A high sensitive and cost-effective disposable biosensor for adiponectin determination in real human serum samples
Yükleniyor...
Dosyalar
Tarih
2021
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
One of the adipose tissue cytokines, adiponectin is a newly defined adipocytokin, which is involved in insulin,
glucose and adipocyte metabolisms. It has been determined that the amount of adiponectin in the circulatory
system decreases in obese and type 2 diabetic patients. In addition to this decrease, many diseases such as heart
diseases, high blood pressure, diabetes, joint diseases, high cholesterol, respiratory disease, gallbladder diseases
are associated with obesity. Early detection of a decrease in the level of Adiponectin with a biosensor may be a
new target for the prevention and treatment of cardiovascular diseases, immune disorder, joint disorders,
especially obesity-related pathologies. In the scope of this study, poly ethylene terephthalate (PET) sheets
covered with indium tin oxide (ITO), a unique transparent conductive material, was used as a working electrode
which, introduced an extremely cheap and flexible material for this purpose. 3-Glycidoxypropyltrimethoxysilane
(3-GOPS), an effective organosilane agent, produced a stable layer to immobilize anti-Adiponectin onto the
working electrode surface. The immobilization of anti-Adiponectin onto ITO sheets were proved by the tech-
niques, Fourier-transform infrared spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), and Atomic Force
Microscopy (AFM). The immunosensor prepared under optimal conditions gave a response in a wide range of
concentrations (25 pg/mL–2500 pg/mL with a LOD value of 148 pg/mL) of Adiponectin. Finally, the designed
biosensor was applied to real human serum samples and successful results were obtained.
Açıklama
Anahtar Kelimeler
Adiponectin, Adipocytokin, Obesity, Biosensor, Indium tin oxide
Kaynak
Sensors and Actuators: B. Chemical
WoS Q Değeri
Q1
Scopus Q Değeri
Cilt
328
Sayı
Künye
İnce, B., & Sezgintürk, M. K. (2021). A high sensitive and cost-effective disposable biosensor for adiponectin determination in real human serum samples. Sensors and Actuators, B: Chemical, 328 doi:10.1016/j.snb.2020.129051
