Identification and expression of strigolactone biosynthesis and signaling genes and the in vitro effects of strigolactones in olive (Olea europaea L.)

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dc.authoridSezer, Fatih / 0000-0002-9436-0191
dc.authoridTaşkın, Kemal Melih / 0000-0002-3746-0508
dc.authoridÖzbilen, Aslıhan / 0000-0002-2034-4682
dc.contributor.authorÖzbilen, Aslıhan
dc.contributor.authorSezer, Fatih
dc.contributor.authorTaşkın, Kemal Melih
dc.date.accessioned2025-01-27T20:41:28Z
dc.date.available2025-01-27T20:41:28Z
dc.date.issued2024
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractStrigolactones (SLs), synthesized in plant roots, play a dual role in modulating plant growth and development, and in inducing the germination of parasitic plant seeds and arbuscular mycorrhizal fungi in the rhizosphere. As phytohormones, SLs are crucial in regulating branching and shaping plant architecture. Despite the significant impact of branching strategies on the yield performance of fruit crops, limited research has been conducted on SLs in these crops. In our study, we identified the transcript sequences of SL biosynthesis and signaling genes in olive (Olea europaea L.) using rapid amplification of cDNA ends. We predicted the corresponding protein sequences, analyzed their characteristics, and conducted molecular docking with bioinformatics tools. Furthermore, we quantified the expression levels of these genes in various tissues using quantitative real-time PCR. Our findings demonstrate the predominant expression of SL biosynthesis and signaling genes (OeD27, OeMAX3, OeMAX4, OeMAX1, OeD14, and OeMAX2) in roots and lateral buds, highlighting their importance in branching. Treatment with rac-GR24, an SL analog, enhanced the germination frequency of olive seeds in vitro compared with untreated embryos. Conversely, inhibition of SL biosynthesis with TIS108 increased lateral bud formation in a hard-to-root cultivar, underscoring the role of SLs as phytohormones in olives. These results suggest that modifying SL biosynthesis and signaling pathways could offer novel approaches for olive breeding, with potential applicability to other fruit crops.
dc.description.sponsorshipCOST Action [FA1206]; Scientific and Technological Research Council of Turkey(TUBITAK) [215O543]; [FA12063]
dc.description.sponsorshipCOST Action, Grant/Award Numbers: FA12063, FA1206; Scientific and Technological Research Council of Turkey(TUBITAK), Grant/Award Number: 215O543
dc.identifier.doi10.1002/pld3.568
dc.identifier.issn2475-4455
dc.identifier.issue2
dc.identifier.pmid38405354
dc.identifier.scopus2-s2.0-85185932784
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1002/pld3.568
dc.identifier.urihttps://hdl.handle.net/20.500.12428/24154
dc.identifier.volume8
dc.identifier.wosWOS:001175267400001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherJohn Wiley & Sons Ltd
dc.relation.ispartofPlant Direct
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectGR24
dc.subjectolive
dc.subjectqPCR
dc.subjectstrigolactones
dc.subjectTIS108
dc.subjecttissue culture
dc.titleIdentification and expression of strigolactone biosynthesis and signaling genes and the in vitro effects of strigolactones in olive (Olea europaea L.)
dc.typeArticle

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