Electrocatalytic oxidation of NADH using a pencil graphite electrode modified with quercetin
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Tarih
2013
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In the present study, the electrocatalytic oxidation of reduced beta nicotinamide adenine dinucleotide (NADH) was investigated using a pencil graphite electrode modified with quercetin (PGE/QH(2)). The PGE/QH(2) was prepared through two steps: (i) the pre-treatment of PGE at 1.40V vs. Ag vertical bar AgCl vertical bar KCl(sat.) in pH 7.0 phosphate buffer containing 0.1 M KCl for 60 s and (ii) adsorption of QH(2) on the PGE via immersion of PGE into a 1.0 mM QH(2) solution (in ethanol) for 60 s. Cyclic voltammetric studies show that the peak potential of NADH oxidation shifts from +500 mV at bare PGE to +300 mV at PGE/QH(2). The electrocatalytic currents obtained from amperometric measurements at +300 mV vs. Ag vertical bar AgCl vertical bar KCl(sat.) and in phosphate buffer solution at pH 7.0 containing 0.1 M KCl were linearly related to the concentration of NADH. Linear calibration plots are obtained in the concentration range from 0.5 mu M to 100 mu M. The limit of detection was found to be 0.15 mu M. Crown Copyright (c) 2012 Published by Elsevier B.V. All rights reserved.
Açıklama
Anahtar Kelimeler
Electrocatalytic oxidation, Modified electrode, Quercetin, NADH, Pencil graphite electrode
Kaynak
Colloids and Surfaces B-Biointerfaces
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
102
