Genotoxic and cytotoxic effects of polyethylene microplastics on human peripheral blood lymphocytes

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dc.authorid0000-0001-9640-3354en_US
dc.authorid0000-0002-2014-6635en_US
dc.authorscopusid57195554629en_US
dc.authorscopusid22733342800en_US
dc.authorwosid-en_US
dc.authorwosid-en_US
dc.contributor.authorÇobanoğlu, Hayal
dc.contributor.authorBelivermiş, Murat
dc.contributor.authorSıkdokur, Ercan
dc.contributor.authorKılıç, Önder
dc.contributor.authorÇayır, Akın
dc.date.accessioned2024-12-23T12:23:14Z
dc.date.available2024-12-23T12:23:14Z
dc.date.issued2021en_US
dc.departmentMeslek Yüksekokulları, Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Hizmetler ve Teknikler Bölümü
dc.description.abstractCurrently, we need emerging initial data regarding how plastic exposures affect cellular and molecular components and how such interactions will be crucial for human health. We aimed to determine the genotoxic and cytotoxic effects of microplastic (MPs,10-45 μm, polyethylene) on human peripheral lymphocytes by using the cytokinesis-block micronucleus cytome (CBMN) assay, which is a comprehensive method to reveal a range of mechanisms, not only diseases but also response to environmental exposures. We measured micronucleation (MN), nucleoplasmic bridge formation (NPB), and nuclear bud formation (NBUD) in human peripheral blood lymphocytes. We also measured the cytokinesis-block proliferation index (CBPI) to calculate cytostasis, which indicates cytotoxicity in lymphocytes treated with five different MPs concentrations for 48 h. Even lower concentrations of MPs increased the level of genomic instability. We found that the in vitro MP exposure significantly increased MN, NPB, and NBUD frequencies. Since we investigated the effect of larger particles relative to the lymphocytes, mechanic interaction of MPs with cells, the release of monomer and additives from MPs could be suggested as possible mechanisms accounting for increasing genomic instabilities. We did not observe a decrease in the cell proliferation index, indicating a lack of MPs’ cytotoxic potential. To the best of our knowledge, our study is the first to identify MPs’ genotoxic potential in human peripheral blood lymphocytes. We suggested further studies to investigate the genotoxic and cytotoxic potential of smaller plastics and the chronic effect of MP on the human population.en_US
dc.identifier.citationÇobanoğlu, H., Belivermiş, M., Sıkdokur, E., Kılıç, Ö., & Çayır, A. (2021). Genotoxic and cytotoxic effects of polyethylene microplastics on human peripheral blood lymphocytes. Chemosphere, 272, 129805. https://doi.org/10.1016/j.chemosphere.2021.129805en_US
dc.identifier.doi10.1016/j.chemosphere.2021.129805
dc.identifier.issn0045-6535
dc.identifier.issn1879-1298
dc.identifier.pmid35534956
dc.identifier.scopus2-s2.0-85100897387
dc.identifier.urihttps://doi.org/10.1016/j.chemosphere.2021.129805
dc.identifier.urihttps://hdl.handle.net/20.500.12428/6781
dc.identifier.volume272en_US
dc.identifier.wosWOS:000635594700057
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorÇobanoğlu, Hayal
dc.institutionauthorÇayır, Akın
dc.language.isoen
dc.publisherElsevier Ltden_US
dc.relation.ispartofChemosphereen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCytotoxicityen_US
dc.subjectDNA Damageen_US
dc.subjectGenotoxicityen_US
dc.subjectMicronucleusen_US
dc.subjectMicroplasticsen_US
dc.subjectNBUDen_US
dc.subjectNPBen_US
dc.subjectPolyethyleneen_US
dc.titleGenotoxic and cytotoxic effects of polyethylene microplastics on human peripheral blood lymphocytes
dc.typeArticle

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