Decellularized spinal cord meninges extracellular matrix hydrogel that supports neurogenic differentiation and vascular structure formation

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dc.authorid0000-0002-6442-7842en_US
dc.authorid0000-0003-3445-1814en_US
dc.authorscopusid57218671880en_US
dc.authorscopusid36088908700en_US
dc.authorwosid-en_US
dc.authorwosidM-2907-2016en_US
dc.contributor.authorÖzüdoğru, Eren
dc.contributor.authorIşık, Melis
dc.contributor.authorEylem, Cemil Can
dc.contributor.authorNemutlu, Emirhan
dc.contributor.authorArslan, Yavuz Emre
dc.contributor.authorDerkus, Burak
dc.date.accessioned2024-12-11T12:10:25Z
dc.date.available2024-12-11T12:10:25Z
dc.date.issued2021en_US
dc.departmentFakülteler, Mühendislik Fakültesi, Biyomühendislik Bölümü
dc.description.abstractDecellularization of extracellular matrices offers an alternative source of regenerative biomaterials that preserve biochemical structure and matrix components of native tissues. In this study, decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges. The success of decellularization is characterized by measuring the contents of residual DNA, glycosaminoglycans, and hydroxyproline, while a proteomics analysis is applied to reveal the composition of MeninGEL. Frequency and temperature sweep rheometry show that dSCM forms self-supporting hydrogel at physiological temperature. The MeninGEL possesses excellent cytocompatibility. Moreover, it is evidenced with immuno/histochemistry and gene expression studies that the hydrogel induces growth-factor free differentiation of human mesenchymal stem cells into neural-lineage cells. Furthermore, MeninGEL instructs human umbilical vein endothelial cells to form vascular branching. With its innate bioactivity and low batch-to-batch variation property, the MeninGEL has the potential to be an off-the-shelf product in nerve tissue regeneration and restoration.en_US
dc.identifier.citationÖzüdoğru, E., Işık, M., Eylem, C. C., Nemutlu, E., Arslan, Y. E., & Derkus, B. (2021). Decellularized spinal cord meninges extracellular matrix hydrogel that supports neurogenic differentiation and vascular structure formation. Journal of Tissue Engineering and Regenerative Medicine, 15(11), 948–963. https://doi.org/10.1002/term.3240en_US
dc.identifier.doi10.1002/term.3240
dc.identifier.endpage963en_US
dc.identifier.issn1932-6254
dc.identifier.issn1932-7005
dc.identifier.issue11en_US
dc.identifier.pmid34463042
dc.identifier.scopus2-s2.0-85114355862
dc.identifier.startpage948en_US
dc.identifier.urihttps://doi.org/10.1002/term.3240
dc.identifier.urihttps://hdl.handle.net/20.500.12428/6732
dc.identifier.volume15en_US
dc.identifier.wosWOS:000693950400001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorÖzüdoğru, Eren
dc.institutionauthorArslan, Yavuz Emre
dc.language.isoen
dc.publisherJohn Wiley and Sons Ltden_US
dc.relation.ispartofJournal of Tissue Engineering and Regenerative Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAngiogenesisen_US
dc.subjectDecellularizationen_US
dc.subjectExtracellular matrixen_US
dc.subjectHydrogelen_US
dc.subjectNerve tissue engineeringen_US
dc.subjectSpinal cord meningesen_US
dc.titleDecellularized spinal cord meninges extracellular matrix hydrogel that supports neurogenic differentiation and vascular structure formation
dc.typeArticle

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