A Practical and Single-Use ITO-Pet Based Immunosensing Platform for Detection of Tumor Necrosis Factor-Alpha Biomarker
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Tarih
2023
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
This investigation displays a novel, practical indium tin oxide- polyethylene terephthalate (ITO-PET) based electrochemical biosensor for the Tumor Necrosis Factor-alpha (TNF?) biomarker determination. The ITO-PET electrode is a very ad - vantageous preferred semiconductive electrode material. It has a lot of great features such as easy to prepare, cheapness, flexibility, stability. It also allows determining an analyte at very low concentrations and provides a great wide concentration range for analyte analysis. Electrochemical Impedance Spectroscopy (EIS) and Cyclic Voltammetry (CV) were used for the evaluation of biosensors, including the immobilization procedure, the investigation of the optimum conditions, and the characterization of biosensors. The immunosensor's electrode surface morphology during the immobilization process was observed using a Scanning Electron Microscope (SEM). In addition, Impedance measurement at a single frequency was used to characterize anti-TNF? and TNF? interactions (SFI). The clinical effectiveness of the developed biosensor was investiga - ted by testing it with real human serum samples. Moreover, the fabricated immunosensor presents long shelf life, analysis of the antigen concentrations at picogram level (0.02 pg mL-1 -2.56 pg mL-1), reproducibility, reusability and high sensitivity
Açıklama
Anahtar Kelimeler
Mikroskopi, Biyoloji, Tıbbi İnformatik, Görüntüleme Bilimi ve Fotoğraf Teknolojisi, Nörolojik Bilimler, Onkoloji
Kaynak
Hacettepe Journal of Biology and Chemistry
WoS Q Değeri
Scopus Q Değeri
Cilt
51
Sayı
2
