Micropropagation and Acclimatization of Hypericum aucheri

dc.authorid0000-0001-7301-9288
dc.contributor.authorCambaz, Ebru
dc.contributor.authorCorduk, Nursen
dc.date.accessioned2026-02-03T12:00:00Z
dc.date.available2026-02-03T12:00:00Z
dc.date.issued2025
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractHypericum aucheri is a Hypericum species distributed in T & uuml;rkiye as well as Greece and Bulgaria with notable pharmacological potential. Its medicinal importance underlines the need for effective propagation strategies. This study developed an in vitro micropropagation protocol using nodal explants cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of cytokinins-6-benzylaminopurine (BAP) (0, 1, 2 mg L-1) or kinetin (KIN) (0, 1, 2 mg L-1)-combined with 2,4-dichlorophenoxyacetic acid (2,4-D) (0, 0.5, 1 mg L-1) for shoot induction. The highest shoot induction and multiplication (100% and multiplication coefficient of 10.0) were achieved with KIN (2 mg L-1). Rooting was most successful on MS medium supplemented with alpha-naphthaleneacetic acid (NAA) (1 mg L-1) and activated charcoal (AC) (1 mg L-1), resulting in 100% rooting. Acclimatized plantlets showed an 80% survival rate under ex vitro conditions. This study presents the first efficient micropropagation system for H. aucheri, contributing to its ex situ conservation and providing a foundation for future pharmacological and biotechnological research.
dc.identifier.doi10.3390/horticulturae11091069
dc.identifier.issn2311-7524
dc.identifier.issue9
dc.identifier.scopus2-s2.0-105017129269
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.3390/horticulturae11091069
dc.identifier.urihttps://hdl.handle.net/20.500.12428/34465
dc.identifier.volume11
dc.identifier.wosWOS:001580000100001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherMdpi
dc.relation.ispartofHorticulturae
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20260130
dc.subjectex vitro
dc.subjectHypericaceae
dc.subjectmultiplication
dc.subjectnodal explant
dc.subjectrooting
dc.subjectshoot induction
dc.titleMicropropagation and Acclimatization of Hypericum aucheri
dc.typeArticle

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