Dietary administration of Lactobacillus reuteri and Lactobacillus plantarum reduces whole body oxidative stress and increases immune response, digestive enzyme, growth performance and resistance of zebrafish (Danio rerio) against Aeromonas hydrophila infection

Basit Öğe Kaydı
dc.contributor.authorEsfahani, Delaram Eslimi
dc.contributor.authorAhmadifar, Mehdi
dc.contributor.authorEbrahimi, Pouya
dc.contributor.authorAhmadifar, Ehsan
dc.contributor.authorShohreh, Poulin
dc.contributor.authorAdineh, Hossein
dc.contributor.authorMoghadam, Mohsen Shahriari
dc.date.accessioned2025-01-27T20:38:49Z
dc.date.available2025-01-27T20:38:49Z
dc.date.issued2024
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThis study evaluated the individual and combined effects of L. plantarum and L. reuteri on the growth performance, digestive enzymes, antioxidant, and immunity-related genes in zebrafish. Six hundred zebrafish (38.19 +/- 1.4 mg) were randomized into twelve tanks (50 fish per), and fed prepared diets comprising un-supplemented (T0), 1 x10(8) CFU/g L. plantarum (T1), 1 x10(11) CFU/kg L. reuteri (T2), and 1 x10(8) CFU/g L. plantarum + 1 x10(11) CFU/kg L. reuteri (T3) for 8 weeks. Experimental diets had meaningful effects on weight gain (WG), final weight (FW), and the highest WG and FW were seen in T3. Fish-fed supplemented diets had higher amylase and lipase activities. No significant differences were seen in protease, pepsin, chymotrypsin, and trypsin activities between treatments. The supplemented groups showed a significant increase in lysozyme activity and total immunoglobulin levels. Meaningful differences were noticed in ACH50, lysozyme activity, total immunoglobulin, and IgM levels. ALT levels were markedly higher in T2 and T3. Catalase (CAT), total antioxidant, and glutathione peroxidase (GPX) activity were significantly impacted by the experimental diets. The experimental diets showed no marked impact on superoxide dismutase (SOD) activity, and malondialdehyde (MDA) values. Relative expressions of CAT, GPX, and SOD genes were notably upper in T3. Moreover, IL1, LYZ, and TNF alpha gene expressions were significantly enhanced in T3 diets. The challenge test with A. hydrophila showed that zebrafish-fed L. plantarum and L. reuteri had a lower mortality rate than the control. Consequently, the combination of L. reuteri and L. plantarum is proposed to improve growth efficiency, immunity, and reduce the negative effects of A. hydrophila infection in zebrafish.
dc.description.sponsorshipKharazmi University (Iran)
dc.description.sponsorshipThis research was supported by the Kharazmi University (Iran)
dc.identifier.doi10.2478/aoas-2024-0066
dc.identifier.issn1642-3402
dc.identifier.issn2300-8733
dc.identifier.scopus2-s2.0-85199567598
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.2478/aoas-2024-0066
dc.identifier.urihttps://hdl.handle.net/20.500.12428/23762
dc.identifier.wosWOS:001272924600001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWalter De Gruyter Gmbh
dc.relation.ispartofAnnals of Animal Science
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectLactobacillus reuteri
dc.subjectLactobacillus plantarum
dc.subjectaquaculture
dc.subjectDanio rerio
dc.subjectprobiotics
dc.titleDietary administration of Lactobacillus reuteri and Lactobacillus plantarum reduces whole body oxidative stress and increases immune response, digestive enzyme, growth performance and resistance of zebrafish (Danio rerio) against Aeromonas hydrophila infection
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu