Exposure to glyphosate and tetrachlorvinphos induces cytotoxicity and global DNA methylation in human cells
Yükleniyor...
Tarih
2021
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
SAGE Publications
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Two organophosphate pesticides—glyphosate and tetrachlorvinphos—have been announced as carcinogens to
humans by various authorities, including the European Chemical Agency and the Environmental Protection
Agency. We aimed to investigate molecular mechanisms associated with carcinogenicity and to examine changes
in global m5
C DNA methylation and cytotoxic potential in A549 lung epithelial cells in response to glyphosate
and tetrachlorvinphos, and differential gene expression of m5
C DNA methyltransferase genes in Sprague
Dawley rats to Roundup (commercial formulation of glyphosate). Global m5
C level significantly increased after
1500 μM glyphosate exposure for 24 h. We determined that exposure to tetrachlorvinphos did not significantly
increase the m5
C level in A549 cells for 24 h. Additionally, we did not observe significant DNA methylation
alteration for both pesticides after 12 h exposure. In the animal study, we observed that DNA methyltransferase
genes (DNMT3b and DNMT3a) showed significantly higher expression in Roundup-exposed rats than the
control group in the liver and kidney. We also observed that a significant cytotoxic effect was determined after
the treatment of the cells with higher concentrations of glyphosate and tetrachlorvinphos. Our results revealed
that DNA methylation could be modified by exposure to glyphosate and that exposure to Roundup was
associated with the differential expression level of m5
C DNA methylation methyltransferase. Finally, exposure
to both pesticides increased cytotoxicity.
Açıklama
Anahtar Kelimeler
DNA Methylation, Organophosphate Pesticide, Glyphosate, Tetrachlorvinphos, Gene Expression
Kaynak
Toxicology and Industrial Health
WoS Q Değeri
Q3
Scopus Q Değeri
Cilt
37
Sayı
10
Künye
Ergun, H., & Cayir, A. (2021). Exposure to glyphosate and tetrachlorvinphos induces cytotoxicity and global DNA methylation in human cells. Toxicology and Industrial Health, 37(10), 610-618. doi:10.1177/07482337211033149
