Estimation of arsenic-induced genotoxicity in melon (Cucumis melo) by using RAPD-PCR and comet assays
Yükleniyor...
Tarih
2021
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
In this study, arsenic (As)-induced genotoxicity in the roots and shoots of Cucumis melo (melon) seedlings were investigated by using the random amplified polymorphic DNA - polymerase chain reaction (RAPD-PCR) and comet assays. For this purpose, melon seedlings were exposed to arsenate [As(V)] at 0, 100, 200, 300, and 400 μM concentrations in the hydroponic system for 14 days to examine the level of As accumulation, alterations in growth parameters, and DNA damage. A reduction in growth with increasing As(V) concentration was observed in the melon seedlings. Total As accumulations in the shoot and root tissue increased in a dose-dependent manner; however, the level was higher in the roots than the shoots. In RAPD-PCR analysis, 26 primers gave reproducible and scorable results and produced a total of 128 bands in the control seedlings. Alterations in RAPD profiles, including the loss or appearance of new bands, were determined in the As-treated seedlings when compared to the control. The values of genomic template stability (GTS) were decreased by increasing the concentration of the As(V) in both tissue types. DNA strand breaks were observed in all the tested As(V) concentrations in the alkaline comet assay; furthermore, the loss of DNA integrity was higher with 300 and 400 μM As(V) treatments. The results clearly indicate that the combination of DNA-based molecular and cytogenetic techniques (e.g. the comet assay) may be proposed as a reliable evaluation of genotoxicity in plants after exposure to heavy metal pollution.
Açıklama
Anahtar Kelimeler
DNA damage, Genomic stability, Genotoxicity tests, Heavy metal stress
Kaynak
Botanica Serbica
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
45
Sayı
1
Künye
Surgun-Acar, Y. (2021). Estimation of arsenic-induced genotoxicity in melon (cucumis melo) by using RAPD-PCR and comet assays. Botanica Serbica, 45(1), 97-106. doi:10.2298/BOTSERB2101097S
