Bioengineered three-dimensional physical constructs from quince seed mucilage for human adipose-derived mesenchymal stem cells

Basit Öğe Kaydı
dc.authoridŞimşek, Ekin / 0000-0002-8501-7746
dc.authoridArslan, Yavuz Emre / 0000-0003-3445-1814
dc.contributor.authorŞimşek, Ekin
dc.contributor.authorKaraca, Burak
dc.contributor.authorArslan, Yavuz Emre
dc.date.accessioned2025-01-27T20:56:10Z
dc.date.available2025-01-27T20:56:10Z
dc.date.issued2020
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn this study, we aimed at fabricating a novel porous physical construct from quince seed mucilage for translational medicine applications. To achieve this goal, quince seed mucilage was extracted, molded, and freeze-dried. After being freeze-dried, the molded constructs were chemically crosslinked with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide/N-hydroxysuccinimide to maintain the mechanical integrity of the structure. The fabricated scaffolds were characterized in-depth by scanning electron microscopy, Fourier transform infrared spectroscopy, Brunauer-Emmett-Teller analysis, thermogravimetric analysis, and dynamic mechanical analysis in addition to the swelling, liquid uptake, and porosity tests. The extraction yield of mucilage was calculated to be 6.28% +/- 0.40% (n = 3). The swelling ratio of crosslinked quince seed mucilage-derived scaffolds was found to be 12,677.50% +/- 388.82% (n = 3), whereas the porosity of crosslinked quince seed mucilage-derived scaffolds was 83.43% +/- 2.84% (n = 3). The analyses confirmed the crosslinked quince seed mucilage-derived scaffolds to be possessed interconnected, highly porous structure. Afterward, human adipose-derived mesenchymal stem cells were seeded on the crosslinked quince seed mucilage-derived scaffolds, and the cell viability on the scaffolds was assessed with 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. The MTT results revealed the scaffolds not to be possessed any cytotoxic effect on seeded cells. Human adipose-derived mesenchymal stem cells adhesion and migration on the crosslinked quince seed mucilage-derived scaffolds were also evaluated histologically using hematoxylin and eosin staining in addition to scanning electron microscopy analysis. In conclusion, we believe that crosslinked quince seed mucilage-derived scaffolds have the potential to be an alternative to routinely used polysaccharides in regenerative medicine applications.
dc.description.sponsorshipCanakkale Onsekiz Mart University, Scientific Research Projects Coordination Unit [FYL-2018-2517]
dc.description.sponsorshipThe author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: The authors gratefully acknowledge financial support from Canakkale Onsekiz Mart University, Scientific Research Projects Coordination Unit (Project ID: FYL-2018-2517).
dc.identifier.doi10.1177/0883911520918390
dc.identifier.endpage253
dc.identifier.issn0883-9115
dc.identifier.issn1530-8030
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85084540457
dc.identifier.scopusqualityQ3
dc.identifier.startpage240
dc.identifier.urihttps://doi.org/10.1177/0883911520918390
dc.identifier.urihttps://hdl.handle.net/20.500.12428/26327
dc.identifier.volume35
dc.identifier.wosWOS:000532763800001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSage Publications Ltd
dc.relation.ispartofJournal of Bioactive and Compatible Polymers
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectQuince (Cydonia oblonga Miller) seeds
dc.subjectmucilage
dc.subjecthuman adipose-derived mesenchymal stem cells
dc.subjectbioengineered three-dimensional scaffolds
dc.subjecttissue engineering
dc.titleBioengineered three-dimensional physical constructs from quince seed mucilage for human adipose-derived mesenchymal stem cells
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
Yavuz Emre Arslan_Makale.pdf
Boyut:
758.68 KB
Biçim:
Adobe Portable Document Format
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Biyomühendislik Bölümü Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu