A Label-free Electrochemical Immunosensor for Highly Sensitive Detection of TNF alpha, Based on Star Polymer-modified disposable ITO Electrode

Basit Öğe Kaydı
dc.authorid0000-0003-3042-1087en_US
dc.authorscopusid6506764918en_US
dc.authorwosidAAY-1439-2021en_US
dc.contributor.authorAydın, Elif Burcu
dc.contributor.authorAydın, Muhammet
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2023-07-24T12:44:39Z
dc.date.available2023-07-24T12:44:39Z
dc.date.issued2021en_US
dc.departmentFakülteler, Mühendislik Fakültesi, Biyomühendislik Bölümü
dc.description.abstractBackground: Biomarkers are very important disease-related biomolecules which should be analyzed sensitive and selective in related physiological fluids or tissues. Tumor necrosis factor-α is a type of cytokine which plays vitlly important roles in different methabolic pathways such as cell death, survival, differentiation, proliferation and migration, and infectious and inflammatory diseases including rheumatoid arthritis, diabetes. Objective: In this study, it was aimed to develop a reliable tool based on star-shaped poly(glycidyl methacrylate) polymer coated disposable indium tin oxide electrode for determination of Tumor necrosis factor-α, an important disease biomarker. Methods: Star shaped polymer was used as an interface material for anti-Tumor necrosis factor α antibodies immobilization. The antibodies were immobilized covalently onto polymer coated indium tin oxide electrode. Electrochemical impedance spectroscopy and cyclic voltammetry techniques were used for all electrochemical measurements. Results: The suggested immunosensor exhibited a linear range between 0.02 and 4 pg/mL Tumor necrosis factor-α, and the detection limit was found as 6 fg/mL. Scanning electron microscopy and atomic force microscopy were used for electrode surface characterization. In addition, the suggested im-munosensor was used for Tumor necrosis factor-α sensing in human serum samples. The results dis-played recoveries between 97.07 and 100.19%. Moreover, this immunosensor had a simple fabrication procedure and a long storage-stability. Conclusion: A new biosensor based on a Star shaped polymer for the ultra sensitive determination of a biomarker Tumor necrosis factor-α was developed. The biosensor presented excellent repeatability and reproducubility, and also wide calibration range for Tumor necrosis factor-α.en_US
dc.identifier.citationAydın, E. B., Aydın, M., & Sezgintürk, M. K. (2021). A label-free electrochemical immunosensor for highly sensitive detection of TNF α, based on star polymer-modified disposable ito electrode. Current Pharmaceutical Analysis, 17(3), 450-459. doi:10.2174/1573412916999200409111759en_US
dc.identifier.doi10.2174/1573412916999200409111759
dc.identifier.endpage459en_US
dc.identifier.issn1573-4129
dc.identifier.issn1875-676X
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85099702364
dc.identifier.startpage450en_US
dc.identifier.urihttps://doi.org/10.2174/1573412916999200409111759
dc.identifier.urihttps://hdl.handle.net/20.500.12428/4441
dc.identifier.volume17en_US
dc.identifier.wosWOS:000614254400010
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorSezgintürk, Mustafa Kemal
dc.language.isoen
dc.publisherBentham Science Publishersen_US
dc.relation.ispartofCurrent Pharmaceutical Analysisen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectElectrochemical immunosensoren_US
dc.subjectSingle frequencies impedanceen_US
dc.subjectStar poly(glycidyl methac-rylate) polymeren_US
dc.subjectTumor necrosis factor αen_US
dc.titleA Label-free Electrochemical Immunosensor for Highly Sensitive Detection of TNF alpha, Based on Star Polymer-modified disposable ITO Electrode
dc.typeArticle

Dosyalar

Lisans paketi
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Biyomühendislik Bölümü Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu