Apical sodium-glucose co-transport can be regulated by blood-borne glucose in the ruminal epithelium of sheep (Ovis aries, Merino breed)

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dc.authoridAtaşoğlu, Cengiz / 0000-0003-3207-1068
dc.contributor.authorAtaşoğlu, Cengiz
dc.contributor.authorGäbel, G.
dc.contributor.authorAschenbach, J. R.
dc.date.accessioned2025-01-27T21:05:25Z
dc.date.available2025-01-27T21:05:25Z
dc.date.issued2004
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThe intestinal Na-dependent d-glucose co-transporter (SGLT)-1 in sheep is under dietary regulation by luminal substrates. The aim of the present study was to find out whether the SGLT-1 in the forestomach of sheep is also regulated by sugars. Furthermore, the location of a possible glucosensor (luminal v. intracellular v. basolateral) was to be elucidated. Ruminal epithelia of sheep (Ovis aries, Merino breed) were pre-incubated in Ussing chambers with various substrates on the mucosal (i.e. luminal) or serosal (i.e. blood) side. This pre-incubation period was followed by a second pre-incubation period without the tested substrates (washout period). Thereafter, apical d-glucose uptake by ruminal epithelial cells was determined with 200 mumol d-[C-14]glucose/l in the absence or co-presence of the SGLT-1 inhibitor, phlorizin. Pre-incubation with d-glucose on the mucosal side had no significant effect on apical d-glucose uptake (P>0.05). In contrast, pre-incubation with d-glucose, d-mannose, 3-O-methyl-d-glucose or sucrose on the serosal side significantly increased d-glucose uptake compared with mannitol-treated controls (P<0.05). Serosal pre-incubation with cellobiose or d-xylose had no effect. The stimulation of d-glucose uptake by serosal d-glucose pre-incubation was concentration dependent, with maximal stimulation at about 10 mmol/l. We conclude that the ruminal SGLT-1 can be up-regulated in a concentration-dependent manner by blood-borne d-glucose via an extracellular sugar-sensing mechanism.
dc.identifier.doi10.1079/BJN20041265
dc.identifier.endpage783
dc.identifier.issn0007-1145
dc.identifier.issn1475-2662
dc.identifier.issue5
dc.identifier.pmid15533266
dc.identifier.scopus2-s2.0-9444280213
dc.identifier.scopusqualityQ1
dc.identifier.startpage777
dc.identifier.urihttps://doi.org/10.1079/BJN20041265
dc.identifier.urihttps://hdl.handle.net/20.500.12428/27636
dc.identifier.volume92
dc.identifier.wosWOS:000224963200005
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherCambridge Univ Press
dc.relation.ispartofBritish Journal of Nutrition
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectsodium-glucose-linked transport
dc.subjectglucosensor
dc.subjectrumen
dc.subjectsheep
dc.titleApical sodium-glucose co-transport can be regulated by blood-borne glucose in the ruminal epithelium of sheep (Ovis aries, Merino breed)
dc.typeArticle

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