Molecular characterization of tocopherol biosynthesis genes from Olea europaea (L.) cv. Ayvalik

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dc.authoridTaşkın, Kemal Melih / 0000-0002-3746-0508
dc.authoridSezer, Fatih / 0000-0002-9436-0191
dc.contributor.authorSezer, Fatih
dc.contributor.authorTaşkın, Kemal Melih
dc.date.accessioned2025-01-27T21:04:22Z
dc.date.available2025-01-27T21:04:22Z
dc.date.issued2017
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractVitamin E is a group of compounds that includes metabolites known as tocopherols, which have high antioxidant activities. Tocopherols are synthesized in plants, and their beneficial effects on human health have been reported for diseases such as coronary heart disease and cancer. Here we report the full-length transcripts encoding vitamin E biosynthetic enzymes from fruit mesocarp tissues using the rapid amplification of cDNA ends (RACE) method for the first time in olives. We characterized the structure of the genes 4-hydroxyphenylpyruvate dioxygenase (PDS1), homogentisate phytyltransferase 1 (HPT1), vitamin E defective 3 (VTE3), tocopherol cyclase (VTE1), and gamma tocopherol methyltransferase (GTMT), which are responsible for tocopherol biosynthesis in the olive cultivar Ayvalik. Although PDS1 is widespread in all organisms, HPT1, VTE3, VTE1, and GTMT are only present in photosynthetic organisms. We isolated total RNA from the dissected mesocarp tissues of fruit collected at 15-day intervals between October and December 2014. We compared the expression levels of genes using quantitative RT-PCR and determined the tocopherol content using the high-performance liquid chromatography-fluorescence detection (HPLC-FLD) technique during fruit maturation in the Ayvalik cultivar. High OeHPT1, OeVTE3, OeVTE1, and OeGTMT expression was noted in young fruit. However, the OePDS1 mRNA did not exhibit significant expression changes during maturation. The alpha-tocopherol content varied between 26.78 and 21.05 mg/100 g and was highest at the early stages of fruit development. In addition, expression studies and tocopherol content revealed that tocopherol biosynthesis in olive is more active at the early stages of fruit maturation.
dc.description.sponsorshipTUBITAK (the Scientific and Technological Research Council of Turkey) [113O724]
dc.description.sponsorshipThe authors thank TUBITAK (the Scientific and Technological Research Council of Turkey) for its financial support for this project (113O724). We also thank the Edremit Olive Production, Training, and Gene Center for providing the plant materials.
dc.identifier.doi10.3906/bot-1701-41
dc.identifier.endpage+
dc.identifier.issn1300-008X
dc.identifier.issn1303-6106
dc.identifier.issue6
dc.identifier.scopus2-s2.0-85035150129
dc.identifier.scopusqualityQ2
dc.identifier.startpage559
dc.identifier.trdizinid532275
dc.identifier.urihttps://doi.org/10.3906/bot-1701-41
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/532275
dc.identifier.urihttps://hdl.handle.net/20.500.12428/27623
dc.identifier.volume41
dc.identifier.wosWOS:000418290200003
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherTubitak Scientific & Technological Research Council Turkey
dc.relation.ispartofTurkish Journal of Botany
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectOlea europaea
dc.subjecttocopherol
dc.subjectvitamin E biosynthesis
dc.subjectgene expression
dc.titleMolecular characterization of tocopherol biosynthesis genes from Olea europaea (L.) cv. Ayvalik
dc.typeArticle

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