A disposable and ultrasensitive immunosensor for the detection of HE4 in human serum samples

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dc.authoridBilgi Kamac, Melike/0000-0002-3381-7522
dc.contributor.authorVural, Berfin
dc.contributor.authorCaliskan, Meltem
dc.contributor.authorBilgi Kamac, Melike
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2025-01-27T20:49:38Z
dc.date.available2025-01-27T20:49:38Z
dc.date.issued2024
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractOvarian cancer is described as excessive cell proliferation in the ovaries. It is thought that ovarian cancer and the human epididymis protein 4 (HE4) are closely linked. HE4 is approved as a critical biomarker for the diagnosis and the progression monitoring of the ovarian cancer. In this work, an impedimetric biosensor was fabricated for the analysis of HE4 by modifying the surface of disposable ITO-PET (indium tin oxide-polyethylene terephthalate) sheet with a certain dimensions by 3-Aminopropyl trimethoxy silane (3-APTES). For the interaction between the amino ends of 3-APTES and anti-HE4 antibodies glutaraldehyde was used as a cross-linker. Electrochemical impedance (EIS) and cyclic voltammetry (CV) methods were applied in all electrochemical measurements. Important optimization and characterization experiments have also been completed. Under the optimum conditions, the concentration range for HE4 was obtained between 1 pg/mL and 3000 pg/mL with an LOD and LOQ values of 0.094 pg/mL and 0.3134 pg/mL, respectively. Selectivity experiments revealed that the biosensor showed a great selectivity with its target HE4. The biosensor also showed a good reproducibility with a relative standard deviation (RSD) of slope data calculated as 2.443%. Finally, we applied the biosensor to the commercial real human serum samples for analysis of HE4 and the results of these experiments were encouraging to be used in clinical settings.
dc.description.sponsorshipTrkiye Bilimsel ve Teknolojik Arascedil;timath;rma Kurumu
dc.description.sponsorshipNo Statement Available
dc.identifier.doi10.1007/s11696-024-03359-9
dc.identifier.endpage3342
dc.identifier.issn0366-6352
dc.identifier.issn2585-7290
dc.identifier.issue5
dc.identifier.scopus2-s2.0-85185925209
dc.identifier.scopusqualityQ2
dc.identifier.startpage3331
dc.identifier.urihttps://doi.org/10.1007/s11696-024-03359-9
dc.identifier.urihttps://hdl.handle.net/20.500.12428/25253
dc.identifier.volume78
dc.identifier.wosWOS:001171582700001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer Int Publ Ag
dc.relation.ispartofChemical Papers
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectOvarian cancer
dc.subjectDisposable biosensor
dc.subjectFlexible working electrodes
dc.subjectHuman epididymis protein 4
dc.subjectHE4
dc.subject3-APTES
dc.titleA disposable and ultrasensitive immunosensor for the detection of HE4 in human serum samples
dc.typeArticle

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