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Öğe Hyaluronic acid (HA)-Gd(III) and HA-Fe(III) microgels as MRI contrast enhancing agents(Elsevier Ltd, 2022) Şahiner, Nurettin; Umut, Evrim; Sağbaş Suner, Selin; Şahiner, Mehtap; Culha, Mustafa; Ayyala, Ramesh S.Hyaluronic acid (HA) was crosslinked with Gd(III) and Fe(III) ions rendering physically crosslinked HA-metal(III) microgels as magnetic resonance imaging (MRI) enhancing contrast agents. These HA-Gd(III) and HA-Fe(III) microgels are injectable with size range, 50–5000 nm in water. The same isoelectric point, pH 1.2 ± 0.1, was measured for both microgels. HA-Gd(III) and HA-Fe(III) microgels are hemo-compatible biomaterials and can be safely used in intravascular applications up to 1000 μg/mL concentration. Furthermore, no significant toxicity was attained as 95 ± 8 and 81 ± 2% cell viability on L929 fibroblast cells at 100 μg/mL of HA-Gd(III) and HA-Fe(III) microgels were measured. Moreover, HA-Gd(III) microgels were found to afford significant contrast improvement capability in MRI with proton relaxivity, r1 = 2.11 mM−1 s−1, comparable with the values reported for Gd(III) labeled functionalized HA gel systems and commercial Gd based contrast agents.Öğe Fabrication of Biodegradable Poly(naringin) Particles with Antioxidant Activity and Low Toxicity(Amer Chemical Soc, 2018) Şahiner, Mehtap; Şahiner, Nurettin; Sağbaş, Selin; Fullerton, Mitchell L.; Blake, Diane A.Naringin (NR, 4', 5,7 trihydrocyflavanone-7-O-rhamnoglucoside) is a flavanone found in citrus fruit that is composed of a phenolic compound, naringenin, and a disaccharide, neohesperidose. Poly(NR) [p(NR)] particles in the size range of few micrometers to few hundred nanometers were prepared from highly purified NR and subsequently characterized using UV/visible spectroscopy, Fourier transform infrared spectroscopy, nuclear magnetic resonance spectroscopy, scanning electron microscopy, thermogravimetric analysis, and zeta potential measurements. The hydrolytic degradation of p(NR) particles at 37.5 degrees C was investigated at pHs 5.4, 7.4, and 9.0. The particles degraded most rapidly at pH 7.4, with >90% degradation after 5 h. The cytotoxicity was assessed by growing COS-1 fibroblasts for 5 days in the presence of increasing concentrations of NR or eluates from the p(NR) particles. Both NR and p(NR) particles were nontoxic for these mammalian fibroblasts; at the highest concentration tested (571 mu g/mL), the percentages of surviving cells after 5 days in culture were 96.7 +/- 0.93 and 91 +/- 11% for NR and p(NR), respectively. The effect of p(NR) on red blood cell hemolysis was also negligible (<2%) at concentrations up to 100 mu g/mL; unpolymerized NR showed slightly higher levels of hemolysis than the p(NR) particles, but values never exceeded 5% at 50 and 100 mu g/mL. Both NR and p(NR) stimulated blood coagulation in a dose-dependent manner; the hemostatic effect was greater for p(NR), which, at 100 mu g/mL, stimulated in vitro blood clotting by similar to 50%. Neither NR nor eluates from p(NR) particles inhibited alpha-glucosidase activity; in fact, both provided a modest (10-30%) stimulation at concentrations from 0.67 to 1.67 mg/mL. p(NR) particles are easily synthesized, break down readily at physiological pH, and have excellent blood and biocompatibility. As such, they will be a useful carrier for drug delivery and as an oral antioxidant supplement.Öğe Responsive biopolymer-based microgels/nanogels for drug delivery applications(Woodhead Publ Ltd, 2018) Sağbaş Suner, Selin; Şahiner, Mehtap; Bütün Şengel, Sultan; Rees, Daniel J.; Reed, Wayne F.; Şahiner, NurettinÖğe Colloidal bioactive nanospheres prepared from natural biomolecules, catechin and L-lysine(Springer, 2022) Can, Mehmet; Şahiner, Mehtap; Şahiner, NurettinRecently, major attention has been devoted to exploring and increase biomedical applications of catechins by directly employing them as constituents of nano-vehicles. Here, (+)-catechin (CAT) was integrated with another benign biomolecule, L-Lysine (LYS) using formaldehyde (FA) via single-step Mannich condensation reaction and self-assembled supramolecular CAT-LYS networks were synthesized. By using various molar feed ratios of CAT, LYS, and FA (CAT:LYS:FA), different formulations of CAT-LYS particles were obtained as CAT-LYS-1(1:1:1), CAT-LYS-2(2:1:1), CAT-LYS-3(1:2:1), and CAT-LYS-4(1:1:2) particles. The CAT-LYS-4 particles with the highest gravimetric yield of 68.9 +/- 6.0% and 783.6 +/- 56.6 nm hydrodynamic diameter was chosen for bioactivity studies. The CAT-LYS-4 particles exhibited 190.4 +/- 1.3 mu g/mL CAT-equivalent antioxidant capacity at 1000 mu g/mL concentration with TEAC value of 0.24 +/- 0.01 mu mole Trolox-equivalent/g antioxidant activity. They showed 16.81 +/- 3.47% Fe(II) chelation capacity at 350 mu g/mL and 185.8 +/- 22.8 mu mole Fe(III) reducing power at 500 mu g/mL concentration. Moreover, the CAT-LYS-4 particles retained more than half of the alpha-glucosidase inhibition activity of CAT in particulate form. Besides, a 50-fold improvement was achieved on the hemolytic blood compatibility of CAT-LYS-4 particles upon integration of LYS into CAT backbone (4.7 +/- 1.2% at 250 mu g/mL) compared to hemolysis ratio of native CAT molecules. They did not show coagulation effects up to 500 mu g/mL concentration with > 94% clotting indices. Hence, the CAT-LYS particles with enhanced blood compatibilities and well-retained inherent bioactivities of their precursors in 3D colloidal particulate structures can serve as natural biocolloids for drug/active molecule transport applications in biomedicine.Öğe Functionalization of halloysite nanotubes with polyethyleneimine and various ionic liquid forms with antimicrobial activity(Wiley, 2020) Sağbaş Suner, Selin; Şahiner, Mehtap; Alper Akçalı; Şahiner, NurettinHalloysite nanotube (HNT), a natural clay, was modified with branched polyethyleneimine (PEI) to form PEI-HNT using epichlorohydrin (ECH) as coupling agent, then protonated with HCl to obtain H-PEI-HNTs providing [NH3](+)[Cl](-) functionality for potential antimicrobial properties. Upon PEI modification, zeta potential value of HNTs was increased to +37.3 mV from -34.5 mV and to +41.1 mV for H-PEI-HNTs. Only 1.87 wt % H-element in HNT was increased to 3.03 wt % upon PEI modification along with newly generated elements of N and C at 2.99 and 9.93 wt %, respectively. Moreover, ionic liquid (IL) forms of HNTs with [NH3](+)[N(CN)(2)](-), [NH3](+)[PF6](-) and [NH3](+)[BF4](-) functionality were generated via anion exchange of H-PEI-HNTs with sodium dicyanamide (SDC), ammonium hexafluorophosphate (AHFP), and sodium tetrafluoroborate (STFB). The antimicrobial properties of the modified, protonated, and IL forms of HNTs were determined via macro dilution, diffusion and agar screening tests against Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 10145, Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 6538 strains, and Candida albicans ATCC 10231 strains. It was found that H-PEI-HNTs possesses potent antimicrobial effect compared with the other forms of HNTs with 2-4 mg mL(-1) MIC and 8-16 mg mL(-1) MBC values via the macro dilution method. (c) 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 48352.Öğe Enhancement of biocompatibility and carbohydrate absorption control potential of rosmarinic acid through crossinking into microparticles(Elsevier, 2019) Şahiner, Mehtap; Blake, Diane A.; Fullerton, Mitchell L.; Sağbaş Suner, Selin; Sunol, Aydın K.; Şahiner, NurettinRosmarinic acid (RA), a bioflavonoid and antioxidant that exists in plants of the Lamiaceae family, was crosslinked into particles as poly(Rosmarinic Acid) (p(RA)) via an emulsion crosslinking method. The particles were characterized using scanning electron microscopy, Fourier transform infrared spectroscopy, solid state nuclear magnetic resonance C-13 NMR spectroscopy, and thermal gravimetric analysis. The zeta potential values of p(RA) particles were determined at different pHs; the isoelectric point was estimated as pH 1.2. The release of monomeric RA from the particles at 37.5 degrees C was found to be similar at different pHs, 1.0, 7.4, and 11.0. The effects of p(RA) on hemolysis and coagulation were found to be minimal. The antioxidant activity of p(RA) particles and RA monomer were almost indistinguishable suggesting that p(RA) particles may be used as an antioxidant. On a per weight basis, p(RA) particles were similar to 66% less cytotoxic to mammalian cells that RA monomer, as assessed using COS-1 cells. In addition, p(RA) was an 8.6-fold stronger inhibitor of alpha-glycosidase than RA; the IC(50)s of the monomer and particles were 0.121 and 0.014 mg/mL, respectively. The strong inhibitory effect of p(RA) on alpha-glycosidase, coupled with its reduced cytotoxicity and antioxidant activity, provide new opportunities for the use of p(RA). (C) 2019 Elsevier B.V. All rights reserved.Öğe Nitrogen-Doped Arginine Carbon Dots and Its Metal Nanoparticle Composites as Antibacterial Agent(Mdpi, 2020) Sağbaş Suner, Selin; Şahiner, Mehtap; Ayyala, Ramesh S.; Bhethanabotla, Venkat R.; Şahiner, NurettinNitrogen (N)-doped arginine carbon dots (Arg CD) were successfully synthesized using arginine as the amine source and citric acid as the carbon source via a one-pot green synthesis microwave-assisted technique in 2 min. Ag and Cu nanoparticles (NP) were generated within N-doped Arg CDs as composite Arg-Ag CDs and Arg-Cu CDs to render enhanced antibacterial properties. TEM analysis revealed that Arg CDs are in graphitic structures withdspacing ranging from 0.5 nm to 10 nm. The minimum inhibition concentration (MIC) values of Arg CDs with 6.250 mg/mL were decreased by about 100-fold for Arg-Ag CDs and ten-fold for Arg-Cu CDs with 0.062 and 0.625 mg/mL MIC values againstStaphylococcus aureus(S. aureus). The highest antibacterial susceptibility was observed for the Arg-Ag CD composite with 0.125 and 0.312 mg/mL minimum bactericidal concentration (MBC) values against Gram negativeS. aureusand Gram positiveEscherichia coli(E. coli) bacteria strains, respectively. It was found that the metal NPs within Arg CDs significantly increased the antibacterial properties of CDs making them available in the treatment of infections caused by different bacterial species. Furthermore, Arg-Ag CD and Arg-Cu CD composites were tested for Acetylcholinesterase (AChE, E.C. 3.1.1.7) that break down acetylcholine (ACh) into choline and acetic acid leading to the loss of ACh which plays an essential role as neurotransmitter in Alzheimer disease. It was found that Arg-Cu CDs inhibited 74.9 +/- 0.8% and Arg-Ag CDs inhibited 52.1 +/- 3.8% of AChE at a 1.82 mg/mL concentration versus no inhibition for Arg-CD. Moreover, the chelating activity of Arg-Cu CDs and Arg-Ag CDs were tested for Fe(II) and it was found that almost 100% chelating was attained at 116 mu g composites versus no measurable chelation for bare Arg CDs, suggesting the potential neurodegenerative disease treatment properties of these composite CDs in the brain.Öğe Yara Kaplama Malzemesi olarak Kollajen Esaslı Hidrojel Filmleri(2018) Şahiner, Mehtap; Sağbaş Suner, Selin; Turan, Abdullah; Erduğan, Hüseyin; Şahiner, NurettinYara kaplama malzemesi olarak kullanılacak kollajen/kitosan (koll/kitosan) vekollajen/kitosan/poli(N-izopropil akrilamid) (koll/kitosan/p(NIPAm)) iç içe geçmiş ağ yapılı(IPN) hidrojelleri gluteraldehit ve poli(etilenglikol)diakrilat çapraz bağlayıcıları kullanılarakfilm şeklinde sentezlenmiştir. Hazırlanan hidrojellerin morfolojik karakterizasyonu optikmikroskop ile yapılmış ve hidrojel yüzeylerinde 1-20 µm boyutunda mikro gözenekleriçerdiği belirlenmiştir. Hidrojellerin yapısal ve termal karakterizasyonu FT-IR spektroskopisive termal gravimetrik analiz (TGA) ölçümleri ile belirlenmiştir. Koll/kitosan vekoll/kitosan/p(NIPAm) IPN hidrojellerinin pH 5,4, 7,4 ve 9,0 da denge şişme kapasiteleribelirlenmiş ve koll/kitosan/p(NIPAm) hidrojeli en yüksek pH 5,4 değerinde % 428±97oranında şiştiği belirlenmiştir. Ayrıca hidrojellerin 25-50 ºC aralığındaki denge şişmedeğerleri ölçülmüş ve koll/kitosan hidrojellerinin yaklaşık % 200 şişme değeri ile neredeysebütün sıcaklıklarda aynı şişme derecesine sahip olduğu gözlemlenirkenkoll/kitosan/p(NIPAm) hidrojelinin 25 ºC sıcaklıkta % 312±14 oranında şişerken, yapısındakisıcaklık duyarlı p(NIPAM) den dolayı 50 ºC ısıtıldığında % 59±2 şişme değeri ile küçüldüğügözlemlenmiştir. Ayrıca, hazırlanan koll/kitosan esaslı IPN hidrojelleri deksametazon sodyumfosfat ilacının salımında kullanılmış ve koll/kitosan hidrojellerinin 114,6±2,9 mg/g ilacı 15saatte salarken koll/kitosan/p(NIPAm) aynı sürede yaklaşık 51,3±1,2 mg/g saldığıgözlemlenmiştir.Öğe Degradable natural phenolic based particles with micro-and nano-size range(Bentham Science Publishers B.V., 2018) Şahiner, Nurettin; Sağbaş, Selin; Şahiner, Mehtap; Aktaş, NahitBackground: Degradable polymeric particles derived from phenolic compounds are promising materials for biomedical applications due to their inherently antioxidant, antimicrobial, and anticancerogenic properties. We revise all the patent regarding to the biomedical and food additive formulations of Rutin (RT) and Quercetin (QC) as phenolic compounds. Objective: Prepare degradable Poly(Rutin) (p(RT)) and Poly(Quercetin) (p(QC)) particles from natural phenolic compounds, Rutin (RT) and Quercetin (QC). Method: P(RT), and p(QC) particles were prepared using microemulsion crosslinking method employing phenolic compounds such as RT and QC as monomer and poly(ethylene glycol) diglycidyl ether (PEGGE) as a crosslinker in a single step. The degradability of these particles was investigated at physiological conditions, pH 5.4, 7.4, and 9 at 37.5°C. The antioxidant capacity of RT, QC and their corresponding particles was determined by means of total phenol content and ABTS + scavenging assay. The blood compatibility of the particles is determined with hemolysis and blood clotting tests, and the cytotoxicity of the particles on L929 fibroblast cell and A549 cancer cells was done by WST-1 tests. Results: The size of the prepared phenolic particles was in the size range of 0.4-4 ?m with negative zeta potentials,-20.29±1.7 and-31.31±2.0 mV for p(RT) and p(QC) particles, respectively. The highest amount of degradation was obtained for p(QC) particles in almost a linear profile with relatively longer time degrading kinetics at pH 9, e.g., 197±23 mg/g QC was released up to 130 h. The antioxidant capacities of phenolic compounds were decreased about ten-fold upon the particle formations of the phenolic compound, and the antioxidant capacity of p(QC) particles was found to be better than p(RT) particles with 0.22±0.01 and 0.05±0.001 ?mol trolox equivalent g -1 , respectively. The blood compatibility test of p(RT) and p(QC) particles revealed that both particles are blood compatible up to 1 mg/mL concentration and possess clotting of blood over 1 mg/mL concentrations. Furthermore, the cytotoxicity tests showed that p(RT) particles are more biocompatible than p(QC) on the fibroblast cell as 91% cell viability versus 50% for p(QC) was observed at 75 ?g/mL particle concentrations. Additionally, at this concentration 42.3% of cancer cells were inhibited by p(RT) particles. Conclusion: Degradable p(RT) and p(QC) particles that are prepared in a single step offer great avenue for biomedical applications as highly antioxidant materials and with good biocompatibility in contact with blood and fibroblast cells, as well as great anticancerogenic capability against the cancer cells. © 2018 Bentham Science Publishers.Öğe Enhanced enzymatic activity and stability by in situ entrapment of α Glucosidase within super porous p(HEMA) cryogels during synthesis(Elsevier B.V., 2020) Demirci, Şahin; Şahiner, Mehtap; Yılmaz, Selehattin; Karadağ, Erdener; Şahiner, NurettinHere, poly(2-hydroxyethyl methacrylate) (p(HEMA)) cryogel were prepared in the presence 0.48, 0.96, and 1.92 mL of α-Glucosidase enzyme (0.06 Units/mL) solutions to obtain enzyme entrapped superporous p(HEMA) cryogels, donated as α-Glucosidase@p(HEMA)-1, α-Glucosidase@p(HEMA)-2, and α-Glucosidase@p(HEMA)-3, respectively. The enzyme entrapped p(HEMA) cryogels revealed no interruption for hemolysis and coagulation of blood rendering viable biomedical application in blood contacting applications. The α-Glucosidase@p(HEMA)-1 was found to preserve its’ activity% 92.3 ± 1.4 % and higher activity% against free α-Glucosidase enzymes in 15–60℃ temperature, and 4–9 pH range. The Km and Vmax values of α-Glucosidase@p(HEMA)-1 cryogel was calculated as 3.22 mM, and 0.0048 mM/min, respectively versus 1.97 mM, and 0.0032 mM/min, for free enzymes. The α-Glucosidase@p(HEMA)-1 cryogel was found to maintained enzymatic activity more than 50 % after 10 consecutive uses, and also preserved their activity more than 50 % after 10 days of storage at 25 ℃, whereas free α-Glucosidase enzyme maintained only 1.9 ± 0.9 % activity under the same conditions.Öğe Biomedical applications of nanofibers(wiley, 2022) Şahiner, Mehtap; Kurt, Saliha B.; Şahiner, NurettinElectrospinning is one of the most convenient and effective techniques in the production of fibers that employs high voltage. The tunable parameters of the electrospinning technique, e.g. flow rate of the polymer solution, voltage, distance between needle and the collector, and viscosity of polymer solution, allow fibers with different sizes and characteristics to be produced, i.e. micro-to nanosized fibers with different types and extent of porosity. Electrospun fibers are well suited for a broad range of applications including environmental, energy, biotechnology, health care, and biomedical applications. Recently, electrospun fibers have attracted great interest for drug delivery, wound healing, and biosensor applications in the biomedical field. The electrospinning technique is attractive due to its simplicity and low cost and is widely employed in both academia and industry. In this chapter, the utilization of the electrospinning technique in the fabrication of nanofibers from various natural and synthetic polymers and their biomedical applications will be discussed. © 2022 John Wiley & Sons, Inc.Öğe Hydrolytic nondegradable bioactive rosmarinic acid particles(John Wiley and Sons Ltd, 2021) Şahiner, MehtapRosmarinic acid (RS) is as the nonflavonoid polyphenols in the phenolic acid subgroup was cross-linked with sodium trimetaphosphate (STMP) to obtain (p[RS-co-STMP]) particles with the size distribution of 2.992 ± 659 nm. The zeta potential values of p(RS-co-STMP) particles were measured between pH 2–10, and the isoelectric point was determined as pH 2.66. Fe(II) chelating capability test was done for RS and p(RS-co-STMP). At 800 μmol/ml concentrations, p(RS-co-STMP) particles chelated 95.06 ± 5.18% Fe(II), while RS molecule did not chelate Fe(II), whereas STMP chelated only 41.8 ± 5.9% Fe(II). The effects of RS and p(RS-co-STMP) particles on α-glucosidase enzyme activity were investigated and were found to inhibit the α-glucosidase enzyme by 55.7% and 89.6%, respectively. Furthermore, p(RS-co-STMP) particles were modified with polyethyleneimine as m-p(RS-co-STMP) to improve antimicrobial properties and found effective against both Escherichia coli and Staphylococcus aureus bacteria. The interaction of fibrinogen with RS, p(RS-co-STMP) and m-p(RS-co-STMP) were studied via the change in intensity of corresponding fluorescence spectra. It was found that p(RS-co-STMP) particles interacted lesser with fibrinogen than RS and changed the fluorescence property of fibrinogen protein slightly. On the other hand, m-p(RS-co-STMP) particles did not change the fluorescence intensity of fibrinogen suggesting no influence on the blood clotting.Öğe Chondroitin Sulfate-Based Cryogels for Biomedical Applications(MDPI, 2021) Demirci, Şahin; Şahiner, Mehtap; Ari, Betül; Sunol, Aydın K.; Şahiner, NurettinCryogels attained from natural materials offer exceptional properties in applications such as tissue engineering. Moreover, Halloysite Nanotubes (HNT) at 1:0.5 weight ratio were embedded into CS cryogels to render additional biomedical properties. The hemolysis index of CS cryogel and CS:HNT cryogels was calculated as 0.77 ± 0.41 and 0.81 ± 0.24 and defined as non-hemolytic mate-rials. However, the blood coagulation indices of CS cryogel and CS:HNT cryogels were determined as 76 ± 2% and 68 ± 3%, suggesting a mild blood clotting capability. The maximum% swelling capacity of CS cryogel was measured as 3587 ± 186%, 4014 ± 184%, and 3984 ± 113%, at pH 1.0, pH 7.4 and pH 9.0, respectively, which were reduced to 1961 ± 288%, 2816 ± 192, 2405 ± 73%, respectively, for CS:HNT cryogel. It was found that CS cryogels can hydrolytically be degraded 41 ± 1% (by wt) in 16-day incubation, whereas the CS:HNT cryogels degraded by 30 ± 1 wt %. There is no chelation for HNT and 67.5 ± 1% Cu(II) chelation for linear CS was measured. On the other hand, the CS cryogel and CS:HNT cryogel revealed Cu(II) chelating capabilities of 60.1 ± 12.5%, and 43.2 ± 17.5%, respectively, from 0.1 mg/mL Cu(II) ion stock solution. Additionally, at 0.5 mg/mL CS, CS:HNT, and HNT, the Fe(II) chelation capacity of 99.7 ± 0.6, 86.2 ± 4.7% and only 11.9 ± 4.5% were measured, respectively, while no Fe(II) was chelated by linear CS chelated Fe(II). As the adjustable and con-trollable swelling properties of cryogels are important parameters in biomedical applications, the swelling properties of CS cryogels, at different solution pHs, e.g., at the solution pHs of 1.0, 7.4 and 9.0, were measured as 3587 ± 186%, 4014 ± 184%, and 3984 ± 113%, respectively, and the maximum selling% values of CS:HNT cryogels were determined as 1961 ± 288%, 2816 ± 192, 2405 ± 73%, re-spectively, at the same conditions. Alpha glucosidase enzyme interactions were investigated and found that CS-based cryogels can stimulate this enzyme at any CS formulation.Öğe Rod-like L-Aspartic acid-Cu(II) metal organic frameworks; Synthesis, characterization and biomedical properties(Elsevier B.V., 2021) Gizer, Görkem; Şahiner, Mehtap; Yıldırım, Yıldız; Demirci, Şahin; Can, Mehmet; Şahiner, NurettinL-aspartic acid (L-AA) based MOFs were prepared from acetate, chloride, nitrate, and sulfate salts of Cu(II) ions as L-AA-Cu(II)-A,-C,-N,-S, respectively with 96.7 2.4, 89.8 3.1, 92.1 1.5, 74.6 5.2 m2/g, surface areas. L-AA- Cu(II)-MOFs in the same order induced 0.43 0.25%, 0.94 0.24%, 0.91 0.40%, 1.18 0.10% hemolysis, all being <2%, and blood clotting indices of ~90% and can be considered nonhemolytic and non-coagulative at 1 μg/mL concentration. L-AA-Cu(II)-A MOFs exhibited 86.3 0.2, and 92.4 0.6% α-Glucosidase inhibitory ac- tivities at 1.0 and 10.0 μg/mL concentrations, respectively. Moreover, L-AA-Cu(II)-S MOFs had effective anti- microbial activities against E. coli (ATCC-8739), and S. aureus (ATCC-6538) with MIC values of 0.63 mg/mL and 1.25 mg/mL for C. albicans (ATCC-10231). L-AA-Cu(II) MOFs synthesized herein with hemocompatible, antimi- crobial and antidiabetic properties prompt interesting possibilities for both industrial and biomedical applicationsÖğe Biodiverse Properties of Tannic Acid-Based Fibers(Springer, 2021) Şahiner, Mehtap; Kurt, Saliha B.; Şahiner, NurettinTannic acid (TA) is a plant-based polyphenolic molecule with enticing anti-oxidant, anti-bacterial, antiinflammatory and anti-cancer features, making it a valuable material in bio-medicinal applications. To establish whether TA-based fibers are useful tools in potential medical textiles, e.g., as wound dressing material for prophylaxis against infections and diseases, TA fibers were prepared and employed in biological assays. TA fibers were prepared with 55 weight% TA in ethanol-DI water mixture and fabricated by the electrospinning technique. Bare TA-based fibers were examined and were 1.2 +/- 0.1 % non-hemolytic and had 8.7 +/- 1.7 blood clotting index up to 2 mg/ml concentration. Degradation of bare TA-based fibers was completed in 5 minutes; however, degradation of crosslinked TA-based fibers were 98.3 +/- 2.3 % and 83.1 +/- 5.4 % for TA-Ibu and TA in 168 hours. Anti-oxidant activity of TA-based fibers was investigated by TEAC, total phenol content (TPC) and total flavonoid content (TFC) assays. Bare TA fibers possessed the highest anti-oxidant activity of 5.7 +/- 0.5 mM trolox equivalent/g, 168.0 +/- 2.0 gallic acid equivalent mu g/ml and 193.0 +/- 17.0 quercetin equivalent mu g/ml. Anti-bacterial activity was investigated by the disc diffusion method and the highest inhibition zone diameter was measured as 3.5 +/- 0.2 cm against S. aureus (gram +) bacteria; however, the same fiber was detected as producing 2.2 +/- 0.5 cm zone diameter for E. coli (gram -) bacteria.
