Release of bifidogenic N-glycans from native bovine colostrum proteins by an endo-?-N-acetylglucosaminidase

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dc.authorid-en_US
dc.authorid-en_US
dc.authorid-en_US
dc.authorid0000-0003-4056-1673en_US
dc.authorscopusid57221201493en_US
dc.authorscopusid57210429018en_US
dc.authorscopusid57210425578en_US
dc.authorscopusid56688659000en_US
dc.authorwosid-en_US
dc.authorwosid-en_US
dc.authorwosid-en_US
dc.authorwosidT-8649-2018en_US
dc.contributor.authorBunyatratchata, Apichaya
dc.contributor.authorParc, Annabelle Le
dc.contributor.authorde Moura Bell, Juliana Maria Leite Nobrega
dc.contributor.authorCohen, Josh L.
dc.contributor.authorDuman, Hatice
dc.contributor.authorArslan, Ayşenur
dc.contributor.authorKaplan, Merve
dc.contributor.authorBarile, Daniela
dc.contributor.authorKarav, Sercan
dc.date.accessioned2023-10-24T05:50:19Z
dc.date.available2023-10-24T05:50:19Z
dc.date.issued2023en_US
dc.departmentFakülteler, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümü
dc.description.abstractMilk glycoproteins play various biological roles including antibacterial, antiviral activities, modulating immune responses in living organisms. Released N-glycans from milk glycoproteins act as growth substrates for infant-associated bifidobacteria, which are key members of the breastfed infant's gut. To date, the mechanisms, and contributions of glycans to the biological activities of glycoproteins remain to be elucidated. Only by testing both the released glycans and the deglycosylated protein in their native (i.e., non-denatured) form, can the individual contribution to the biological activity of glycoproteins be elucidated. However, for conventional enzymatic and chemical deglycosylation strategies to work efficiently, glycoprotein denaturation is required, which alters the protein native shape, hindering further investigations of its biological roles. An endo-β-N-acetylglucosaminidase (EndoBI-1) from Bifidobacterium longum subsp. infantis ATCC 15697 (B. infantis) was characterized as having the ability to release N-glycans from bovine milk glycoproteins efficiently, without the denaturation. In this study, the activity of EndoBI-1 was compared to a commercial enzyme to release N-glycans, the peptide-N-glycosidase F (PNGase F), using dairy glycoproteins as the substrate. The kinetic evaluation showed that EndoBI-1 displayed higher activity on native glycoproteins than PNGase F, with 0.036 mg/mL×min and 0.012 mg/mL×min glycan release, respectively. EndoBI-1 released a broader array of glycan structures compared to PNGase F from native glycoproteins. Thirty-two and fifteen distinct compositions were released from the native glycoproteins by EndoBI-1 and PNGase F, respectively, as characterized by advanced mass spectrometry. EndoBI-1 can be considered a promising enzyme for the release of N-glycans and their protein backbone in the native form, which will enable effective glycan release and will facilitate subsequent investigations to reveal their contribution to glycoproteins’ biological roles.en_US
dc.identifier.citationBunyatratchata, A., Parc, A. L., de Moura Bell, J. M. L. N., Cohen, J. L., Duman, H., Arslan, A., … Karav, S. (2023). Release of bifidogenic N-glycans from native bovine colostrum proteins by an endo-β-N-acetylglucosaminidase. Enzyme and Microbial Technology, 162, 110138. https://doi.org/10.1016/j.enzmictec.2022.110138en_US
dc.identifier.doi10.1016/j.enzmictec.2022.110138
dc.identifier.issn0141-0229
dc.identifier.issn1879-0909
dc.identifier.pmid36252443
dc.identifier.scopus2-s2.0-85140143535
dc.identifier.urihttps://doi.org/10.1016/j.enzmictec.2022.110138
dc.identifier.urihttps://hdl.handle.net/20.500.12428/4562
dc.identifier.volume162en_US
dc.identifier.wosWOS:000876958600005
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorDuman, Hatice
dc.institutionauthorArslan, Ayşenur
dc.institutionauthorKaplan, Merve
dc.institutionauthorKarav, Sercan
dc.language.isoen
dc.publisherElsevier Inc.en_US
dc.relation.ispartofEnzyme and Microbial Technologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.relation.tubitakinfo:eu-repo/grantAgreement/TUBITAK/SOBAG/117z132
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDeglycosylationen_US
dc.subjectEndoBI-1en_US
dc.subjectGlycoproteinsen_US
dc.subjectMass spectrometryen_US
dc.subjectN-linked glycansen_US
dc.subjectWhey proteinsen_US
dc.titleRelease of bifidogenic N-glycans from native bovine colostrum proteins by an endo-?-N-acetylglucosaminidase
dc.typeArticle

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