Electrocatalytic oxidation of NADH using a pencil graphite electrode modified with quercetin

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dc.authoridDilgin, Yusuf/0000-0002-2980-6871
dc.authoridGiray Dilgin, Didem/0000-0002-3487-3470
dc.contributor.authorDilgin, Yusuf
dc.contributor.authorKizilkaya, Bayram
dc.contributor.authorDilgin, Didem Giray
dc.contributor.authorGokcel, H. Ismet
dc.contributor.authorGorton, Lo
dc.date.accessioned2025-01-27T20:12:19Z
dc.date.available2025-01-27T20:12:19Z
dc.date.issued2013
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn the present study, the electrocatalytic oxidation of reduced beta nicotinamide adenine dinucleotide (NADH) was investigated using a pencil graphite electrode modified with quercetin (PGE/QH(2)). The PGE/QH(2) was prepared through two steps: (i) the pre-treatment of PGE at 1.40V vs. Ag vertical bar AgCl vertical bar KCl(sat.) in pH 7.0 phosphate buffer containing 0.1 M KCl for 60 s and (ii) adsorption of QH(2) on the PGE via immersion of PGE into a 1.0 mM QH(2) solution (in ethanol) for 60 s. Cyclic voltammetric studies show that the peak potential of NADH oxidation shifts from +500 mV at bare PGE to +300 mV at PGE/QH(2). The electrocatalytic currents obtained from amperometric measurements at +300 mV vs. Ag vertical bar AgCl vertical bar KCl(sat.) and in phosphate buffer solution at pH 7.0 containing 0.1 M KCl were linearly related to the concentration of NADH. Linear calibration plots are obtained in the concentration range from 0.5 mu M to 100 mu M. The limit of detection was found to be 0.15 mu M. Crown Copyright (c) 2012 Published by Elsevier B.V. All rights reserved.
dc.identifier.doi10.1016/j.colsurfb.2012.09.030
dc.identifier.endpage821
dc.identifier.issn0927-7765
dc.identifier.issn1873-4367
dc.identifier.pmid23107961
dc.identifier.scopus2-s2.0-84867772909
dc.identifier.scopusqualityQ1
dc.identifier.startpage816
dc.identifier.urihttps://doi.org/10.1016/j.colsurfb.2012.09.030
dc.identifier.urihttps://hdl.handle.net/20.500.12428/20911
dc.identifier.volume102
dc.identifier.wosWOS:000313301200116
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofColloids and Surfaces B-Biointerfaces
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectElectrocatalytic oxidation
dc.subjectModified electrode
dc.subjectQuercetin
dc.subjectNADH
dc.subjectPencil graphite electrode
dc.titleElectrocatalytic oxidation of NADH using a pencil graphite electrode modified with quercetin
dc.typeArticle

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