A single-use electrochemical biosensor system for ultrasensitive detection of Aflatoxin B1 in rice, corn, milk, peanut, chili pepper samples

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dc.authoridUZMAN, ECEM/0009-0007-3714-2589
dc.contributor.authorDemirbakan, Burcak
dc.contributor.authorKoseer, Nur Tarimeri
dc.contributor.authorUzman, Ecem
dc.contributor.authorÖzay, Özgür
dc.contributor.authorOzay, Hava
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2025-01-27T20:17:16Z
dc.date.available2025-01-27T20:17:16Z
dc.date.issued2024
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractAflatoxin B1, a common food contaminant in peanuts and corn and a genotoxic carcinogen in humans poses a significant risk for hepatocellular carcinoma, making its detection crucial; this study aims to develop a label-free electrochemical biosensor using a disposable indium tin oxide polyethylene terephthalate (ITO-PET) electrode modified with 3-Aminopropyltriethoxysilane for detecting Aflatoxin B1 in real food samples. Initially, optimization steps for the proposed biosensor were conducted using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques. Characterization steps such as storage capacity, regeneration, and single frequency impedance (SFI) were completed for the proposed disposable biosensor after the optimization steps. The electrochemical biosensor, based on AFB1, exhibited excellent repeatability and reproducibility. It had a broad dynamic detection range from 0.1 fg/mL to 500 fg/mL, with low limits of detection (LOD) and quantitation (LOQ) at 0.19 fg/mL and 0.65 fg/mL, respectively. Finally, the proposed AFB1-based biosensor system was applied to real food samples (rice, chili pepper, milk, corn, and peanuts) for testing and validation.
dc.description.sponsorshipanakkale Onsekiz Mart University Scientific Research Projects Coordination Unit [FBA-2020-3431]
dc.description.sponsorshipThis work was supported by the Canakkale Onsekiz Mart University Scientific Research Projects Coordination Unit, Project number: FBA-2020-3431.
dc.identifier.doi10.1016/j.jfca.2024.106701
dc.identifier.issn0889-1575
dc.identifier.issn1096-0481
dc.identifier.scopus2-s2.0-85203440049
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.jfca.2024.106701
dc.identifier.urihttps://hdl.handle.net/20.500.12428/21534
dc.identifier.volume136
dc.identifier.wosWOS:001313831500001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherAcademic Press Inc Elsevier Science
dc.relation.ispartofJournal of Food Composition and Analysis
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectAflatoxin B1
dc.subject3-Aminopropyltriethoxysilane
dc.subjectBiosensor
dc.subjectFood contamination
dc.titleA single-use electrochemical biosensor system for ultrasensitive detection of Aflatoxin B1 in rice, corn, milk, peanut, chili pepper samples
dc.typeArticle

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