IN VITRO EVALUATION OF THE ANTIOXIDANT, ANTIBACTERIAL, ANTIBIOFILM, AND CYTOTOXIC ACTIVITY OF MENTHA PIPERITA ESSENTIAL OIL
Yükleniyor...
Tarih
2023
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Slovak University of Agriculture
Erişim Hakkı
info:eu-repo/semantics/openAccess
Attribution 3.0 United States
Attribution 3.0 United States
Özet
Nowadays, antibacterial resistance to synthetic antibiotics has become a big issue worldwide. Mentha piperita essential oil (EO) and its bioactive components with antibacterial, antibiofilm, and antioxidant properties are recommended for the treatment of microbial infections in traditional medicine. This study aimed to detect phytochemical components, antioxidant and cytotoxic activity; to evaluate antibacterial and antibiofilm effects of M. piperita EO on strains. Gas chromatography/mass spectrometry (GC-MS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) tests were used to determine chemical composition, antioxidant and cytotoxic potentials of EO, respectively. Antibacterial activity was carried out through disc diffusion and minimum inhibitory concentration (MIC) assays. The biofilm biomass of bacteria was determined by the crystal violet (CV) staining method. Menthone (27.28%), L-menthol (23.71%), and limonene (7.48%) were the main components. M. piperita EO was found to have strong antibacterial activity with the MIC-MBC values in the range of 0.125-16.00% (v/v) and moderate antioxidant activity. The peppermint EO showed an antibiofilm effect on all strains and a cytotoxic effect on NIH 3T3 cells. M. piperita EO may be used alone or in combination with antibiotics or carrier oils as a therapeutic agent against bacterial infections. Further in vivo experiments should be carried out to detect a safe dosage of EO.
Açıklama
Anahtar Kelimeler
Antibacterial, Antibiofilm, Antioxidant, Cytotoxicity, Mentha piperita
Kaynak
Journal of Microbiology, Biotechnology and Food Sciences
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
12
Sayı
5
Künye
Altun, M. (2023). IN VITRO EVALUATION OF THE ANTIOXIDANT, ANTIBACTERIAL, ANTIBIOFILM, AND CYTOTOXIC ACTIVITY OF MENTHA PIPERITA ESSENTIAL OIL. Journal of Microbiology, Biotechnology and Food Sciences, 12(5), e6206–e6206. https://doi.org/10.55251/jmbfs.6206
