Decellularized spinal cord meninges extracellular matrix hydrogel that supports neurogenic differentiation and vascular structure formation
Yükleniyor...
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
John Wiley and Sons Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Decellularization of extracellular matrices offers an alternative source of regenerative biomaterials that preserve biochemical structure and matrix components of native tissues. In this study, decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges. The success of decellularization is characterized by measuring the contents of residual DNA, glycosaminoglycans, and hydroxyproline, while a proteomics analysis is applied to reveal the composition of MeninGEL. Frequency and temperature sweep rheometry show that dSCM forms self-supporting hydrogel at physiological temperature. The MeninGEL possesses excellent cytocompatibility. Moreover, it is evidenced with immuno/histochemistry and gene expression studies that the hydrogel induces growth-factor free differentiation of human mesenchymal stem cells into neural-lineage cells. Furthermore, MeninGEL instructs human umbilical vein endothelial cells to form vascular branching. With its innate bioactivity and low batch-to-batch variation property, the MeninGEL has the potential to be an off-the-shelf product in nerve tissue regeneration and restoration.
Açıklama
Anahtar Kelimeler
Angiogenesis, Decellularization, Extracellular matrix, Hydrogel, Nerve tissue engineering, Spinal cord meninges
Kaynak
Journal of Tissue Engineering and Regenerative Medicine
WoS Q Değeri
Q2
Scopus Q Değeri
Cilt
15
Sayı
11
Künye
Özüdoğru, E., Işık, M., Eylem, C. C., Nemutlu, E., Arslan, Y. E., & Derkus, B. (2021). Decellularized spinal cord meninges extracellular matrix hydrogel that supports neurogenic differentiation and vascular structure formation. Journal of Tissue Engineering and Regenerative Medicine, 15(11), 948–963. https://doi.org/10.1002/term.3240
