Assessment of genotoxic and cytotoxic effects in COVID-19 patients

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dc.contributor.authorAliravci, Isil Deniz
dc.contributor.authorBerber, Ahmet Ali
dc.contributor.authorKenanoglu, Nihan Akinci
dc.contributor.authorErtekin, Yusuf Haydar
dc.date.accessioned2025-05-29T02:57:30Z
dc.date.available2025-05-29T02:57:30Z
dc.date.issued2025
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractPurpose: The objective of our study was to ascertain whether the SARS-CoV-2 virus exerts direct cytotoxic and genotoxic effects on human blood defence cells. Materials and Methods: An in vitro analysis was conducted to assess the cytotoxic and genotoxic effects of the virus using three established tests: the mitotic index (MI), micronucleus (MN), and comet assay (CA). These tests were applied to blood samples from 101 patients. The blood samples were simultaneously analyzed using the polymerase chain reaction (PCR) test. The study population included patients of all ages and genders who presented to the outpatient clinic with symptoms suggestive of a respiratory tract infection and fever. Results: The frequency of MN in the human lymphocytes of COVID-19-infected patients (1.06) was higher compared to COVID-19-negative patients (0.68). Similarly, in COVID-19-positive individuals, parameters such as tail length (3.67), tail moment (1.786), and tail intensity in the comet assay showed a significant increase compared to the negative control, indicating DNA damage. In the cytotoxicity assessment, the MI frequency of COVID-19-positive individuals (0.041) was significantly lower than that of negative controls (0.051). Gender did not influence the cyto/genotoxicity (except for tail length) in SARS-CoV-2-infected patients. Among age groups, the SARS-CoV-2 virus increased MI frequency and tail intensity only in middle-aged individuals (26-36 years). Conclusion: The SARS-CoV-2 virus has the potential to induce cytotoxic and genotoxic effects in the human lymphocytes of infected individuals.
dc.description.sponsorshipanakkale Onsekiz Mart University Scientific Research Projects Coordination Unit [TSA-2022-4076]
dc.description.sponsorshipThis work was supported by Canakkale Onsekiz Mart University Scientific Research Projects Coordination Unit, Project number: TSA-2022-4076.
dc.identifier.doi10.17826/cumj.155717
dc.identifier.endpage21
dc.identifier.issn2602-3032
dc.identifier.issn2602-3040
dc.identifier.issue1
dc.identifier.scopusqualityN/A
dc.identifier.startpage11
dc.identifier.urihttps://doi.org/10.17826/cumj.155717
dc.identifier.urihttps://hdl.handle.net/20.500.12428/30085
dc.identifier.volume50
dc.identifier.wosWOS:001456063300002
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherCukurova Univ, Fac Medicine
dc.relation.ispartofCukurova Medical Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250529
dc.subjectComet assay
dc.subjectCOVID-19
dc.subjectgenotoxicity
dc.subjectmicronucleus
dc.subjectmitotic index
dc.titleAssessment of genotoxic and cytotoxic effects in COVID-19 patients
dc.typeArticle

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