Comparative Analysis of Alfalfa Mutants in Response to Drought Stress Applied After First Cutting

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dc.authorid/0000-0001-7564-997X
dc.authoridTiryaki, iskender/0000-0002-7504-2892
dc.authoridACAR, OKAN/0000-0002-9818-8827
dc.contributor.authorCetin, Selcuk
dc.contributor.authorTiryaki, Iskender
dc.contributor.authorSari, Ugur
dc.contributor.authorAcar, Okan
dc.date.accessioned2025-01-27T20:53:50Z
dc.date.available2025-01-27T20:53:50Z
dc.date.issued2024
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThe purpose of this study was to determine the agro-morphological, physiological, and transcriptional characteristics of ethyl methane sulfonate (EMS)-mutagenized M3 alfalfa (Medicago sativa L.) mutants grown for 24 days under water deficit conditions following the first cutting at the blooming bud stage. Drought stress generally reduced all agro-morphological parameters measured. Cutting stress significantly reduced total chlorophyll contents of all genotypes, with the exception of mutant X20. Significant changes occurred in the levels of ascorbate peroxidase (APX), lipid peroxidation (TBARS), glutathione reductase (GR), superoxide dismutase (SOD) and crude protein levels. Drought stress considerably elevated GR levels in irrigated control (C1) and unirrigated control (C2) plants. In contrast to the other genotypes studied, mutant X20's crude protein level increased significantly after the first cutting under 24 days of water deficit conditions, compared to the level found before cutting. The expression profiles of Medicago truncatula Response to Desiccation 2 (MtRD2) and Medicago sativa proline dehydrogenase (MsProDH) genes differed based on the plant genotype and duration of drought stress. Drought stress increased MsProDH gene expression in X20, X50, and C2 plants by 4.57, 14.8, and 20.65 times, respectively. The findings of the expression study indicated that the MtRD2 gene may play a key role in stress reduction rather than drought stress per se. The results of this study revealed that cutting and drought are two independent stress variables that should be evaluated separately when evaluating genotype response to both irrigated and unirrigated growing environments in alfalfa.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [TOVAG-116O417]
dc.description.sponsorshipThe Scientific and Technological Research Council of Turkey (TUBITAK) funded this work through research grant TOVAG-116O417. We would like to thank Dr. Melih Taskin for allowing us to use RT-qPCR. We'd like to thank Huseyin Keles and Semun Tayyar for their assistance with harvesting and selfing in the field, respectively. We thank Dr. Martha Rowe for editing the manuscript. We thank Enes Gokhan Yilmaz, Beste Celep, Erman Cavusoglu, and anonymous lab members who assisted with the laboratory tests and mutant growth in control circumstances.
dc.identifier.doi10.1007/s10343-024-01028-1
dc.identifier.endpage1219
dc.identifier.issn2948-264X
dc.identifier.issn2948-2658
dc.identifier.issue5
dc.identifier.scopus2-s2.0-85203385663
dc.identifier.scopusqualityN/A
dc.identifier.startpage1209
dc.identifier.urihttps://doi.org/10.1007/s10343-024-01028-1
dc.identifier.urihttps://hdl.handle.net/20.500.12428/25859
dc.identifier.volume76
dc.identifier.wosWOS:001309297100004
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofJournal of Crop Health
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectMutant alfalfa
dc.subjectDrought stress
dc.subjectCutting stress
dc.subjectAgro-morphological and physiological parameters
dc.subjectTranscriptional expression
dc.titleComparative Analysis of Alfalfa Mutants in Response to Drought Stress Applied After First Cutting
dc.typeArticle

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