A Practical and Single-Use ITO-Pet Based Immunosensing Platform for Detection of Tumor Necrosis Factor-Alpha Biomarker

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dc.contributor.authorÖzcan, Burcu
dc.date.accessioned2025-01-27T19:15:21Z
dc.date.available2025-01-27T19:15:21Z
dc.date.issued2023
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThis investigation displays a novel, practical indium tin oxide- polyethylene terephthalate (ITO-PET) based electrochemical biosensor for the Tumor Necrosis Factor-alpha (TNF?) biomarker determination. The ITO-PET electrode is a very ad - vantageous preferred semiconductive electrode material. It has a lot of great features such as easy to prepare, cheapness, flexibility, stability. It also allows determining an analyte at very low concentrations and provides a great wide concentration range for analyte analysis. Electrochemical Impedance Spectroscopy (EIS) and Cyclic Voltammetry (CV) were used for the evaluation of biosensors, including the immobilization procedure, the investigation of the optimum conditions, and the characterization of biosensors. The immunosensor's electrode surface morphology during the immobilization process was observed using a Scanning Electron Microscope (SEM). In addition, Impedance measurement at a single frequency was used to characterize anti-TNF? and TNF? interactions (SFI). The clinical effectiveness of the developed biosensor was investiga - ted by testing it with real human serum samples. Moreover, the fabricated immunosensor presents long shelf life, analysis of the antigen concentrations at picogram level (0.02 pg mL-1 -2.56 pg mL-1), reproducibility, reusability and high sensitivity
dc.identifier.doi10.15671/hjbc.1215813
dc.identifier.endpage214
dc.identifier.issn1303-5002
dc.identifier.issn2687-475X
dc.identifier.issue2
dc.identifier.startpage201
dc.identifier.trdizinid1200440
dc.identifier.urihttps://doi.org/10.15671/hjbc.1215813
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1200440
dc.identifier.urihttps://hdl.handle.net/20.500.12428/14802
dc.identifier.volume51
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofHacettepe Journal of Biology and Chemistry
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_TRD_20250125
dc.subjectMikroskopi
dc.subjectBiyoloji
dc.subjectTıbbi İnformatik
dc.subjectGörüntüleme Bilimi ve Fotoğraf Teknolojisi
dc.subjectNörolojik Bilimler
dc.subjectOnkoloji
dc.titleA Practical and Single-Use ITO-Pet Based Immunosensing Platform for Detection of Tumor Necrosis Factor-Alpha Biomarker
dc.typeArticle

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