCF-7 human breast cancer cells, and the cytotoxic effects of 10 and 5 mu g/mL doses were found to be 23.0 +/- 2.6% and 42.3 +/- 2.37%.
Decellularized tumor matrices as biomimetic cancer niche: a new perspective on cancer research and therapy
(Iop Publishing Ltd, 2025) Özüdoğru, Eren; Kurt, Tuğçe; Arslan, Yavuz Emre
Cancer is among the major causes of mortality, responsible for approximately 15% of all deaths worldwide. Despite remarkable progress in modern medicine, it remains a significant global health challenge. Nevertheless, conventional therapies such as chemotherapy and radiotherapy target healthy and malignant tissues, leading to adverse side effects, including hair loss, fatigue, and nausea, which significantly reduce patients' quality of life. Even more critically, the therapeutic response varies from patient to patient, which reduces the effectiveness of treatment. Therefore, cancer tissue engineering has evolved as a novel interdisciplinary field, aiming to develop structures that mimic the tumor microenvironment to elucidate cancer development mechanisms and devise effective treatment methods. However, producing a fully synthetic biosimilar matrix by assembling all individual ECM components remains unfeasible due to the heterogeneity and complex structure of tumor tissues, as well as the necessity of highly advanced micro- and nanoengineering techniques. Consequently, decellularization techniques have recently been applied to cancer tissues to produce biomimetic tumor models. In this review, we provided a comprehensive overview of the extracellular matrix (ECM) architecture and its role in tumor progression. We also discussed the structural differences between normal and malignant tissues. We briefly reviewed decellularization techniques and analytical approaches for ECM characterization. Emphasizing the cutting-edge research, we categorized developments into three groups: decellularized tumor-derived ECM (dT-ECM), hydrogels, and bioinks. Subsequently, we critically assessed the benefits, limitations, and potential future developments of dT-ECM-based strategies. Finally, we envision that tumor tissue engineering will provide preventive treatment approaches by developing patient-specific predictive and personalized cancer models through integrating advanced biomaterials with artificial intelligence and machine learning.
Decellularization of bovine spinal cord meninges via supercritical CO2 and evaluating the extracellular matrix performance for neural tissue engineering applications
(Mary Ann Liebert, Inc, 2024) Kurt, Tuğçe; Özüdoğru, Eren; Cengiz, Uğur; Derkuş, B.; Arslan, Yavuz Emre
[No abstract available]
3D HYDROGELS FROM DETERGENT-FREE DECELLULARIZED SPINAL CORD MENINGES REINFORCED WITH HYDROPHILIC SILK FIBROIN FOR REGENERATIVE MEDICINE APPLICATIONS
(Mary Ann Liebert, Inc, 2024) Kurt, Tuğçe; Arslan, Yavuz Emre
[No abstract available]
Unveiling Bone and Dental Regeneration Potential of Quince Seed Mucilage-Nanohydroxyapatite Scaffolds in Rabbit Mandibles
(Wiley, 2025) Çetin Genç, Çiğdem; Yılmaz-Dağdeviren, Hilal Deniz; Deniz, Yeşim; Derkuş, Burak; Değirmenci, Alpin; Arslan, Yavuz Emre
Donor-side morbidity of autografting for maxillofacial region defect regeneration has directed attention to bioengineered scaffolds. Composite scaffolds that mimic the bone extracellular matrix (ECM) are the potential candidates for defect reconstruction. Herein, a plant-based regenerative hydrogel, quince seed mucilage (QSM), was enriched with the nanohydroxyapatite (nHAp) particles to construct composite scaffolds (QSM/nHAp). The emerging scaffold is able to induce cellular spheroid formation and regenerate the critical-sized bilateral mandibular defects in rabbits. The macroscopic observations, histochemical (HC) and immunohistochemical (IHC) stainings, mu-computer tomography (CT) scanning, quantitative real time-polymerase chain reaction (qRT-PCR) analyses, and scanning electron microscopy (SEM) imaging revealed that all QSM/nHAp scaffolds were swelled with host blood, filled the whole cavity, and sustained cellular infiltration without adverse reactions. The gradual biodegradation profile of the scaffolds improved bone regeneration by releasing nHAp particles from the scaffold. Strikingly, co-development of dental and bone regeneration was observed for all QSM/nHAp groups beginning after day 21. Moreover, QSM/nHAp scaffolds induced expression (> 2-fold) of bone and dental-related gene and protein expressions at the grafted area and sustained a proper platform for maxillofacial remodeling. Therefore, we strongly believe that such biocompatible plant-based constructs, compared with conventional medical devices used in maxillofacial surgery, could support and induce simultaneous bone and dental regeneration due to the intrinsic dynamics of the material.
Early and sensitive diagnosis of celiac autoimmune disease by using carboxylic acid functionalized magnetic nanoparticles-assisted biosensing platform
(Springer Wien, 2025) Aydın, Elif Burcu; Aydın, Muhammet; Sezgintürk, Mustafa Kemal
A novel impedimetric magneto-immunosensor based on iron oxide (Fe3O4) nanoparticles coated with 3-phosphonopropionic acid (3-PPA) (functionalized magnetic beads, or FMBs) was created for the highly sensitive and selective detection of anti-tissue transglutaminase antibody (anti-tTG) in human serum. This label-less immunosensor was introduced by magnetically attaching FMBs onto the working electrode surface with a neodium magnet. The FMBs were utilized as a sensing interface and had carboxylic acid groups for tTG molecules, which could selectively link the target anti-tTG antibody. The FMBs modification steps were carried out in microcentrifuge tubes and concentrated with magnetic force before electrochemical analyses. The specific immuno-interactions on the FMBs surface were characterized by using the electrochemical and microscopic techniques, and in the presence of anti-tTG antibodies, they were captured by tTG-immobilized magnetic beads, and significant increases were observed in impedimetric response. The magneto biosensor response was linearly related to the anti-tTG antibody level in a broad linear range of 0.125-15.62 U/mL and a low detection limit (LOD) of 0.04 U/mL. Additionally, this magneto sensor was stable, repeatable, reproducible, selective, and sensitive for determination of the anti-tTG. The commercial enzyme-linked immunosorbent assay (ELISA) method was employed to compare the responses of the suggested immunosensor in actual samples. The magneto biosensor results were in good agreement with the ELISA reference technique results. Consequently, the biosensor performance in the analysis of serum samples was acceptable.
Tannic acid-based bio-MOFs with antibacterial and antioxidant properties acquiring non-hemolytic and non-cytotoxic characteristics
(Elsevier, 2025) Şahiner, Nurettin; Güven, Olgun; Demirci, Şahin; Sağbaş Suner, Selin; Şahiner, Mehtap; Arı, Betül; Can, Mehmet
Tannic acid (TA) based bio-metal phenolic networks (bio-MPNs) were prepared by using Cu(II), Zn(II), Bi(III), Ce(III), La(III), and Ti(IV) metal ions. TA-based bio-MPNs exhibited wedge-shaped pores between 16.4 and 25.8 nm pore size ranges. The higher gravimetric yield% was achieved for TA-Bi(III), and TA-Ti(IV) bio-MPNs with more than 90 %, and higher surface area was observed for TA-La(IIII) bio-MPNs as 56.2 m(2)/g with 17.3 nm average pore sizes. All TA-based MPNs are non-hemolytic with less than 5 % hemolysis ratio, whereas TA-based Bio-MPNs do not affect blood clotting with > 90 % blood clotting indexes except for TA-Cu(II) Bio-MPNs at 0.1 mg/mL concentration. Moreover, TA-Bi(III) and TA-Ce(III) Bio-MPNs were found to be safer materials showing no significant toxicity on L929 fibroblast cells at 100 mu g/mL concentration, along with TA-based Bio-MPNs prepared with Cu(II), Zn(II), La(III), and Ti(IV) metal ions that could be safely used in in vivo applications at 1 mu g/mL concentration. It has been proven by 2 different antioxidant tests that the prepared TA-based Bio-MPNs show antioxidant properties even if their TA-derived antioxidant properties decrease. Furthermore, all types of TA-based Bio-MPNs show great antimicrobial activity depending on the metal ion or microorganism types and the highest antibacterial/antifungal effect was determined for TA-Cu(II), and TA-Zn(II) Bio-MPNs with the lowest MBC/MFC values against Pseudomonas aeruginosa ATCC 10145, Bacillus subtilis ATCC 6633, and Candida albicans ATCC 10231.
A novel and mass-sensitive quartz tuning fork platform for glial fibrillary acidic protein determination
(Elsevier, 2025) Özcan, Burcu; Uludağ, İnci; Ünal, Mehmet Altay; Arı, Fikret; Sezgintürk, Mustafa Kemal; Özkan, Sibel A.
Traumatic brain injury (TBI) is a major cause of mortality and disability worldwide, and its diagnosis is still difficult. A unique blood biomarker for numerous neurological disorders, including traumatic brain injury, is glial fibrillary acidic protein (GFAP). Portable, quick, and functional equipments are crucial in fighting against neurological illnesses. Quartz Tuning Fork (QTF) biosensors are attracting great attention due to their seamless integration with miniaturized and portable devices. This study introduces a new, adaptable, affordable frequency analyzer for GFAP detection. A simple and stable process to design a QTF system which has gold surface modified with 3-mercaptopropionic acid (3-MPA) and its biocompatibility with GFAP antigen was investigated. The surface morphology of the QTF system was analyzed during its immobilization procedure using Atomic Force Microscopy (AFM). The constructed QTF sensor system demonstrated high repeatability (the standard deviation: +/- 0.12183 Hz), good reproducibility, linearity, ability to detect antigen concentrations at femtogram levels. It proved that the QTF sensor system decorated with 3-MPA could be a good choice for the sensitive determination of GFAP, according to the statistical data. The modified QTF sensor demonstrated remarkably straightforward and mass-sensitive GFAP detection. The developed QTF-based biosensor achieved an outstanding linear detection range of 1-100 fg mL-1. The responses of the designed QTF-based sensor to commercial human serum also show that it is a successful and promising system for clinical use.
MIP-based sensing strategies for the diagnosis of prostate and lung cancers
(Elsevier, 2025) Uludağ Anıl, İnci; Sezgintürk, Mustafa Kemal
Prostate and lung cancer are among the most prevalent and lethal forms of the disease, and, globally, cancer is a primary cause of mortality. Early diagnosis and the application of precise biomarkers are crucial for prolonging patient lifespan and enhancing quality of life. Molecularly imprinted polymers (MIPs) are synthetic substances capable of selectively identifying and binding specific target molecules. Besides their sensitivity to biomolecules, MIPs offer significant advantages in cancer biomarker detection due to their cost-effectiveness and flexible architectures. MIP-based biosensors designed for the detection of prostate and lung cancer-specific biomarkers provide unique solutions that enhance the diagnostic process and improve its accuracy. This study aims to assess the present state of MIP-based biosensors in the detection of biomarkers for prostate and lung cancer, as well as the potential opportunities provided by this technology. This study will present an overview of potential biomarkers for lung and prostate cancer, clarify the fundamental features of MIPs, and discuss their uses in MIPbased biosensors along with prospective future applications. In this context, our review is aimed to contribute to the development of innovative methods used in early cancer diagnosis.
Celiac disease biomarker quantification in human fluid microenvironment: A selective and ultrasensitive magnetosensing immunoplatform
(Elsevier Science Sa, 2025) Aydın, Elif Burcu; Aydın, Muhammet; Sezgintürk, Mustafa Kemal
An innovative magnetosensing strategy for highly sensitive impedimetric determination of anti-tissue transglutaminase antibodies (anti-tTG) was developed and applied successfully in real serum and saliva samples. The transducer surface of the proposed immunosensor consisted of an indium tin electrode (ITO) attached with poly (3-thienylacetic acid) (PTAc)-coated magnetic nanoparticles, which provided a very useful surface for the attachment of the biological molecules. The PTAc-coated magnetic nanoparticles were held by a magnetic field on the electrode surface, and the immunological reaction was carried out on magnetic nanoparticles as a solid platform on which the tissue transglutaminase (tTG) was covalently bound. With the specific capture of anti-tTG on the tTG-immobilized surface, an impedimetric signal was measured, and the electrochemical response of this specific reaction was correlated with the anti-tTG concentration. A linear relationship between the impedimetric signal and the anti-tTG concentration was obtained over a wide range of 0.125-25 U/mL. This magnetobiosensor illustrated a stable quantitative signal to anti-tTG concentrations after 45 minutes of incubation with a limit of detection of 0.034 U/mL and a low relative standard deviation (RSD) of 3.61 %, n = 3. This immunosensor's electrochemical behaviour was thoroughly examined, with consideration given to factors including sensitivity, specificity, repeatability, reproducibility, and storage stability. Lastly, serum and saliva samples were analyzed using the biosensor, and excellent correlation was achieved between the commercial ELISA kit and the proposed immunosensor. As a result, this approach held out a lot of hope for a straightforward, affordable, and user-friendly analytical technique that would enable the label-free measurement of anti-tTG levels.

Güncel Gönderiler