Colloidal bioactive nanospheres prepared from natural biomolecules, catechin and L-lysine

Basit Öğe Kaydı
dc.authoridCAN, Mehmet/0000-0002-5993-206X
dc.contributor.authorCan, Mehmet
dc.contributor.authorŞahiner, Mehtap
dc.contributor.authorŞahiner, Nurettin
dc.date.accessioned2025-01-27T20:34:40Z
dc.date.available2025-01-27T20:34:40Z
dc.date.issued2022
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractRecently, major attention has been devoted to exploring and increase biomedical applications of catechins by directly employing them as constituents of nano-vehicles. Here, (+)-catechin (CAT) was integrated with another benign biomolecule, L-Lysine (LYS) using formaldehyde (FA) via single-step Mannich condensation reaction and self-assembled supramolecular CAT-LYS networks were synthesized. By using various molar feed ratios of CAT, LYS, and FA (CAT:LYS:FA), different formulations of CAT-LYS particles were obtained as CAT-LYS-1(1:1:1), CAT-LYS-2(2:1:1), CAT-LYS-3(1:2:1), and CAT-LYS-4(1:1:2) particles. The CAT-LYS-4 particles with the highest gravimetric yield of 68.9 +/- 6.0% and 783.6 +/- 56.6 nm hydrodynamic diameter was chosen for bioactivity studies. The CAT-LYS-4 particles exhibited 190.4 +/- 1.3 mu g/mL CAT-equivalent antioxidant capacity at 1000 mu g/mL concentration with TEAC value of 0.24 +/- 0.01 mu mole Trolox-equivalent/g antioxidant activity. They showed 16.81 +/- 3.47% Fe(II) chelation capacity at 350 mu g/mL and 185.8 +/- 22.8 mu mole Fe(III) reducing power at 500 mu g/mL concentration. Moreover, the CAT-LYS-4 particles retained more than half of the alpha-glucosidase inhibition activity of CAT in particulate form. Besides, a 50-fold improvement was achieved on the hemolytic blood compatibility of CAT-LYS-4 particles upon integration of LYS into CAT backbone (4.7 +/- 1.2% at 250 mu g/mL) compared to hemolysis ratio of native CAT molecules. They did not show coagulation effects up to 500 mu g/mL concentration with > 94% clotting indices. Hence, the CAT-LYS particles with enhanced blood compatibilities and well-retained inherent bioactivities of their precursors in 3D colloidal particulate structures can serve as natural biocolloids for drug/active molecule transport applications in biomedicine.
dc.description.sponsorshipScientific Research Commission of Canakkale Onsekiz Mart University [COMU FHD-2020-3310]
dc.description.sponsorshipThe authors gratefully acknowledge the financial support provided for this study by the Scientific Research Commission of Canakkale Onsekiz Mart University (COMU FHD-2020-3310).
dc.identifier.doi10.1007/s10965-022-02941-7
dc.identifier.issn1022-9760
dc.identifier.issn1572-8935
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85125339414
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1007/s10965-022-02941-7
dc.identifier.urihttps://hdl.handle.net/20.500.12428/23411
dc.identifier.volume29
dc.identifier.wosWOS:000757443800002
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofJournal of Polymer Research
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectCatechin
dc.subjectFlavonoid
dc.subjectLysine
dc.subjectAntioxidant
dc.subjectSelf-assembly
dc.subjectBlood compatible
dc.titleColloidal bioactive nanospheres prepared from natural biomolecules, catechin and L-lysine
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu