Early and sensitive diagnosis of celiac autoimmune disease by using carboxylic acid functionalized magnetic nanoparticles-assisted biosensing platform

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dc.contributor.authorAydin, Elif Burcu
dc.contributor.authorAydin, Muhammet
dc.contributor.authorSezginturk, Mustafa Kemal
dc.date.accessioned2025-05-29T02:58:07Z
dc.date.available2025-05-29T02:58:07Z
dc.date.issued2025
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractA novel impedimetric magneto-immunosensor based on iron oxide (Fe3O4) nanoparticles coated with 3-phosphonopropionic acid (3-PPA) (functionalized magnetic beads, or FMBs) was created for the highly sensitive and selective detection of anti-tissue transglutaminase antibody (anti-tTG) in human serum. This label-less immunosensor was introduced by magnetically attaching FMBs onto the working electrode surface with a neodium magnet. The FMBs were utilized as a sensing interface and had carboxylic acid groups for tTG molecules, which could selectively link the target anti-tTG antibody. The FMBs modification steps were carried out in microcentrifuge tubes and concentrated with magnetic force before electrochemical analyses. The specific immuno-interactions on the FMBs surface were characterized by using the electrochemical and microscopic techniques, and in the presence of anti-tTG antibodies, they were captured by tTG-immobilized magnetic beads, and significant increases were observed in impedimetric response. The magneto biosensor response was linearly related to the anti-tTG antibody level in a broad linear range of 0.125-15.62 U/mL and a low detection limit (LOD) of 0.04 U/mL. Additionally, this magneto sensor was stable, repeatable, reproducible, selective, and sensitive for determination of the anti-tTG. The commercial enzyme-linked immunosorbent assay (ELISA) method was employed to compare the responses of the suggested immunosensor in actual samples. The magneto biosensor results were in good agreement with the ELISA reference technique results. Consequently, the biosensor performance in the analysis of serum samples was acceptable.
dc.description.sponsorshipScientific and Technological Research Council of Tekirdag Namik Kemal University [NKUBAP.00. GA.23.465]; Scientific and Technological Research Council of Turkiye (TUBITAK)
dc.description.sponsorshipThis project was supported by the Scientific and Technological Research Council of Tekirdag Namik Kemal University (NKUBAP.00. GA.23.465).Open access funding provided by the Scientific and Technological Research Council of Turkiye (TUBITAK).
dc.identifier.doi10.1007/s00604-025-07129-6
dc.identifier.issn0026-3672
dc.identifier.issn1436-5073
dc.identifier.issue5
dc.identifier.pmid40198415
dc.identifier.scopus2-s2.0-105002965235
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1007/s00604-025-07129-6
dc.identifier.urihttps://hdl.handle.net/20.500.12428/30281
dc.identifier.volume192
dc.identifier.wosWOS:001463088300007
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer Wien
dc.relation.ispartofMicrochimica Acta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250529
dc.subjectAnti-tissue transglutaminase antibody
dc.subjectMagneto biosensor
dc.subject3-phosphonopropionic acid
dc.subjectMagnetic beads
dc.titleEarly and sensitive diagnosis of celiac autoimmune disease by using carboxylic acid functionalized magnetic nanoparticles-assisted biosensing platform
dc.typeArticle

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