The effect of vitamin E on mast cells in small intestine of broilers under heat stress
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Tarih
2017
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Hellenic Veterinary Medical Soc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The aim of this study is to identify the effect of vitamin E (DL-alpha-tocopherol acetate) (300 IU/kg) on mast cells in the small intestine (duodenum, jejunum and ileum) under heat stress. In the study, 42 one-day-old Ross 308 male broiler chicks were used. The chicks were randomly separated into 3 groups as follows; control (22 +/- 2 degrees C), heat stress (35 degrees C, 5 hours/per day) and vitamin E (300 IU/kg/per day) + heat stress (35 degrees C, 5 hours/per day). The applications of heat stress and vitamin E began on the fifteenth day and ended on the thirty-fifth day. Tissue samples were taken from animals in each group of four and five-week-old chickens. Tissue samples were fixed in BLA (Basic Lead Acetate) solution. The sections were stained with toluidine blue (TB) (pH 0.5) and alcian blue-critical electrolyte concentration (AB-CEC) (pH 5.8, 0.3 M MgCl2) / Safranin O (SO) (pH 1.0) combined method. It was determined that increasing of the exposure duration to heat stress increased the number of mast cells in the small intestine of the boilers. Also, it was revealed that vitamin E reduced mast cell population under heat stress. Consequently, heat stress may play a role in the pathogenesis of small intestine-associated with disorders and the supplementation of vitamin E can contribute to regulate small intestine functions of broilers by decreasing mast cell proliferation and activation under heat stress.
Açıklama
Anahtar Kelimeler
Broiler, heat stress, mast cell, vitamin E
Kaynak
Journal of The Hellenic Veterinary Medical Society
WoS Q Değeri
Q4
Scopus Q Değeri
Q4
Cilt
68
Sayı
2
