Development of plant-based biopolymer coatings for 3D cell culture: boron-silica-enriched quince seed mucilage nanocomposites

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dc.authorid0000-0002-0400-3351en_US
dc.authorid0000-0003-3445-1814en_US
dc.authorscopusid37064299600en_US
dc.authorscopusid36088908700en_US
dc.authorwosidF-6410-2011en_US
dc.authorwosidM-2907-2016en_US
dc.contributor.authorYılmaz, Hilal Deniz
dc.contributor.authorCengiz, Uğur
dc.contributor.authorDerkuş, Burak
dc.contributor.authorArslan, Yavuz Emre
dc.date.accessioned2024-01-24T13:23:00Z
dc.date.available2024-01-24T13:23:00Z
dc.date.issued2023en_US
dc.departmentFakülteler, Mühendislik Fakültesi, Biyomühendislik Bölümü
dc.departmentFakülteler, Mühendislik Fakültesi, Kimya Mühendisliği Bölümü
dc.description.abstractSpheroid formation with spontaneous aggregation has captured interest in most cell culture studies due to its easy set-up and more reliable results. However, the economic and technical costs of the advanced systems and commercial ultra-low adhesive platforms have pushed researchers into pursuing alternatives. Nowadays, polymeric coatings, including poly-hydroxyethyl methacrylate and agar/agarose, are the commonly used polymers for non-adhesive plate fabrication, yet the costs and working solvent or heat-dependent preparation procedures maintain the need for the development of novel biomaterials. Here, we propose a greener and more economical approach for producing non-adherent surfaces and spheroid formation. For this, a plant waste-based biopolymer from quince fruit (Cydonia oblonga Miller, from Rosaceae family) seeds and boron-silica precursors were introduced. The unique water-holding capacity of quince seed mucilage (Q) was enriched with silanol and borate groups to form bioactive and hydrophilic nanocomposite overlays for spheroid studies. Moreover, 3D gel plates from the nanocomposite material were fabricated and tested in vitro as a proof-of-concept. The surface properties of coatings and the biochemical and mechanical properties of the nanocomposite materials were evaluated in-depth with techniques, and extra hydrophilic coatings were obtained. Three different cell lines were cultured on these nanocomposite surfaces, and spheroid formation with increased cellular viability was recorded on day 3 with a >200 μm spheroid size. Overall, Q-based nanocomposites are believed to be a fantastic alternative for non-adherent surface fabrication due to their low-cost, easy operation, and intrinsic hydration layer forming capacity with biocompatible nature in vitro.en_US
dc.identifier.citationYılmaz, H. D., Cengiz, U., Derkuş, B., & Arslan, Y. E. (2023). Development of plant-based biopolymer coatings for 3D cell culture: boron–silica-enriched quince seed mucilage nanocomposites. Biomaterials Science, 11(15), 5320–5336. https://doi.org/10.1039/D3BM00170Aen_US
dc.identifier.doi10.1039/D3BM00170A
dc.identifier.endpage5336en_US
dc.identifier.issn2047-4830
dc.identifier.issn2047-4849
dc.identifier.issue15en_US
dc.identifier.pmid37358798
dc.identifier.scopus2-s2.0-85164338952
dc.identifier.startpage5320en_US
dc.identifier.urihttps://doi.org/10.1039/D3BM00170A
dc.identifier.urihttps://hdl.handle.net/20.500.12428/5391
dc.identifier.volume11en_US
dc.identifier.wosWOS:001016233900001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorCengiz, Uğur
dc.institutionauthorArslan, Yavuz Emre
dc.language.isoen
dc.publisherRoyal Society of Chemistryen_US
dc.relation.ispartofBiomaterials Scienceen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject3D cell cultureen_US
dc.subjectPlant-based biopolymer coatingsen_US
dc.subjectCell Culture Techniquesen_US
dc.subjectSilicon Dioxideen_US
dc.subjectSeedsen_US
dc.titleDevelopment of plant-based biopolymer coatings for 3D cell culture: boron-silica-enriched quince seed mucilage nanocomposites
dc.typeArticle

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