Inhibitory effect of Curcumin on a cervical cancer cell line via the RAS/RAF signaling pathway

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dc.contributor.authorOzdemir, Ilhan
dc.contributor.authorZaman, Fuat
dc.contributor.authorBas, Dilek Dogan
dc.contributor.authorSari, Umut
dc.contributor.authorOzturk, Samil
dc.contributor.authorTuncer, Mehmet Cudi
dc.date.accessioned2025-05-29T02:57:38Z
dc.date.available2025-05-29T02:57:38Z
dc.date.issued2025
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractObjective. Cervical cancer has a very important place in female infertility and ranks fourth among cancers affecting women. Curcumin (CUR) is closely associated with the expression and activity of various regulatory proteins. It is also known that curcumin has preventive and therapeutic effects on various types of cancer. In this study, the anticancer activities of curcumin were demonstrated in the human cervical cancer cell line (HeLa). Methods. qRT-PCR and western blot analyses were used to evaluate mRNA and protein expression of curcumin in HeLa and immortalized human skin keratinocyte cell lines (HaCaT) (proliferation and apoptosis regulatory markers of the RAS/RAF signaling pathway). MTT analysis was performed, showing HeLa and HaCaT cell proliferation depending on the dose and duration of curcumin and doxorubicin. A wound scratch healing assay was applied to examine cell migration and invasion of HeLa after curcumin application. To determine the role of curcumin and doxorubicin in the apoptosis of HeLa cells, the mRNA levels of caspase-3 were examined by qRT-PCR. The results were analyzed with a one-way ANOVA SPSS 20.0 program. Results. CUR (IC50: 242.8 mu M) and DOX (IC50: 92.1 mu M) were determined to have the ability to inhibit the proliferation of HeLa cells and induce apoptosis over a 72-hour period and dose- dependently. Moreover, the results revealed that the mRNA and protein expression levels of RAF and RAS in HeLa cells were downregulated by CUR and DOX. Conclusions. The findings show that an alternative treatment method for cervical cancer can be developed with the application of CUR and DOX. Alternative methods for cervical cancer treatment may be developed using different methods in future studies.
dc.identifier.doi10.14670/HH-18-797
dc.identifier.endpage334
dc.identifier.issn0213-3911
dc.identifier.issn1699-5848
dc.identifier.issue3
dc.identifier.pmid39092501
dc.identifier.scopus2-s2.0-85219501465
dc.identifier.scopusqualityQ2
dc.identifier.startpage327
dc.identifier.urihttps://doi.org/10.14670/HH-18-797
dc.identifier.urihttps://hdl.handle.net/20.500.12428/30105
dc.identifier.volume40
dc.identifier.wosWOS:001445948600006
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherF Hernandez
dc.relation.ispartofHistology and Histopathology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250529
dc.subjectCurcumin
dc.subjectHeLa cells
dc.subjectCervical cancer
dc.subjectRAF/RAS signaling pathway
dc.titleInhibitory effect of Curcumin on a cervical cancer cell line via the RAS/RAF signaling pathway
dc.typeArticle

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