Evaluation of the Effects of Some Raphanus sativus Tuber Extracts on Certain Antioxidant Enzyme Activity and Lipid Peroxidation Level Against Gray Mold (Botrytis Cinerea) in Vicia Faba Leaves

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dc.authoridKaya, Nergis / 0000-0002-4206-1149
dc.authoridKaya, Tayfun / 0000-0002-6808-3338
dc.authoridYiğit, Soner / 0000-0002-2899-2246
dc.contributor.authorKaya, Nergis
dc.contributor.authorKaya, Tayfun
dc.contributor.authorYiğit, Soner
dc.date.accessioned2025-01-27T19:20:26Z
dc.date.available2025-01-27T19:20:26Z
dc.date.issued2023
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThe antioxidant defense system of the medicinal plant Raphanus sativus L. tuber root in terms of certain antioxidant enzymes (ascorbat peroxidase, glutathion reductase, catalase, superoxide dismutase) and lipid peroxidation against gray mold disease caused by Botrytis cinerea fungus on Vicia faba L. (bean) leaves was analyzed spectrophotometrically. V. faba plants were grown from seed under controlled conditions. A spore suspension of 105 spores/ml of B. cinerea was prepared. The leaves of V. faba were treated with 1-) only distilled water, 2-)only DMSO, 3-) only B. cinerea (positive control), 4-) only extract applications (distilled water, ethanol and methanol extract),5-) B. cinerea treatment after extract applications (extract:fungus). The leaves of V. faba were harvested 24 and 48 hours after the applications. Analyzes of the supernatants obtained from the extracts were performed spectrophotometrically. It has been revealed that the activity of all investigated enzymes generally increased in the extract:fungus application groups compared to the fungus application group. It was determined that the application of fungus alone caused an increase in the activity of enzymes compared to application groups other than the extract:fungus application groups. It was observed that there was an increase in lipid peroxidation (MDA) only in the fungus application group compared to all extract:fungus application groups except 10mg/ml distilled water extract:fungus treatment. As a result of the research, it was determined that the extract:fungus applications generally increased the antioxidant enzyme activity levels and decreased the MDA content when compared to the negative control and positive control groups. In extract:fungus applications, enzyme activities were obtained from 10mg/ml distilled water extract:fungus, 10mg/ml EtOH extract:fungus, 10mg/ml MeOH extract:fungus applications. From this point of view, it can be stated that R. sativus distilled water, ethanol and methanol extracts generally give an increased antioxidant defense response in V. faba leaves compared to B. cinerea inoculation alone.
dc.identifier.doi10.16984/saufenbilder.1132730
dc.identifier.endpage38
dc.identifier.issn1301-4048
dc.identifier.issn2147-835X
dc.identifier.issue1
dc.identifier.scopus2-s2.0-85218162257
dc.identifier.scopusqualityQ4
dc.identifier.startpage22
dc.identifier.trdizinid1158736
dc.identifier.urihttps://doi.org/10.16984/saufenbilder.1132730
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1158736
dc.identifier.urihttps://hdl.handle.net/20.500.12428/14865
dc.identifier.volume27
dc.indekslendigikaynakTR-Dizin
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSakarya University
dc.relation.ispartofSakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_TRD_20250125
dc.subjectVicia faba L.
dc.subjectRaphanus sativus L.
dc.subjectBotrytis cinerea
dc.subjectantioxidant enzymes
dc.subjectlipid peroxidation
dc.titleEvaluation of the Effects of Some Raphanus sativus Tuber Extracts on Certain Antioxidant Enzyme Activity and Lipid Peroxidation Level Against Gray Mold (Botrytis Cinerea) in Vicia Faba Leaves
dc.typeArticle

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