Immunosuppressive influence of parasitoid wasp Pimpla turionellae calyx fluid on host Galleria mellonella cell-mediated immune response and hemocyte viability
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Tarih
2022
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Cambridge University Press
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Endoparasitoid species devoid of symbiotic viruses inject secretions derived from their reproductive glands into their hosts during parasitism in order to avoid various immune responses of their hosts. Pimpla turionellae L. (Hymenoptera: Ichneumonidae) is an endoparasitoid that lacks polydnaviruses, and its venom has previously been shown to paralyze the host Galleria mellonella (Lepidoptera: Pyralidae) and suppress its immune reactions to ensure the egg survival. The present study demonstrates that another female-injected factor calyx fluid extracted from the P. turionellae ovary is also responsible for the suppression of G. mellonella immunity. The total hemocyte counts of G. mellonella decrease after treatment with calyx fluid in a concentration-dependent manner. Significant reductions in cell viability are also observed at all calyx fluid doses both in vivo and in vitro. The analyses of the beads injected into the insects as encapsulation targets revealed that the number of encapsulated beads reduced significantly compared to controls post-calyx fluid injection. The injection of the highest calyx fluid dose (1 female equivalent calyx) is sufficient to completely inhibit the strong encapsulation and melanization reactions of the last instar larvae 24 h post-injection. These results demonstrate that P. turionellae calyx fluid is required to regulate host immunity for successful parasitization.
Açıklama
Anahtar Kelimeler
Apoptosis, Calyx, Encapsulation, Galleria mellonella, Hemocyte, Pimpla turionellae
Kaynak
Bulletin of Entomological Research
WoS Q Değeri
Q2
Scopus Q Değeri
Cilt
112
Sayı
3
Künye
Kaya, S., Uçkan, F., & Er, A. (2022). Immunosuppressive influence of parasitoid wasp pimpla turionellae calyx fluid on host galleria mellonella cell-mediated immune response and hemocyte viability. Bulletin of Entomological Research, 112(3), 361-369. doi:10.1017/S0007485321000924
