Effects of Donor x Inducer Interaction on the Success of Haploid Induction and Comparison of Haploid Seed Identification Methods in the In vivo Maternal Haploid Technique in Maize

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dc.contributor.authorKahrıman, Fatih
dc.contributor.authorSongur, Umut
dc.contributor.authorDisbudak, Abdullah
dc.contributor.authorKizik, Sezgin
dc.contributor.authorVural, Berk
dc.date.accessioned2025-01-27T20:47:21Z
dc.date.available2025-01-27T20:47:21Z
dc.date.issued2022
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThis study was conducted to investigate the effect of donor and inducer lines on the haploid induction rate in the in vivo maternal doubled haploid technique in maize and to compare the methods used in haploid seed separation. In the study, three donor materials were used for induction crossing with two inducer lines. Seedling immersion and stem injection techniques were used as chromosome doubling methods. Eye separation, spectral measurements and image processing were used to identify seed classes. Data were modeled using support vector machine method and created models were evaluated over the complexity matrix. The results of the research revealed that the haploid induction rate varies depending on the donor genotypes and inducers, and the genotype responses against the applied chromosome doubling method were also different. Differences were observed in the success of the three methods compared for haploid and diploid seed separation. The successful classification rates were 87%, 83%, and 79% in visual, spectral and image processing models, respectively. Results showed that both spectral technique and image processing technique can be used to distinguish haploid/diploid seeds in in vivo maternal haploid technique.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [119O619]
dc.description.sponsorshipThis work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) (Project number:119O619) The authors thank TUBITAK for their financial support.
dc.identifier.doi10.15832/ankutbd.824114
dc.identifier.endpage395
dc.identifier.issn1300-7580
dc.identifier.issn2148-9297
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85137911870
dc.identifier.scopusqualityQ3
dc.identifier.startpage385
dc.identifier.trdizinid1138125
dc.identifier.urihttps://doi.org/10.15832/ankutbd.824114
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1138125
dc.identifier.urihttps://hdl.handle.net/20.500.12428/24857
dc.identifier.volume28
dc.identifier.wosWOS:000877379000004
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherAnkara Univ, Fac Agriculture
dc.relation.ispartofJournal of Agricultural Sciences-Tarim Bilimleri Dergisi
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectCite this article
dc.subjectSeed classification
dc.subjectSpectral analysis
dc.subjectPloidy
dc.subjectChromosome doubling
dc.subjectSupport vector machine
dc.titleEffects of Donor x Inducer Interaction on the Success of Haploid Induction and Comparison of Haploid Seed Identification Methods in the In vivo Maternal Haploid Technique in Maize
dc.typeArticle

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